Abstract

The structural and molecular heterogeneities of biological tissues demand the interrogation of the samples with multiple energy sources and provide visualization capabilities at varying spatial resolution and depth scales for obtaining complementary diagnostic information. A novel multi-modal imaging approach that uses optical and acoustic energies to perform photoacoustic, ultrasound and fluorescence imaging at multiple resolution scales from the tissue surface and depth is proposed in this paper. The system comprises of two distinct forms of hardware level integration so as to have an integrated imaging system under a single instrumentation set-up. The experimental studies show that the system is capable of mapping high resolution fluorescence signatures from the surface, optical absorption and acoustic heterogeneities along the depth (>2cm) of the tissue at multi-scale resolution (<1µm to <0.5mm).

© 2014 Optical Society of America

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. R. Weissleder and M. J. Pittet, “Imaging in the era of molecular oncology,” Nature452(7187), 580–589 (2008).
    [CrossRef] [PubMed]
  2. V. V. Tuchin, “Tissue optics: Light scattering methods and instruments for medical diagnosis ed 2,” Bellingham SPIE Publications (2007).
  3. S. R. Cherry, “Multimodality in vivo imaging systems: Twice the power or double the trouble?” Annu. Rev. Biomed. Eng.8(1), 35–62 (2006).
    [CrossRef] [PubMed]
  4. S. R. Cherry, “Multimodality imaging: beyond PET/CT and SPECT/CT,” Semin. Nucl. Med.39(5), 348–353 (2009).
    [CrossRef] [PubMed]
  5. D. W. Townsend, “Multimodality imaging of structure and function,” Phys. Med. Biol.53(4), R1–R39 (2008).
    [CrossRef] [PubMed]
  6. R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
    [CrossRef] [PubMed]
  7. J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
    [CrossRef] [PubMed]
  8. G. Rousseau, A. Blouin, and J.-P. Monchalin, “Non-contact photoacoustic tomography and ultrasonography for tissue imaging,” Biomed. Opt. Express3(1), 16–25 (2012).
    [CrossRef] [PubMed]
  9. L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
    [CrossRef] [PubMed]
  10. B.-Y. Hsieha and P.-C. Lia, “Real-time intravascular ultrasound/photoacoustic imaging system with omni-directional light excitation,” Photons Plus Ultrasound: Imaging and Sensing8223, 822319 (2012).
  11. A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
    [CrossRef] [PubMed]
  12. U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).
  13. T. Harrison, J. C. Ranasinghesagara, H. Lu, K. Mathewson, A. Walsh, and R. J. Zemp, “Combined photoacoustic and ultrasound biomicroscopy,” Opt. Express17(24), 22041–22046 (2009).
    [CrossRef] [PubMed]
  14. W. Wei, X. Li, Q. Zhou, K. K. Shung, and Z. Chen, “Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging,” J. Biomed. Opt.16(10), 106001 (2011).
    [CrossRef] [PubMed]
  15. L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
    [CrossRef] [PubMed]
  16. X. Li, L. Xi, R. Jiang, L. Yao, and H. Jiang, “Integrated diffuse optical tomography and photoacoustic tomography: phantom validations,” Biomed. Opt. Express2(8), 2348–2353 (2011).
    [CrossRef] [PubMed]
  17. D. Razansky and V. Ntziachristos, “Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion,” Med. Phys.34(11), 4293–4301 (2007).
    [CrossRef] [PubMed]
  18. Y. Wang, S. Hu, K. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, “In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure,” Opt. Lett.36(7), 1029–1031 (2011).
    [CrossRef] [PubMed]
  19. Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
    [CrossRef] [PubMed]
  20. S. Jiao, Z. Xie, H. F. Zhang, and C. A. Puliafito, “Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography,” Opt. Lett.34(19), 2961–2963 (2009).
    [CrossRef] [PubMed]
  21. E. Z. Zhang, “Multimodal simultaneous photoacoustic tomography, optical resolution microscopy, and OCT system,” Photons Plus Ultrasound: Imaging and Sensing7564, 75640–75647 (2010).
  22. T. Liu, Q. Wei, J. Wang, S. Jiao, and H. F. Zhang, “Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen,” Biomed. Opt. Express2(5), 1359–1365 (2011).
    [CrossRef] [PubMed]
  23. C. Lee, S. Han, S. Kim, M. Jeon, M. Y. Jeon, C. Kim, and J. Kim, “Combined photoacoustic and optical coherence tomography using a single near-infrared supercontinuum laser source,” Appl. Opt.52(9), 1824–1828 (2013).
    [CrossRef] [PubMed]
  24. X. Zhang, H. F. Zhang, and S. Jiao, “Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source,” J. Biomed. Opt.17(3), 0305021 (2011).
  25. Y. Yang, X. Li, T. Wang, P. D. Kumavor, A. Aguirre, K. K. Shung, Q. Zhou, M. Sanders, M. Brewer, and Q. Zhu, “Integrated optical coherence tomography, ultrasound and photoacoustic imaging for ovarian tissue characterization,” Biomed. Opt. Express2(9), 2551–2561 (2011).
    [CrossRef] [PubMed]
  26. H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
    [CrossRef] [PubMed]
  27. D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).
  28. S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).
  29. S. Emelianov, S. Aglyamov, A. Karpiouk, S. Mallidi, S. Park, S. Sethuraman, J. Shah, R. Smalling, J. Rubin, and W. Scott, “1E-5 Synergy and Applications of Combined Ultrasound, Elasticity, and Photoacoustic Imaging,” in Ultrason, pp 405-415 (2006).
  30. I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
    [CrossRef] [PubMed]
  31. P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
    [CrossRef] [PubMed]
  32. I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
    [CrossRef]
  33. J. Joseph, V. M. Murukeshan, K. Sathiyamoorthy, and S. W. Lye, “Integrated laser photoacoustic, ultrasound and fluorescence imaging (PAUSFI) for diagnostic bio-imaging applications,” Laser Phys. (to be published).
  34. R. Choe, “Diffuse optical tomography and spectroscopy of breast cancer and fetal brain,” http://www.physics.upenn.edu/yodhlab/theses/regine_choe.pdf .
    [CrossRef]
  35. American national standard for safe use of lasers (ANSI Z136.1–2007), (Laser Institute of America, 2000).

2013 (5)

L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
[CrossRef] [PubMed]

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

C. Lee, S. Han, S. Kim, M. Jeon, M. Y. Jeon, C. Kim, and J. Kim, “Combined photoacoustic and optical coherence tomography using a single near-infrared supercontinuum laser source,” Appl. Opt.52(9), 1824–1828 (2013).
[CrossRef] [PubMed]

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

2012 (6)

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
[CrossRef] [PubMed]

G. Rousseau, A. Blouin, and J.-P. Monchalin, “Non-contact photoacoustic tomography and ultrasonography for tissue imaging,” Biomed. Opt. Express3(1), 16–25 (2012).
[CrossRef] [PubMed]

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

B.-Y. Hsieha and P.-C. Lia, “Real-time intravascular ultrasound/photoacoustic imaging system with omni-directional light excitation,” Photons Plus Ultrasound: Imaging and Sensing8223, 822319 (2012).

2011 (6)

2010 (3)

E. Z. Zhang, “Multimodal simultaneous photoacoustic tomography, optical resolution microscopy, and OCT system,” Photons Plus Ultrasound: Imaging and Sensing7564, 75640–75647 (2010).

D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

2009 (3)

2008 (3)

D. W. Townsend, “Multimodality imaging of structure and function,” Phys. Med. Biol.53(4), R1–R39 (2008).
[CrossRef] [PubMed]

R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
[CrossRef] [PubMed]

R. Weissleder and M. J. Pittet, “Imaging in the era of molecular oncology,” Nature452(7187), 580–589 (2008).
[CrossRef] [PubMed]

2007 (1)

D. Razansky and V. Ntziachristos, “Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion,” Med. Phys.34(11), 4293–4301 (2007).
[CrossRef] [PubMed]

2006 (1)

S. R. Cherry, “Multimodality in vivo imaging systems: Twice the power or double the trouble?” Annu. Rev. Biomed. Eng.8(1), 35–62 (2006).
[CrossRef] [PubMed]

2005 (1)

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

2004 (1)

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Aglyamov, S. R.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Aguirre, A.

Alqasemi, U.

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

Bates, D.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Bauer, D. R.

L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
[CrossRef] [PubMed]

Blouin, A.

Bossy, E.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Brands, P. J.

R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
[CrossRef] [PubMed]

Brewer, M.

Chen, Z.

W. Wei, X. Li, Q. Zhou, K. K. Shung, and Z. Chen, “Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging,” J. Biomed. Opt.16(10), 106001 (2011).
[CrossRef] [PubMed]

Cherry, S. R.

S. R. Cherry, “Multimodality imaging: beyond PET/CT and SPECT/CT,” Semin. Nucl. Med.39(5), 348–353 (2009).
[CrossRef] [PubMed]

S. R. Cherry, “Multimodality in vivo imaging systems: Twice the power or double the trouble?” Annu. Rev. Biomed. Eng.8(1), 35–62 (2006).
[CrossRef] [PubMed]

DelRio, S. P.

D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).

Emelianov, S.

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

Emelianov, S. Y.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Erpelding, T. N.

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

Farahi, S.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Foster, F. S.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Frenz, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

Graf, I. M.

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

Grobmyer, S.

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

Hajireza, P.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
[CrossRef] [PubMed]

Han, S.

Harrison, T.

Heinmiller, A.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Hirson, D.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Hsieha, B.-Y.

B.-Y. Hsieha and P.-C. Lia, “Real-time intravascular ultrasound/photoacoustic imaging system with omni-directional light excitation,” Photons Plus Ultrasound: Imaging and Sensing8223, 822319 (2012).

Hu, S.

Y. Wang, S. Hu, K. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, “In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure,” Opt. Lett.36(7), 1029–1031 (2011).
[CrossRef] [PubMed]

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

Huignard, J. P.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Jaeger, M.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

Jankovic, L.

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

Jeon, M.

Jeon, M. Y.

Jiang, H.

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

X. Li, L. Xi, R. Jiang, L. Yao, and H. Jiang, “Integrated diffuse optical tomography and photoacoustic tomography: phantom validations,” Biomed. Opt. Express2(8), 2348–2353 (2011).
[CrossRef] [PubMed]

Jiang, R.

Jiao, S.

Joseph, J.

J. Joseph, V. M. Murukeshan, K. Sathiyamoorthy, and S. W. Lye, “Integrated laser photoacoustic, ultrasound and fluorescence imaging (PAUSFI) for diagnostic bio-imaging applications,” Laser Phys. (to be published).

Karpiouk, A.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Ke, H.

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

Kim, C.

C. Lee, S. Han, S. Kim, M. Jeon, M. Y. Jeon, C. Kim, and J. Kim, “Combined photoacoustic and optical coherence tomography using a single near-infrared supercontinuum laser source,” Appl. Opt.52(9), 1824–1828 (2013).
[CrossRef] [PubMed]

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

Kim, J.

Kim, S.

C. Lee, S. Han, S. Kim, M. Jeon, M. Y. Jeon, C. Kim, and J. Kim, “Combined photoacoustic and optical coherence tomography using a single near-infrared supercontinuum laser source,” Appl. Opt.52(9), 1824–1828 (2013).
[CrossRef] [PubMed]

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

Kolkman, R. G. M.

R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
[CrossRef] [PubMed]

Kruger, R. A.

D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).

Kumavor, P.

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

Kumavor, P. D.

Lam, R. B.

D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).

Lee, C.

Lemor, R.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

Li, H.

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

Li, X.

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

X. Li, L. Xi, R. Jiang, L. Yao, and H. Jiang, “Integrated diffuse optical tomography and photoacoustic tomography: phantom validations,” Biomed. Opt. Express2(8), 2348–2353 (2011).
[CrossRef] [PubMed]

W. Wei, X. Li, Q. Zhou, K. K. Shung, and Z. Chen, “Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging,” J. Biomed. Opt.16(10), 106001 (2011).
[CrossRef] [PubMed]

Y. Yang, X. Li, T. Wang, P. D. Kumavor, A. Aguirre, K. K. Shung, Q. Zhou, M. Sanders, M. Brewer, and Q. Zhu, “Integrated optical coherence tomography, ultrasound and photoacoustic imaging for ovarian tissue characterization,” Biomed. Opt. Express2(9), 2551–2561 (2011).
[CrossRef] [PubMed]

Lia, P.-C.

B.-Y. Hsieha and P.-C. Lia, “Real-time intravascular ultrasound/photoacoustic imaging system with omni-directional light excitation,” Photons Plus Ultrasound: Imaging and Sensing8223, 822319 (2012).

Liu, C.

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

Liu, T.

Lu, H.

Lye, S. W.

J. Joseph, V. M. Murukeshan, K. Sathiyamoorthy, and S. W. Lye, “Integrated laser photoacoustic, ultrasound and fluorescence imaging (PAUSFI) for diagnostic bio-imaging applications,” Laser Phys. (to be published).

Maslov, K.

Y. Wang, S. Hu, K. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, “In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure,” Opt. Lett.36(7), 1029–1031 (2011).
[CrossRef] [PubMed]

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

Mathewson, K.

Monchalin, J.-P.

Montilla, L. G.

L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
[CrossRef] [PubMed]

Moser, C.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Motamedi, M.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Murukeshan, V. M.

J. Joseph, V. M. Murukeshan, K. Sathiyamoorthy, and S. W. Lye, “Integrated laser photoacoustic, ultrasound and fluorescence imaging (PAUSFI) for diagnostic bio-imaging applications,” Laser Phys. (to be published).

Needles, A.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Niederhauser, J. J.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

Ntziachristos, V.

D. Razansky and V. Ntziachristos, “Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion,” Med. Phys.34(11), 4293–4301 (2007).
[CrossRef] [PubMed]

Olafsson, R.

L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
[CrossRef] [PubMed]

Oraevsky, A. A.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Papadopoulos, I. N.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Pittet, M. J.

R. Weissleder and M. J. Pittet, “Imaging in the era of molecular oncology,” Nature452(7187), 580–589 (2008).
[CrossRef] [PubMed]

Psaltis, D.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Puliafito, C. A.

Ranasinghesagara, J. C.

Razansky, D.

D. Razansky and V. Ntziachristos, “Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion,” Med. Phys.34(11), 4293–4301 (2007).
[CrossRef] [PubMed]

Reinecke, D. R.

D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).

Rousseau, G.

Sanders, M.

Sathiyamoorthy, K.

J. Joseph, V. M. Murukeshan, K. Sathiyamoorthy, and S. W. Lye, “Integrated laser photoacoustic, ultrasound and fluorescence imaging (PAUSFI) for diagnostic bio-imaging applications,” Laser Phys. (to be published).

Schmitt, R.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Scott, W.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Sethuraman, S.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Shah, J.

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Shao, P.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
[CrossRef] [PubMed]

Shi, W.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
[CrossRef] [PubMed]

Shung, K. K.

Simandoux, O.

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Smalling, R.

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

Steenbergen, W.

R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
[CrossRef] [PubMed]

Sun, J.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Theodoropoulos, C.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Townsend, D. W.

D. W. Townsend, “Multimodality imaging of structure and function,” Phys. Med. Biol.53(4), R1–R39 (2008).
[CrossRef] [PubMed]

van Leeuwen, T. G.

R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
[CrossRef] [PubMed]

Walsh, A.

Wang, B.

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

Wang, J.

Wang, L. V.

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

Y. Wang, S. Hu, K. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, “In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure,” Opt. Lett.36(7), 1029–1031 (2011).
[CrossRef] [PubMed]

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

Wang, T.

Wang, Y.

Y. Wang, S. Hu, K. Maslov, Y. Zhang, Y. Xia, and L. V. Wang, “In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure,” Opt. Lett.36(7), 1029–1031 (2011).
[CrossRef] [PubMed]

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

Weber, P.

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

Wei, Q.

Wei, W.

W. Wei, X. Li, Q. Zhou, K. K. Shung, and Z. Chen, “Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging,” J. Biomed. Opt.16(10), 106001 (2011).
[CrossRef] [PubMed]

Weissleder, R.

R. Weissleder and M. J. Pittet, “Imaging in the era of molecular oncology,” Nature452(7187), 580–589 (2008).
[CrossRef] [PubMed]

Witte, R. S.

L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
[CrossRef] [PubMed]

Xi, L.

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

X. Li, L. Xi, R. Jiang, L. Yao, and H. Jiang, “Integrated diffuse optical tomography and photoacoustic tomography: phantom validations,” Biomed. Opt. Express2(8), 2348–2353 (2011).
[CrossRef] [PubMed]

Xia, Y.

Xie, Z.

Yang, Y.

Yao, L.

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

X. Li, L. Xi, R. Jiang, L. Yao, and H. Jiang, “Integrated diffuse optical tomography and photoacoustic tomography: phantom validations,” Biomed. Opt. Express2(8), 2348–2353 (2011).
[CrossRef] [PubMed]

Yin, M.

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

Yuan, G.

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

Zanganeh, S.

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

Zemp, R. J.

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
[CrossRef] [PubMed]

T. Harrison, J. C. Ranasinghesagara, H. Lu, K. Mathewson, A. Walsh, and R. J. Zemp, “Combined photoacoustic and ultrasound biomicroscopy,” Opt. Express17(24), 22041–22046 (2009).
[CrossRef] [PubMed]

Zhang, E. Z.

E. Z. Zhang, “Multimodal simultaneous photoacoustic tomography, optical resolution microscopy, and OCT system,” Photons Plus Ultrasound: Imaging and Sensing7564, 75640–75647 (2010).

Zhang, H. F.

Zhang, X.

X. Zhang, H. F. Zhang, and S. Jiao, “Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source,” J. Biomed. Opt.17(3), 0305021 (2011).

Zhang, Y.

Zhou, Q.

Zhu, Q.

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

Y. Yang, X. Li, T. Wang, P. D. Kumavor, A. Aguirre, K. K. Shung, Q. Zhou, M. Sanders, M. Brewer, and Q. Zhu, “Integrated optical coherence tomography, ultrasound and photoacoustic imaging for ovarian tissue characterization,” Biomed. Opt. Express2(9), 2551–2561 (2011).
[CrossRef] [PubMed]

Annu. Rev. Biomed. Eng. (1)

S. R. Cherry, “Multimodality in vivo imaging systems: Twice the power or double the trouble?” Annu. Rev. Biomed. Eng.8(1), 35–62 (2006).
[CrossRef] [PubMed]

Appl. Opt. (1)

Appl. Phys. Lett. (1)

I. N. Papadopoulos, O. Simandoux, S. Farahi, J. P. Huignard, E. Bossy, D. Psaltis, and C. Moser, “Optical-resolution photoacoustic microscopy by use of a multimode fiber,” Appl. Phys. Lett.102(21), 211106 (2013).
[CrossRef]

Biomed. Opt. Express (4)

IEEE Trans. Biomed. Eng. (1)

Y. Wang, K. Maslov, C. Kim, S. Hu, and L. V. Wang, “Integrated photoacoustic and fluorescence confocal microscopy,” IEEE Trans. Biomed. Eng.57(10), 2576–2578 (2010).
[CrossRef] [PubMed]

IEEE Trans. Med. Imaging (1)

J. J. Niederhauser, M. Jaeger, R. Lemor, P. Weber, and M. Frenz, “Combined ultrasound and optoacoustic system for real-time high-contrast vascular imaging in vivo,” IEEE Trans. Med. Imaging24(4), 436–440 (2005).
[CrossRef] [PubMed]

IEEE Trans. Ultrason. Ferroelectr. Freq. Control (1)

A. Needles, A. Heinmiller, J. Sun, C. Theodoropoulos, D. Bates, D. Hirson, M. Yin, and F. S. Foster, “Development and initial application of a fully integrated photoacoustic micro-ultrasound system,” IEEE Trans. Ultrason. Ferroelectr. Freq. Control60(5), 888–897 (2013).
[CrossRef] [PubMed]

J. Biomed. Opt. (5)

W. Wei, X. Li, Q. Zhou, K. K. Shung, and Z. Chen, “Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging,” J. Biomed. Opt.16(10), 106001 (2011).
[CrossRef] [PubMed]

R. G. M. Kolkman, P. J. Brands, W. Steenbergen, and T. G. van Leeuwen, “Real-time in vivo photoacoustic and ultrasound imaging,” J. Biomed. Opt.13(5), 050510 (2008).
[CrossRef] [PubMed]

H. Ke, T. N. Erpelding, L. Jankovic, C. Liu, and L. V. Wang, “Performance characterization of an integrated ultrasound, photoacoustic, and thermoacoustic imaging system,” J. Biomed. Opt.17(5), 056010 (2012).
[CrossRef] [PubMed]

X. Zhang, H. F. Zhang, and S. Jiao, “Optical coherence photoacoustic microscopy: accomplishing optical coherence tomography and photoacoustic microscopy with a single light source,” J. Biomed. Opt.17(3), 0305021 (2011).

P. Shao, W. Shi, P. Hajireza, and R. J. Zemp, “Integrated micro-endoscopy system for simultaneous fluorescence and optical-resolution photoacoustic imaging,” J. Biomed. Opt.17(7), 076024 (2012).
[CrossRef] [PubMed]

Med. Phys. (2)

L. Xi, X. Li, L. Yao, S. Grobmyer, and H. Jiang, “Design and evaluation of a hybrid photoacoustic tomography and diffuse optical tomography system for breast cancer detection,” Med. Phys.39(5), 2584–2594 (2012).
[CrossRef] [PubMed]

D. Razansky and V. Ntziachristos, “Hybrid photoacoustic fluorescence molecular tomography using finite-element-based inversion,” Med. Phys.34(11), 4293–4301 (2007).
[CrossRef] [PubMed]

Nature (1)

R. Weissleder and M. J. Pittet, “Imaging in the era of molecular oncology,” Nature452(7187), 580–589 (2008).
[CrossRef] [PubMed]

Opt. Express (1)

Opt. Lett. (2)

Photons Plus Ultrasound: Imaging and Sensing (5)

U. Alqasemi, H. Li, G. Yuan, P. Kumavor, S. Zanganeh, and Q. Zhu, “Real-time interlaced ultrasound and photoacoustic system for in vivo ovarian tissue imaging,” Photons Plus Ultrasound: Imaging and Sensing8581, 85814S (2013).

B.-Y. Hsieha and P.-C. Lia, “Real-time intravascular ultrasound/photoacoustic imaging system with omni-directional light excitation,” Photons Plus Ultrasound: Imaging and Sensing8223, 822319 (2012).

E. Z. Zhang, “Multimodal simultaneous photoacoustic tomography, optical resolution microscopy, and OCT system,” Photons Plus Ultrasound: Imaging and Sensing7564, 75640–75647 (2010).

D. R. Reinecke, R. A. Kruger, R. B. Lam, and S. P. DelRio, “Co-registered photoacoustic, thermoacoustic, and ultrasound mouse imaging,” Photons Plus Ultrasound: Imaging and Sensing7564, 756420 (2010).

S. Y. Emelianov, S. R. Aglyamov, J. Shah, S. Sethuraman, W. Scott, R. Schmitt, M. Motamedi, A. Karpiouk, and A. A. Oraevsky, “Combined ultrasound, optoacoustic, and elasticity imaging,” Photons Plus Ultrasound: Imaging and Sensing5320, 101–112 (2004).

Phys. Med. Biol. (2)

D. W. Townsend, “Multimodality imaging of structure and function,” Phys. Med. Biol.53(4), R1–R39 (2008).
[CrossRef] [PubMed]

L. G. Montilla, R. Olafsson, D. R. Bauer, and R. S. Witte, “Real-time photoacoustic and ultrasound imaging: a simple solution for clinical ultrasound systems with linear arrays,” Phys. Med. Biol.58(1), N1–N12 (2013).
[CrossRef] [PubMed]

Semin. Nucl. Med. (1)

S. R. Cherry, “Multimodality imaging: beyond PET/CT and SPECT/CT,” Semin. Nucl. Med.39(5), 348–353 (2009).
[CrossRef] [PubMed]

Ultrasonics (1)

I. M. Graf, S. Kim, B. Wang, R. Smalling, and S. Emelianov, “Noninvasive detection of intimal xanthoma using combined ultrasound, strain rate and photoacoustic imaging,” Ultrasonics52(3), 435–441 (2012).
[CrossRef] [PubMed]

Other (5)

J. Joseph, V. M. Murukeshan, K. Sathiyamoorthy, and S. W. Lye, “Integrated laser photoacoustic, ultrasound and fluorescence imaging (PAUSFI) for diagnostic bio-imaging applications,” Laser Phys. (to be published).

R. Choe, “Diffuse optical tomography and spectroscopy of breast cancer and fetal brain,” http://www.physics.upenn.edu/yodhlab/theses/regine_choe.pdf .
[CrossRef]

American national standard for safe use of lasers (ANSI Z136.1–2007), (Laser Institute of America, 2000).

S. Emelianov, S. Aglyamov, A. Karpiouk, S. Mallidi, S. Park, S. Sethuraman, J. Shah, R. Smalling, J. Rubin, and W. Scott, “1E-5 Synergy and Applications of Combined Ultrasound, Elasticity, and Photoacoustic Imaging,” in Ultrason, pp 405-415 (2006).

V. V. Tuchin, “Tissue optics: Light scattering methods and instruments for medical diagnosis ed 2,” Bellingham SPIE Publications (2007).

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (5)

Fig. 1
Fig. 1

Block diagram of the integrated ultrasound and photoacoustic imaging system high voltage multiplexer (HV MUX), transmit and receive (T/R), low noise amplifier (LNA), voltage controlled attenuator (VCAT), programmable gain amplifier (PGA) and low pass filter (LPF), analog to digital converted (ADC), delay and sum (DAS), receive (RX), beamformer (BF), transmit (TX), radio frequency (RF), universal serial bus (USB), graphics processing unit (GPU).

Fig. 2
Fig. 2

Silicone phantom; a) Schematic of the phantom tissue with simulated target locations, b) Absorption spectra of the absorbing dyes, c) Fluorescence spectrum of the fluorescent microbead, d) Emission spectrum of the microbead at 519 nm excitation.

Fig. 3
Fig. 3

Schematic of the integrated photoacoustic, ultrasound and fluorescence imaging system.

Fig. 4
Fig. 4

Resolution of the PA/US imaging system; a) Axial resolution, b) In–plane resolution

Fig. 5
Fig. 5

Images obtained from the integrated photoacoustic, ultrasound and fluorescence Imaging system: a) Fluorescence image, b) Photoacoustic image, c) Ultrasound image

Metrics