Abstract

We accomplished spectral domain optical coherence tomography and auto-fluorescence microscopy for imaging the retina with a single broadband light source centered at 480 nm. This technique is able to provide simultaneous structural imaging and lipofuscin molecular contrast of the retina. Since the two imaging modalities are provided by the same group of photons, their images are intrinsically registered. To test the capabilities of the technique we periodically imaged the retinas of the same rats for four weeks. The images successfully demonstrated lipofuscin accumulation in the retinal pigment epithelium with aging. The experimental results showed that the dual-modal imaging system can be a potentially powerful tool in the study of age-related degenerative retinal diseases.

© 2014 Optical Society of America

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2014 (1)

H. Pahlevaninezhad, A. M. Lee, S. Lam, C. MacAulay, and P. M. Lane, “Coregistered autofluorescence-optical coherence tomography imaging of human lung sections,” J. Biomed. Opt. 19(3), 036022 (2014).
[Crossref] [PubMed]

2013 (4)

2012 (4)

N. Iftimia, A. K. Iyer, D. X. Hammer, N. Lue, M. Mujat, M. Pitman, R. D. Ferguson, and M. Amiji, “Fluorescence-guided optical coherence tomography imaging for colon cancer screening: a preliminary mouse study,” Biomed. Opt. Express 3(1), 178–191 (2012).
[Crossref] [PubMed]

E. Carreño, A. Portero, J. M. Herreras, and M. I. López, “Assesment of fundus autofluorescence in serpiginous and serpiginous-like choroidopathy,” Eye (Lond.) 26(9), 1232–1236 (2012).
[Crossref] [PubMed]

B. W. Graf and S. A. Boppart, “Multimodal in vivo skin imaging with integrated optical coherence and multiphoton microscopy,” IEEE J. Sel. Top. Quantum Electron. 18(4), 1280–1286 (2012).
[Crossref]

C. Dai, X. Liu, and S. Jiao, “Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source,” J. Biomed. Opt. 17(8), 080502 (2012).
[Crossref] [PubMed]

2011 (2)

2010 (4)

J. Park, J. A. Jo, S. Shrestha, P. Pande, Q. Wan, and B. E. Applegate, “A dual-modality optical coherence tomography and fluorescence lifetime imaging microscopy system for simultaneous morphological and biochemical tissue characterization,” Biomed. Opt. Express 1(1), 186–200 (2010).
[Crossref] [PubMed]

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

A. D. Singh, R. N. Belfort, K. Sayanagi, and P. K. Kaiser, “Fourier domain optical coherence tomographic and auto-fluorescence findings in indeterminate choroidal melanocytic lesions,” Br. J. Ophthalmol. 94(4), 474–478 (2010).
[Crossref] [PubMed]

D. F. Kiernan, W. F. Mieler, and S. M. Hariprasad, “Spectral-domain optical coherence tomography: a comparison of modern high-resolution retinal imaging systems,” Am. J. Ophthalmol. 149(1), 18–31 (2010).
[Crossref] [PubMed]

2009 (2)

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

A. Bradu, L. Ma, J. W. Bloor, and A. Podoleanu, “Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart,” J. Biophotonics 2(6-7), 380–388 (2009).
[Crossref] [PubMed]

2008 (1)

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

2006 (3)

D. C. Gray, W. Merigan, J. I. Wolfing, B. P. Gee, J. Porter, A. Dubra, T. H. Twietmeyer, K. Ahamd, R. Tumbar, F. Reinholz, and D. R. Williams, “In vivo fluorescence imaging of primate retinal ganglion cells and retinal pigment epithelial cells,” Opt. Express 14(16), 7144–7158 (2006).
[Crossref] [PubMed]

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, “Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source,” J. Biomed. Opt. 11(2), 020502 (2006).
[Crossref] [PubMed]

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

2005 (3)

Z. G. Wang, D. B. Durand, M. Schoenberg, and Y. T. Pan, “Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model,” J. Urol. 174(6), 2376–2381 (2005).
[Crossref] [PubMed]

J. Dolar-Szczasny, J. Mackiewicz, A. Bindewald, F. G. Holz, and Z. Zagórski, “Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina Angiograph),” Klin. Oczna 107(7-9), 544–547 (2005).
[PubMed]

S. Jiao, R. Knighton, X. Huang, G. Gregori, and C. Puliafito, “Simultaneous acquisition of sectional and fundus ophthalmic images with spectral-domain optical coherence tomography,” Opt. Express 13(2), 444–452 (2005).
[Crossref] [PubMed]

2002 (2)

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
[Crossref]

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

2000 (1)

N. Ramanujam, “Fluorescence spectroscopy of neoplastic and non-neoplastic tissues,” Neoplasia 2(1-2), 89–117 (2000).
[Crossref] [PubMed]

1996 (1)

A. F. Fercher, “Optical coherence tomography,” J. Biomed. Opt. 1(2), 157–173 (1996).
[Crossref] [PubMed]

1995 (1)

C. J. Kennedy, P. E. Rakoczy, and I. J. Constable, “Lipofuscin of the retinal pigment epithelium: a review,” Eye (Lond.) 9(6), 763–771 (1995).
[Crossref] [PubMed]

1991 (1)

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref] [PubMed]

1985 (1)

D. Grotte, V. Mattox, and R. Brubaker, “Fluorescent, physiological and pharmacokinetic properties of fluorescein glucuronide,” Exp. Eye Res. 40(1), 23–33 (1985).
[Crossref] [PubMed]

Ahamd, K.

Ahlers, C.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Amiji, M.

Apolonski, A. A.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Applegate, B. E.

Auger, M.

Backman, V.

Barton, J. K.

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

Belfort, R. N.

A. D. Singh, R. N. Belfort, K. Sayanagi, and P. K. Kaiser, “Fourier domain optical coherence tomographic and auto-fluorescence findings in indeterminate choroidal melanocytic lesions,” Br. J. Ophthalmol. 94(4), 474–478 (2010).
[Crossref] [PubMed]

Besselsen, D. G.

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

Bindewald, A.

J. Dolar-Szczasny, J. Mackiewicz, A. Bindewald, F. G. Holz, and Z. Zagórski, “Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina Angiograph),” Klin. Oczna 107(7-9), 544–547 (2005).
[PubMed]

Bizheva, K. K.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Bloor, J. W.

A. Bradu, L. Ma, J. W. Bloor, and A. Podoleanu, “Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart,” J. Biophotonics 2(6-7), 380–388 (2009).
[Crossref] [PubMed]

Booysen, D. J.

D. J. Booysen, “A review of fundus autofluorescence imaging,” S Afr Optom 72, 46–53 (2013).

Boppart, S. A.

B. W. Graf and S. A. Boppart, “Multimodal in vivo skin imaging with integrated optical coherence and multiphoton microscopy,” IEEE J. Sel. Top. Quantum Electron. 18(4), 1280–1286 (2012).
[Crossref]

Boudoux, C.

Bradu, A.

A. Bradu, L. Ma, J. W. Bloor, and A. Podoleanu, “Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart,” J. Biophotonics 2(6-7), 380–388 (2009).
[Crossref] [PubMed]

Brubaker, R.

D. Grotte, V. Mattox, and R. Brubaker, “Fluorescent, physiological and pharmacokinetic properties of fluorescein glucuronide,” Exp. Eye Res. 40(1), 23–33 (1985).
[Crossref] [PubMed]

Cable, A.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Carreño, E.

E. Carreño, A. Portero, J. M. Herreras, and M. I. López, “Assesment of fundus autofluorescence in serpiginous and serpiginous-like choroidopathy,” Eye (Lond.) 26(9), 1232–1236 (2012).
[Crossref] [PubMed]

Chang, W.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Chen, C.-W.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Chen, M.

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

Chen, Y.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Chen, Z.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, “Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source,” J. Biomed. Opt. 11(2), 020502 (2006).
[Crossref] [PubMed]

Constable, I. J.

C. J. Kennedy, P. E. Rakoczy, and I. J. Constable, “Lipofuscin of the retinal pigment epithelium: a review,” Eye (Lond.) 9(6), 763–771 (1995).
[Crossref] [PubMed]

Dai, C.

C. Dai, X. Liu, and S. Jiao, “Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source,” J. Biomed. Opt. 17(8), 080502 (2012).
[Crossref] [PubMed]

Deak, G.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref] [PubMed]

Dolar-Szczasny, J.

J. Dolar-Szczasny, J. Mackiewicz, A. Bindewald, F. G. Holz, and Z. Zagórski, “Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina Angiograph),” Klin. Oczna 107(7-9), 544–547 (2005).
[PubMed]

Drexler, W.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Dubra, A.

Durand, D. B.

Z. G. Wang, D. B. Durand, M. Schoenberg, and Y. T. Pan, “Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model,” J. Urol. 174(6), 2376–2381 (2005).
[Crossref] [PubMed]

Fercher, A. F.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

A. F. Fercher, “Optical coherence tomography,” J. Biomed. Opt. 1(2), 157–173 (1996).
[Crossref] [PubMed]

Ferguson, R. D.

Flotte, T.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Forrester, J. V.

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

Gee, B. P.

Gelikonov, V. M.

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
[Crossref]

Gerner, E. W.

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

Godbout, N.

Golbaz, I.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
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Graf, B. W.

B. W. Graf and S. A. Boppart, “Multimodal in vivo skin imaging with integrated optical coherence and multiphoton microscopy,” IEEE J. Sel. Top. Quantum Electron. 18(4), 1280–1286 (2012).
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Grant, G.

F. E. Robles, C. Wilson, G. Grant, and A. Wax, “Molecular imaging true-colour spectroscopic optical coherence tomography,” Nat. Photonics 5(12), 744–747 (2011).
[Crossref] [PubMed]

Gray, D. C.

Gregori, G.

Gregory, K.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Griffiths, G.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
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D. Grotte, V. Mattox, and R. Brubaker, “Fluorescent, physiological and pharmacokinetic properties of fluorescein glucuronide,” Exp. Eye Res. 40(1), 23–33 (1985).
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Hammer, D. X.

Hariprasad, S. M.

D. F. Kiernan, W. F. Mieler, and S. M. Hariprasad, “Spectral-domain optical coherence tomography: a comparison of modern high-resolution retinal imaging systems,” Am. J. Ophthalmol. 149(1), 18–31 (2010).
[Crossref] [PubMed]

Hariri, L. P.

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

Hee, M. R.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Hermann, B.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
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Herreras, J. M.

E. Carreño, A. Portero, J. M. Herreras, and M. I. López, “Assesment of fundus autofluorescence in serpiginous and serpiginous-like choroidopathy,” Eye (Lond.) 26(9), 1232–1236 (2012).
[Crossref] [PubMed]

Holz, F. G.

J. Dolar-Szczasny, J. Mackiewicz, A. Bindewald, F. G. Holz, and Z. Zagórski, “Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina Angiograph),” Klin. Oczna 107(7-9), 544–547 (2005).
[PubMed]

Hu, J.

Huang, D.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Huang, X.

Huang, X.-R.

Iftimia, N.

Iyer, A. K.

Jiang, J.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Jiao, S.

Jo, J. A.

Kaiser, P. K.

A. D. Singh, R. N. Belfort, K. Sayanagi, and P. K. Kaiser, “Fourier domain optical coherence tomographic and auto-fluorescence findings in indeterminate choroidal melanocytic lesions,” Br. J. Ophthalmol. 94(4), 474–478 (2010).
[Crossref] [PubMed]

Kennedy, C. J.

C. J. Kennedy, P. E. Rakoczy, and I. J. Constable, “Lipofuscin of the retinal pigment epithelium: a review,” Eye (Lond.) 9(6), 763–771 (1995).
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Kiernan, D. F.

D. F. Kiernan, W. F. Mieler, and S. M. Hariprasad, “Spectral-domain optical coherence tomography: a comparison of modern high-resolution retinal imaging systems,” Am. J. Ophthalmol. 149(1), 18–31 (2010).
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Kirk, R. W.

Knighton, R.

Knighton, R. W.

Komar, K.

Krasieva, T. B.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, “Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source,” J. Biomed. Opt. 11(2), 020502 (2006).
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Krausz, F.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
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Kuranov, R. V.

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
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Lam, S.

H. Pahlevaninezhad, A. M. Lee, S. Lam, C. MacAulay, and P. M. Lane, “Coregistered autofluorescence-optical coherence tomography imaging of human lung sections,” J. Biomed. Opt. 19(3), 036022 (2014).
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Lane, P. M.

H. Pahlevaninezhad, A. M. Lee, S. Lam, C. MacAulay, and P. M. Lane, “Coregistered autofluorescence-optical coherence tomography imaging of human lung sections,” J. Biomed. Opt. 19(3), 036022 (2014).
[Crossref] [PubMed]

Lee, A. M.

H. Pahlevaninezhad, A. M. Lee, S. Lam, C. MacAulay, and P. M. Lane, “Coregistered autofluorescence-optical coherence tomography imaging of human lung sections,” J. Biomed. Opt. 19(3), 036022 (2014).
[Crossref] [PubMed]

Lin, C. P.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Liu, W.

Liu, X.

C. Dai, X. Liu, and S. Jiao, “Simultaneous optical coherence tomography and autofluorescence microscopy with a single light source,” J. Biomed. Opt. 17(8), 080502 (2012).
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Lois, N.

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

López, M. I.

E. Carreño, A. Portero, J. M. Herreras, and M. I. López, “Assesment of fundus autofluorescence in serpiginous and serpiginous-like choroidopathy,” Eye (Lond.) 26(9), 1232–1236 (2012).
[Crossref] [PubMed]

Lorenser, D.

Lue, N.

Ma, H.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Ma, L.

A. Bradu, L. Ma, J. W. Bloor, and A. Podoleanu, “Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart,” J. Biophotonics 2(6-7), 380–388 (2009).
[Crossref] [PubMed]

MacAulay, C.

H. Pahlevaninezhad, A. M. Lee, S. Lam, C. MacAulay, and P. M. Lane, “Coregistered autofluorescence-optical coherence tomography imaging of human lung sections,” J. Biomed. Opt. 19(3), 036022 (2014).
[Crossref] [PubMed]

Mackiewicz, J.

J. Dolar-Szczasny, J. Mackiewicz, A. Bindewald, F. G. Holz, and Z. Zagórski, “Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina Angiograph),” Klin. Oczna 107(7-9), 544–547 (2005).
[PubMed]

Madore, W.-J.

Malamos, P.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Manivannan, A.

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

Mattox, V.

D. Grotte, V. Mattox, and R. Brubaker, “Fluorescent, physiological and pharmacokinetic properties of fluorescein glucuronide,” Exp. Eye Res. 40(1), 23–33 (1985).
[Crossref] [PubMed]

McLaughlin, R. A.

Merigan, W.

Mieler, W. F.

D. F. Kiernan, W. F. Mieler, and S. M. Hariprasad, “Spectral-domain optical coherence tomography: a comparison of modern high-resolution retinal imaging systems,” Am. J. Ophthalmol. 149(1), 18–31 (2010).
[Crossref] [PubMed]

Motoczynska, M.

Mujat, M.

Mylonas, G.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Pahlevaninezhad, H.

H. Pahlevaninezhad, A. M. Lee, S. Lam, C. MacAulay, and P. M. Lane, “Coregistered autofluorescence-optical coherence tomography imaging of human lung sections,” J. Biomed. Opt. 19(3), 036022 (2014).
[Crossref] [PubMed]

Pan, Y. T.

Z. G. Wang, D. B. Durand, M. Schoenberg, and Y. T. Pan, “Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model,” J. Urol. 174(6), 2376–2381 (2005).
[Crossref] [PubMed]

Pande, P.

Park, J.

Petrova, S. A.

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
[Crossref]

Pitman, M.

Podoleanu, A.

A. Bradu, L. Ma, J. W. Bloor, and A. Podoleanu, “Dual optical coherence tomography/fluorescence microscopy for monitoring of Drosophila melanogaster larval heart,” J. Biophotonics 2(6-7), 380–388 (2009).
[Crossref] [PubMed]

Porter, J.

Portero, A.

E. Carreño, A. Portero, J. M. Herreras, and M. I. López, “Assesment of fundus autofluorescence in serpiginous and serpiginous-like choroidopathy,” Eye (Lond.) 26(9), 1232–1236 (2012).
[Crossref] [PubMed]

Povazay, B.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Puliafito, C.

Puliafito, C. A.

X. Zhang, J. Hu, R. W. Knighton, X.-R. Huang, C. A. Puliafito, and S. Jiao, “Dual-band spectral-domain optical coherence tomography for in vivo imaging the spectral contrasts of the retinal nerve fiber layer,” Opt. Express 19(20), 19653–19659 (2011).
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D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Quirk, B. C.

Rakoczy, P. E.

C. J. Kennedy, P. E. Rakoczy, and I. J. Constable, “Lipofuscin of the retinal pigment epithelium: a review,” Eye (Lond.) 9(6), 763–771 (1995).
[Crossref] [PubMed]

Ramanujam, N.

N. Ramanujam, “Fluorescence spectroscopy of neoplastic and non-neoplastic tissues,” Neoplasia 2(1-2), 89–117 (2000).
[Crossref] [PubMed]

Reinholz, F.

Robles, F. E.

F. E. Robles, C. Wilson, G. Grant, and A. Wax, “Molecular imaging true-colour spectroscopic optical coherence tomography,” Nat. Photonics 5(12), 744–747 (2011).
[Crossref] [PubMed]

Roney, C. A.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Russell, P. S. J.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Sacu, S.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Sampson, D. D.

Sapozhnikova, V. V. e.

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
[Crossref]

Sattmann, H.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Sayanagi, K.

A. D. Singh, R. N. Belfort, K. Sayanagi, and P. K. Kaiser, “Fourier domain optical coherence tomographic and auto-fluorescence findings in indeterminate choroidal melanocytic lesions,” Br. J. Ophthalmol. 94(4), 474–478 (2010).
[Crossref] [PubMed]

Schmidt-Erfurth, U.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Schoenberg, M.

Z. G. Wang, D. B. Durand, M. Schoenberg, and Y. T. Pan, “Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model,” J. Urol. 174(6), 2376–2381 (2005).
[Crossref] [PubMed]

Schuetze, C.

G. Mylonas, C. Ahlers, P. Malamos, I. Golbaz, G. Deak, C. Schuetze, S. Sacu, and U. Schmidt-Erfurth, “Comparison of retinal thickness measurements and segmentation performance of four different spectral and time domain OCT devices in neovascular age-related macular degeneration,” Br. J. Ophthalmol. 93(11), 1453–1460 (2009).
[Crossref] [PubMed]

Schuman, J. S.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Shakhova, N. M.

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
[Crossref]

Shrestha, S.

Singh, A. D.

A. D. Singh, R. N. Belfort, K. Sayanagi, and P. K. Kaiser, “Fourier domain optical coherence tomographic and auto-fluorescence findings in indeterminate choroidal melanocytic lesions,” Br. J. Ophthalmol. 94(4), 474–478 (2010).
[Crossref] [PubMed]

Stinson, W. G.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Stremplewski, P.

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref] [PubMed]

Summers, R. M.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Swanson, E. A.

D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, and C. A. Puliafito, “Optical coherence tomography,” Science 254(5035), 1178–1181 (1991).
[Crossref] [PubMed]

Szkulmowski, M.

Tang, S.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, “Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source,” J. Biomed. Opt. 11(2), 020502 (2006).
[Crossref] [PubMed]

Tromberg, B. J.

S. Tang, T. B. Krasieva, Z. Chen, and B. J. Tromberg, “Combined multiphoton microscopy and optical coherence tomography using a 12-fs broadband source,” J. Biomed. Opt. 11(2), 020502 (2006).
[Crossref] [PubMed]

Tumbar, R.

Tumlinson, A. R.

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

Twietmeyer, T. H.

Unterhuber, A.

B. Povazay, A. A. Apolonski, A. Unterhuber, B. Hermann, K. K. Bizheva, H. Sattmann, P. S. J. Russell, F. Krausz, A. F. Fercher, and W. Drexler, “Visible light optical coherence tomography,” Proc. SPIE 4619, 90–94 (2002).
[Crossref]

Utzinger, U.

L. P. Hariri, A. R. Tumlinson, D. G. Besselsen, U. Utzinger, E. W. Gerner, and J. K. Barton, “Endoscopic optical coherence tomography and laser-induced fluorescence spectroscopy in a murine colon cancer model,” Lasers Surg. Med. 38(4), 305–313 (2006).
[Crossref] [PubMed]

Wan, Q.

Wang, Z. G.

Z. G. Wang, D. B. Durand, M. Schoenberg, and Y. T. Pan, “Fluorescence guided optical coherence tomography for the diagnosis of early bladder cancer in a rat model,” J. Urol. 174(6), 2376–2381 (2005).
[Crossref] [PubMed]

Wax, A.

F. E. Robles, C. Wilson, G. Grant, and A. Wax, “Molecular imaging true-colour spectroscopic optical coherence tomography,” Nat. Photonics 5(12), 744–747 (2011).
[Crossref] [PubMed]

Webb, W. W.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref] [PubMed]

Wei, Q.

Wierwille, J.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Williams, D. R.

Wilson, C.

F. E. Robles, C. Wilson, G. Grant, and A. Wax, “Molecular imaging true-colour spectroscopic optical coherence tomography,” Nat. Photonics 5(12), 744–747 (2011).
[Crossref] [PubMed]

Wojtkowski, M.

Wolfing, J. I.

Xu, B.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Xu, H.

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

Yi, J.

Yuan, S.

S. Yuan, C. A. Roney, J. Wierwille, C.-W. Chen, B. Xu, G. Griffiths, J. Jiang, H. Ma, A. Cable, R. M. Summers, and Y. Chen, “Co-registered optical coherence tomography and fluorescence molecular imaging for simultaneous morphological and molecular imaging,” Phys. Med. Biol. 55(1), 191–206 (2010).
[Crossref] [PubMed]

Zagainova, E. V.

R. V. Kuranov, V. V. e. Sapozhnikova, N. M. Shakhova, V. M. Gelikonov, E. V. Zagainova, and S. A. Petrova, “Combined application of optical methods to increase the information content of optical coherent tomography in diagnostics of neoplastic processes,” Quantum Electron. 32(11), 993–998 (2002).
[Crossref]

Zagórski, Z.

J. Dolar-Szczasny, J. Mackiewicz, A. Bindewald, F. G. Holz, and Z. Zagórski, “Fundus autofluorescence examination using a confocal scanning laser ophthalmoscope HRA (Heidelberg Retina Angiograph),” Klin. Oczna 107(7-9), 544–547 (2005).
[PubMed]

Zhang, H. F.

Zhang, X.

Aging Cell (1)

H. Xu, M. Chen, A. Manivannan, N. Lois, and J. V. Forrester, “Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice,” Aging Cell 7(1), 58–68 (2008).
[Crossref] [PubMed]

Am. J. Ophthalmol. (1)

D. F. Kiernan, W. F. Mieler, and S. M. Hariprasad, “Spectral-domain optical coherence tomography: a comparison of modern high-resolution retinal imaging systems,” Am. J. Ophthalmol. 149(1), 18–31 (2010).
[Crossref] [PubMed]

Biomed. Opt. Express (3)

Br. J. Ophthalmol. (2)

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Figures (5)

Fig. 1
Fig. 1 (a) Schematic of the experimental system. C1-C3: Collimator; L1-L3: Lens; DM: Dichroic mirror; LPF: Long-pass filter; PC: Polarization controller; PMT: Photomultiplier; (b) The measured spectrum of selected output from the light source.
Fig. 2
Fig. 2 VIS-OCT cross-sectional image of a rat retina consisting of 2048 depth scans. Bar: 200 μm.
Fig. 3
Fig. 3 VIS-OCT and AF images simultaneously acquired from a rat retina in vivo. (a) OCT fundus image; (b) AF image; (c) OCT B-scan image. Bar: 200 μm. The white line in the OCT fundus image marks the location of the OCT B-scan image.
Fig. 4
Fig. 4 OCT fundus images (a, d, g), AF images (b, e, h) and histograms of the AF intensity distributions (c, f, i) of one rat at different ages: (a) – (c) 10 weeks, (d) – (f) 12 weeks, (g)-(i) 14 weeks. Numbers 1, 2 and 3 mark the corresponding vessels at different time point.
Fig. 5
Fig. 5 The mean AF intensity plus standard deviation of the 3 rats at different ages.

Tables (1)

Tables Icon

Table 1 The mean intensities of both the OCT and AF images of the rat retina shown in Fig. 4

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