Abstract

Light-sheet imaging is rapidly gaining importance for imaging intact biological specimens. Many of the latest innovations rely on the propagation-invariant Bessel or Airy beams to form an extended light sheet to provide high resolution across a large field of view. Shaping light to realize propagation-invariant beams often relies on complex programming of spatial light modulators or specialized, custom made, optical elements. Here we present a straightforward and low-cost modification to the traditional light-sheet setup, based on the open-access light-sheet microscope OpenSPIM, to achieve Airy light-sheet illumination. This brings wide field single-photon light-sheet imaging to a broader range of endusers. Fluorescent microspheres embedded in agarose and a zebrafish larva were imaged to demonstrate how such a microscope can have a minimal footprint and cost without compromising on imaging quality.

© 2014 Optical Society of America

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2014 (2)

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, B.-C. Chen, and E. Betzig, “3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy,” Nat. Protoc. 9, 1083–1101 (2014).
[Crossref] [PubMed]

2013 (1)

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

2012 (3)

R. Tomer, K. Khairy, F. Amat, and P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9, 755–763 (2012).
[Crossref] [PubMed]

O. E. Olarte, J. Licea-Rodriguez, J. A. Palero, E. J. Gualda, D. Artigas, J. Mayer, J. Swoger, J. Sharpe, I. RochaMendoza, R. Rangel-Rojo, and P. Loza-Alvarez, “Image formation by linear and nonlinear digital scanned lightsheet fluorescence microscopy with Gaussian and Bessel beam profiles,” Biomed. Opt. Express 3(7), 1492–1505 (2012).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

2011 (2)

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

J. Wang, J. Bu, M. Wang, Y. Yang, and X. Yuan, “Generation of high quality airy beams with blazed micro-optical cubic phase plates,” Appl. Opt. 50(36), 6627–6631 (2011).
[Crossref] [PubMed]

2010 (1)

D. G. Papazoglou, S. Suntsov, D. Abdollahpour, and S. Tzortzakis, “Tunable intense Airy beams and tailored femtosecond laser filaments,” Phys. Rev. A 81, 061807 (2010).
[Crossref]

2009 (2)

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

P. Polynkin, M. Kolesik, and J. Moloney, “Filamentation of femtosecond laser Airy beams in water,” Phys. Rev. Lett. 103, 123902 (2009).
[Crossref] [PubMed]

2008 (4)

J. Baumgartl, M. Mazilu, and K. Dholakia, “Optically mediated particle clearing using Airy wavepackets,” Nat. Photon. 2, 675–678 (2008).
[Crossref]

H. Zhao, Q. Li, and H. Feng, “Improved logarithmic phase mask to extend the depth of field of an incoherent imaging system,” Opt. Lett. 33(11), 1171–1173 (2008).
[Crossref] [PubMed]

J. Broky, G. A. Siviloglou, A. Dogariu, and D. N. Christodoulides, “Self-healing properties of optical Airy beams,” Opt. Express 16(17), 12880–12891 (2008).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref] [PubMed]

2007 (3)

G. A. Siviloglou, J. Broky, A. Dogariu, and D. N. Christodoulides, “Observation of accelerating Airy beams,” Phys. Rev. Lett. 99, 213901 (2007).
[Crossref]

J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007).
[Crossref] [PubMed]

J. A. N. Buytaert and J. J. J. Dirckx, “Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolution,” J. Biomed. Opt. 12(1), 014039 (2007).
[Crossref] [PubMed]

2006 (2)

2004 (1)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

2003 (1)

2001 (1)

Abdollahpour, D.

D. G. Papazoglou, S. Suntsov, D. Abdollahpour, and S. Tzortzakis, “Tunable intense Airy beams and tailored femtosecond laser filaments,” Phys. Rev. A 81, 061807 (2010).
[Crossref]

Amat, F.

R. Tomer, K. Khairy, F. Amat, and P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9, 755–763 (2012).
[Crossref] [PubMed]

Artigas, D.

Baumgartl, J.

J. Baumgartl, M. Mazilu, and K. Dholakia, “Optically mediated particle clearing using Airy wavepackets,” Nat. Photon. 2, 675–678 (2008).
[Crossref]

Betzig, E.

L. Gao, L. Shao, B.-C. Chen, and E. Betzig, “3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy,” Nat. Protoc. 9, 1083–1101 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Broky, J.

J. Broky, G. A. Siviloglou, A. Dogariu, and D. N. Christodoulides, “Self-healing properties of optical Airy beams,” Opt. Express 16(17), 12880–12891 (2008).
[Crossref] [PubMed]

G. A. Siviloglou, J. Broky, A. Dogariu, and D. N. Christodoulides, “Observation of accelerating Airy beams,” Phys. Rev. Lett. 99, 213901 (2007).
[Crossref]

Bu, J.

Buytaert, J. A. N.

J. A. N. Buytaert and J. J. J. Dirckx, “Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolution,” J. Biomed. Opt. 12(1), 014039 (2007).
[Crossref] [PubMed]

Chen, B.-C.

L. Gao, L. Shao, B.-C. Chen, and E. Betzig, “3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy,” Nat. Protoc. 9, 1083–1101 (2014).
[Crossref] [PubMed]

Chi, W.

Christodoulides, D. N.

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

J. Broky, G. A. Siviloglou, A. Dogariu, and D. N. Christodoulides, “Self-healing properties of optical Airy beams,” Opt. Express 16(17), 12880–12891 (2008).
[Crossref] [PubMed]

G. A. Siviloglou, J. Broky, A. Dogariu, and D. N. Christodoulides, “Observation of accelerating Airy beams,” Phys. Rev. Lett. 99, 213901 (2007).
[Crossref]

Cižmár, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Coll-Lladó, C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Dalgarno, H. I. C.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Davidson, M. W.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Dholakia, K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

J. Baumgartl, M. Mazilu, and K. Dholakia, “Optically mediated particle clearing using Airy wavepackets,” Nat. Photon. 2, 675–678 (2008).
[Crossref]

Dirckx, J. J. J.

J. A. N. Buytaert and J. J. J. Dirckx, “Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolution,” J. Biomed. Opt. 12(1), 014039 (2007).
[Crossref] [PubMed]

Dogariu, A.

J. Broky, G. A. Siviloglou, A. Dogariu, and D. N. Christodoulides, “Self-healing properties of optical Airy beams,” Opt. Express 16(17), 12880–12891 (2008).
[Crossref] [PubMed]

G. A. Siviloglou, J. Broky, A. Dogariu, and D. N. Christodoulides, “Observation of accelerating Airy beams,” Phys. Rev. Lett. 99, 213901 (2007).
[Crossref]

Eliceiri, K. W.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Engelbrecht, C. J.

Feng, H.

Ferrier, D. E. K.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Galbraith, C. G.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Galbraith, J. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Gao, L.

L. Gao, L. Shao, B.-C. Chen, and E. Betzig, “3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy,” Nat. Protoc. 9, 1083–1101 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

George, N.

Goldstein, B.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

Gonzalez, R. C.

R. C. Gonzalez and R. E. Woods, Digital Image Processing, 3rd ed. (Prentice Hall, 2007).

Gualda, E. J.

Gunn-Moore, F. J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Harvey, A. R.

Higgins, C. D.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

Huisken, J.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

J. Huisken and D. Y. R. Stainier, “Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM),” Opt. Lett. 32(17), 2608–2610 (2007).
[Crossref] [PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Keller, P. J.

R. Tomer, K. Khairy, F. Amat, and P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9, 755–763 (2012).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref] [PubMed]

Khairy, K.

R. Tomer, K. Khairy, F. Amat, and P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9, 755–763 (2012).
[Crossref] [PubMed]

Kolesik, M.

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

P. Polynkin, M. Kolesik, and J. Moloney, “Filamentation of femtosecond laser Airy beams in water,” Phys. Rev. Lett. 103, 123902 (2009).
[Crossref] [PubMed]

Li, Q.

Licea-Rodriguez, J.

Loza-Alvarez, P.

Mayer, J.

Mazilu, M.

J. Baumgartl, M. Mazilu, and K. Dholakia, “Optically mediated particle clearing using Airy wavepackets,” Nat. Photon. 2, 675–678 (2008).
[Crossref]

Mezouari, S.

Milkie, D. E.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Moloney, J.

P. Polynkin, M. Kolesik, and J. Moloney, “Filamentation of femtosecond laser Airy beams in water,” Phys. Rev. Lett. 103, 123902 (2009).
[Crossref] [PubMed]

Moloney, J. V.

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

Muyo, G.

Nylk, J.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Olarte, O. E.

Palero, J. A.

Papazoglou, D. G.

D. G. Papazoglou, S. Suntsov, D. Abdollahpour, and S. Tzortzakis, “Tunable intense Airy beams and tailored femtosecond laser filaments,” Phys. Rev. A 81, 061807 (2010).
[Crossref]

Peifer, M.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

Pitrone, P. G.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Planchon, T. A.

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

Polynkin, P.

P. Polynkin, M. Kolesik, and J. Moloney, “Filamentation of femtosecond laser Airy beams in water,” Phys. Rev. Lett. 103, 123902 (2009).
[Crossref] [PubMed]

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

Poulton, J. S.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

Preibisch, S.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Rangel-Rojo, R.

RochaMendoza, I.

Schindelin, J.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Schmidt, A. D.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref] [PubMed]

Shao, L.

L. Gao, L. Shao, B.-C. Chen, and E. Betzig, “3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy,” Nat. Protoc. 9, 1083–1101 (2014).
[Crossref] [PubMed]

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

Sharpe, J.

Siviloglou, G. A.

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

J. Broky, G. A. Siviloglou, A. Dogariu, and D. N. Christodoulides, “Self-healing properties of optical Airy beams,” Opt. Express 16(17), 12880–12891 (2008).
[Crossref] [PubMed]

G. A. Siviloglou, J. Broky, A. Dogariu, and D. N. Christodoulides, “Observation of accelerating Airy beams,” Phys. Rev. Lett. 99, 213901 (2007).
[Crossref]

Stainier, D. Y. R.

Stelzer, E. H. K.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref] [PubMed]

C. J. Engelbrecht and E. H. K. Stelzer, “Resolution enhancement in a light-sheet-based microscope (SPIM),” Opt. Lett. 31(10), 1477–1479 (2006).
[Crossref] [PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Stuyvenberg, L.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Suntsov, S.

D. G. Papazoglou, S. Suntsov, D. Abdollahpour, and S. Tzortzakis, “Tunable intense Airy beams and tailored femtosecond laser filaments,” Phys. Rev. A 81, 061807 (2010).
[Crossref]

Swoger, J.

Tomancak, P.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Tomer, R.

R. Tomer, K. Khairy, F. Amat, and P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9, 755–763 (2012).
[Crossref] [PubMed]

Tzortzakis, S.

D. G. Papazoglou, S. Suntsov, D. Abdollahpour, and S. Tzortzakis, “Tunable intense Airy beams and tailored femtosecond laser filaments,” Phys. Rev. A 81, 061807 (2010).
[Crossref]

Vettenburg, T.

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

Wang, J.

Wang, M.

Weber, M.

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

Wittbrodt, J.

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref] [PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

Woods, R. E.

R. C. Gonzalez and R. E. Woods, Digital Image Processing, 3rd ed. (Prentice Hall, 2007).

Wu, X.

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

Yang, Y.

Yuan, X.

Zhao, H.

Appl. Opt. (1)

Biomed. Opt. Express (1)

Cell (1)

L. Gao, L. Shao, C. D. Higgins, J. S. Poulton, M. Peifer, M. W. Davidson, X. Wu, B. Goldstein, and E. Betzig, “Noninvasive imaging beyond the diffraction limit of 3D dynamics in thickly fluorescent specimens,” Cell 151(6), 1370–1385 (2012).
[Crossref] [PubMed]

J. Biomed. Opt. (1)

J. A. N. Buytaert and J. J. J. Dirckx, “Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolution,” J. Biomed. Opt. 12(1), 014039 (2007).
[Crossref] [PubMed]

J. Opt. Soc. Am. A (1)

Nat. Methods (4)

T. A. Planchon, L. Gao, D. E. Milkie, M. W. Davidson, J. A. Galbraith, C. G. Galbraith, and E. Betzig, “Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination,” Nat. Methods 8, 417–423 (2011).
[Crossref] [PubMed]

P. G. Pitrone, J. Schindelin, L. Stuyvenberg, S. Preibisch, M. Weber, K. W. Eliceiri, J. Huisken, and P. Tomancak, “OpenSPIM: an open-access light-sheet microscopy platform,” Nat. Methods 10, 598–599 (2013).
[Crossref] [PubMed]

T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Lladó, D. E. K. Ferrier, T. Čižmár, F. J. Gunn-Moore, and K. Dholakia, “Light-sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).
[Crossref] [PubMed]

R. Tomer, K. Khairy, F. Amat, and P. J. Keller, “Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy,” Nat. Methods 9, 755–763 (2012).
[Crossref] [PubMed]

Nat. Photon. (1)

J. Baumgartl, M. Mazilu, and K. Dholakia, “Optically mediated particle clearing using Airy wavepackets,” Nat. Photon. 2, 675–678 (2008).
[Crossref]

Nat. Protoc. (1)

L. Gao, L. Shao, B.-C. Chen, and E. Betzig, “3D live fluorescence imaging of cellular dynamics using Bessel beam plane illumination microscopy,” Nat. Protoc. 9, 1083–1101 (2014).
[Crossref] [PubMed]

Opt. Express (1)

Opt. Lett. (5)

Phys. Rev. A (1)

D. G. Papazoglou, S. Suntsov, D. Abdollahpour, and S. Tzortzakis, “Tunable intense Airy beams and tailored femtosecond laser filaments,” Phys. Rev. A 81, 061807 (2010).
[Crossref]

Phys. Rev. Lett. (2)

P. Polynkin, M. Kolesik, and J. Moloney, “Filamentation of femtosecond laser Airy beams in water,” Phys. Rev. Lett. 103, 123902 (2009).
[Crossref] [PubMed]

G. A. Siviloglou, J. Broky, A. Dogariu, and D. N. Christodoulides, “Observation of accelerating Airy beams,” Phys. Rev. Lett. 99, 213901 (2007).
[Crossref]

Science (3)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305(5686), 1007–1009 (2004).
[Crossref] [PubMed]

P. Polynkin, M. Kolesik, J. V. Moloney, G. A. Siviloglou, and D. N. Christodoulides, “Curved plasma channel generation using ultraintense Airy beams,” Science 324(5924), 229–232 (2009).
[Crossref] [PubMed]

P. J. Keller, A. D. Schmidt, J. Wittbrodt, and E. H. K. Stelzer, “Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy,” Science 322(5904), 1065–1069 (2008).
[Crossref] [PubMed]

Other (1)

R. C. Gonzalez and R. E. Woods, Digital Image Processing, 3rd ed. (Prentice Hall, 2007).

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Figures (4)

Fig. 1
Fig. 1

Schematic of the open-access Airy light-sheet microscope. (a) Experimental setup with tilted cylindrical lens (CL). (b) shows the projection of measured Airy light sheet. (c) The red line shows the beam profile of a cross section on (b). The blue line indicates the corresponding Airy beam profile from fitted model.

Fig. 2
Fig. 2

Influence of the cylindrical lens tilt angle. (a) focal length, (b) optical axis displacement, (c) cubic and, (d) higher order modulation residual, as a function of the lens angle. The line colors correspond to the wavelengths 405nm, 488nm, 532nm, 561nm, and 633nm. At the wavelength of 532nm used in the experiments, the α value is 1.21 at 35°, 4.13 at 40°, and 11.24 at 45°, covering the values useful for Airy beam light sheet microscopy. Almost no higher order terms are present as can be seen from (d). The residual phase modulation has a standard deviation of only 0.026λ, 0.006λ, and 0.061λ, respectively. The corresponding focal lengths are 26mm, 22mm, and 18mm. The axis position shifts by 1.6mm, 1.9mm, and, 2.3mm. The focus position at a wavelength of 488nm differs by less than 1%. As a result, the optics do not need to be adjusted for minor changes in wavelength.

Fig. 3
Fig. 3

Vertical projection of a sample with fluorescent microspheres (∅ = 600nm), before deconvolution (a), and after deconvolution (b). Although before deconvolution the fluorescent microspheres appear blurred in the axial dimension, z, the pattern is relatively independent of the horizontal coordinate, x. Deconvolution using the light sheet model yields comparable resolution throughout the FOV.

Fig. 4
Fig. 4

Light-sheet microscopy scan of the musculature of a zebrafish larva. Two distant sections along the light sheet propagation axis (x) are shown at x = +45 μm (a) and x = −10 μm (c). Two-dimensional sections are shown at y = −40 μm (b) and at z = −10 μm (d). The thickness of each section is 7.4 μm. The transverse view of the musculature (b) shows the diameter of individual muscle fibers, which vary in size. In the longitudinal views of the musculature (c, d), a fragment of the V-shaped myosepta separating two muscle blocks can be appreciated: in (c) the myosepta (dark diagonal line) extends from (y = −20 μm, z = 20 μm) to (y = 10 μm, z = 50 μm), and in (d) extends from (x = 70 μm, z = 15 μm) to (x = 40 μm, z = 45 μm).

Equations (2)

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P ( u , v ) = exp ( 2 π i α ( u 3 + v 3 ) ) = exp ( 2 π i α u 3 ) exp ( 2 π i α v 3 ) ,
ϕ ( 4 ) ( ρ , θ , h 0 ) = B h 0 3 ρ cos θ 1 2 ( 2 C cos 2 θ + D ) h 0 2 ρ 2 + E h 0 ρ 3 cos θ 1 4 F ρ 4

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