Abstract

We describe a remote focal scanning technique for optical projection tomography (OPT) implemented with an electrically tunable lens (ETL) that removes the need to scan the specimen or objective lens. Using a 4× objective lens the average spatial resolution is improved by ∼46% and the light collection efficiency by a factor of ∼6.76, thereby enabling increased acquisition speed and reduced light dose. This convenient implementation is particularly appropriate for lower magnifications and larger sample diameters where axial objective scanning would encounter problems with speed and stability.

© 2014 Optical Society of America

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    [Crossref] [PubMed]
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2014 (2)

2013 (2)

2012 (2)

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

2011 (3)

2010 (3)

2008 (5)

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

J.R. Walls, L. Coultas, J. Rossant, and R.M. Henkelman, “Three-dimensional analysis of vascular development in the mouse embryo,” PLoS ONE 3, e2853 (2008).
[Crossref] [PubMed]

C. Dunsby, “Optically sectioned imaging by oblique plane microscopy,” Opt. Express 16, 20306–20316 (2008).
[Crossref] [PubMed]

2007 (1)

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

2006 (1)

N. Krstajic and S. J. Doran, “Focusing optics of a parallel beam CCD optical tomography apparatus for 3D radiation gel dosimetry,” Phys. Med. Biol. 51, 2055–2075 (2006).
[Crossref] [PubMed]

2005 (2)

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

M. Fauver, E. J. Seibel, J. R. Rahn, M. G. Meyer, F. W. Patten, T. Neumann, and A. C. Nelson, “Three-dimensional imaging of single isolated cell nuclei using optical projection tomography,” Opt. Express 13, 4210–4223 (2005).
[Crossref] [PubMed]

2004 (2)

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

2002 (1)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Ahlgren, U.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Alanentalo, T.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

Altaf, M.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

Andrews, N.

Bain, A.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Baldock, R.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Baldock, R.A.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Bassi, A.

A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt. 16, 100502 (2011).
[Crossref] [PubMed]

Becker, K.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Bicker, G.

Birk, U. J.

Buckland, R.A.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Bugeon, L.

Chen, L.

Cheng, S.

Cheng, Y.-S. L.

Clelland, A.K.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Coultas, L.

J.R. Walls, L. Coultas, J. Rossant, and R.M. Henkelman, “Three-dimensional analysis of vascular development in the mouse embryo,” PLoS ONE 3, e2853 (2008).
[Crossref] [PubMed]

Cuenca, R.

D’Andrea, C.

A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt. 16, 100502 (2011).
[Crossref] [PubMed]

da Silva Xavier, G.

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

Dallman, M. J.

Darrell, A.

Davidson, D.

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Davidson, D.R.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Dazai, J.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

Deininger, K.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Del Bene, F.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Deussing, J. M.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Dodt, H. U.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Doran, S. J.

N. Krstajic and S. J. Doran, “Focusing optics of a parallel beam CCD optical tomography apparatus for 3D radiation gel dosimetry,” Phys. Med. Biol. 51, 2055–2075 (2006).
[Crossref] [PubMed]

Downie, H.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Dunsby, C.

Eder, M.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Fahrbach, F. O.

Fauver, M.

Feng, G.

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Ferran, J.L.

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Fieramonti, L.

A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt. 16, 100502 (2011).
[Crossref] [PubMed]

Fisher, M.E.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Ford, T. N.

Frankel, P.

French, P. M. W.

French, P.M.W.

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

Giese, J. D.

Gleave, J.A.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

Hayenga, J.

Hecksher-Sorensen, J.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Heidrich, M.

Heisterkamp, A.

Helmchen, F.

Henkelman, R.M.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

J.R. Walls, L. Coultas, J. Rossant, and R.M. Henkelman, “Three-dimensional analysis of vascular development in the mouse embryo,” PLoS ONE 3, e2853 (2008).
[Crossref] [PubMed]

Hill, B.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Holmberg, D.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

Hrnblad, A.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

Huisken, J.

F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, and J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Express 21, 21010–21026 (2013).
[Crossref] [PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Jabbour, J. M.

Jahrling, N.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Jo, J. A.

Karin Nilsson, A.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

Kerwin, J.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Konstantinides, N.

Krstajic, N.

N. Krstajic and S. J. Doran, “Focusing optics of a parallel beam CCD optical tomography apparatus for 3D radiation gel dosimetry,” Phys. Med. Biol. 51, 2055–2075 (2006).
[Crossref] [PubMed]

Kumar, S.

Lamb, J. R.

Larefalk, A.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

Leischner, U.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Lerch, J.P.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

Lindsay, S.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Lorbeer, C.

Lorbeer, R.

Loren, C.E.

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

Luis Ferran, J.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

Maitland, K. C.

Malik, B. H.

Martinez-de-la-Torre, M.

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Martnez-de-la-Torre, M.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

Mauch, C. P.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Mayans, S.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

McGinty, J.

Mertz, J.

Meyer, H.

Meyer, M. G.

Miao, Q.

Mione, M.

A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt. 16, 100502 (2011).
[Crossref] [PubMed]

Mondragon, A.

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

Murphy, P.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Nelson, A. C.

Neumann, T.

Nieman, B.J.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

Ntziachristos, V.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

Ojeda, D. F. R.

Olsovsky, C.

Patten, F. W.

Perrimon, N.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

Perry, P.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Pitsouli, C.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

Puelles, L.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Rahn, J. R.

Razansky, D.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

Rieckher, M.

Ripoll, J.

Robson, S.C.

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Ross, A.

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Rossant, J.

J.R. Walls, L. Coultas, J. Rossant, and R.M. Henkelman, “Three-dimensional analysis of vascular development in the mouse embryo,” PLoS ONE 3, e2853 (2008).
[Crossref] [PubMed]

Rutter, G.A.

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

Sarasa-Renedo, A.

Sarma, S.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

Schierloh, A.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Schmid, B.

Scott, M.

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Seibel, E. J.

Sharpe, J.

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

Stelzer, E. H. K.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Strachan, T.

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Sun, G.

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

Swoger, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Tavernarakis, N.

Taylor, H. B.

Tickle, C.

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Valentini, G.

A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt. 16, 100502 (2011).
[Crossref] [PubMed]

Vinegoni, C.

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

Voigt, F. F.

Walls, J.R.

J.R. Walls, L. Coultas, J. Rossant, and R.M. Henkelman, “Three-dimensional analysis of vascular development in the mouse embryo,” PLoS ONE 3, e2853 (2008).
[Crossref] [PubMed]

Wittbrodt, J.

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Wong, M.D.

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

Wright, J. M.

Zieglgansberger, W.

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Biomed. Opt. Express (3)

Bmc. Neurosci. (1)

J. Kerwin, M. Scott, J. Sharpe, L. Puelles, S.C. Robson, M. Martinez-de-la-Torre, J.L. Ferran, G. Feng, R. Baldock, T. Strachan, D. Davidson, and S. Lindsay, “3 dimensional modelling of early human brain development using optical projection tomography,” Bmc. Neurosci. 5, 27 (2004).
[Crossref] [PubMed]

Dev. Biol. (1)

M.E. Fisher, A.K. Clelland, A. Bain, R.A. Baldock, P. Murphy, H. Downie, C. Tickle, D.R. Davidson, and R.A. Buckland, “Integrating technologies for comparing 3D gene expression domains in the developing chick limb,” Dev. Biol. 317, 13–23 (2008).
[Crossref] [PubMed]

Diabetes (1)

T. Alanentalo, A. Hrnblad, S. Mayans, A. Karin Nilsson, J. Sharpe, A. Larefalk, U. Ahlgren, and D. Holmberg, “Quantification and three-dimensional imaging of the insulitis-induced destruction of beta-cells in murine type 1 diabetes,” Diabetes 59, 1756–1764 (2010).
[Crossref] [PubMed]

Diabetologia (1)

G. da Silva Xavier, A. Mondragon, G. Sun, L. Chen, J. McGinty, P.M.W. French, and G.A. Rutter, “Abnormal glucose tolerance and insulin secretion in pancreas-specific Tcf7l2-null mice,” Diabetologia 55, 2667–2676 (2012).
[Crossref] [PubMed]

J. Biomed. Opt. (2)

A. Bassi, L. Fieramonti, C. D’Andrea, M. Mione, and G. Valentini, “In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography,” J. Biomed. Opt. 16, 100502 (2011).
[Crossref] [PubMed]

T. Alanentalo, C.E. Loren, A. Larefalk, J. Sharpe, D. Holmberg, and U. Ahlgren, “High-resolution three-dimensional imaging of islet-infiltrate interactions based on optical projection tomography assessments of the intact adult mouse pancreas,” J. Biomed. Opt. 13, 054070 (2008).
[Crossref] [PubMed]

Nat. Meth. (1)

H. U. Dodt, U. Leischner, A. Schierloh, N. Jahrling, C. P. Mauch, K. Deininger, J. M. Deussing, M. Eder, W. Zieglgansberger, and K. Becker, “Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain,” Nat. Meth. 4, 331–336 (2007).
[Crossref]

Nat. Methods (1)

C. Vinegoni, C. Pitsouli, D. Razansky, N. Perrimon, and V. Ntziachristos, “In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography,” Nat. Methods,  5, 45–47 (2008).
[Crossref]

Neuroscience (1)

S. Lindsay, S. Sarma, M. Martnez-de-la-Torre, J. Kerwin, M. Scott, J. Luis Ferran, R. Baldock, and L. Puelles, “Anatomical and gene expression mapping of the ventral pallium in a three-dimensional model of developing human brain,” Neuroscience 136, 625–632 (2005).
[Crossref] [PubMed]

Opt. Express (5)

Opt. Lett. (2)

Phys. Med. Biol. (1)

N. Krstajic and S. J. Doran, “Focusing optics of a parallel beam CCD optical tomography apparatus for 3D radiation gel dosimetry,” Phys. Med. Biol. 51, 2055–2075 (2006).
[Crossref] [PubMed]

Physiological Genomics (1)

J.A. Gleave, M.D. Wong, J. Dazai, M. Altaf, R.M. Henkelman, J.P. Lerch, and B.J. Nieman, “Neuroanatomical phenotyping of the mouse brain with three-dimensional autofluorescence imaging,” Physiological Genomics 44, 778–785 (2012).
[Crossref] [PubMed]

PLoS ONE (1)

J.R. Walls, L. Coultas, J. Rossant, and R.M. Henkelman, “Three-dimensional analysis of vascular development in the mouse embryo,” PLoS ONE 3, e2853 (2008).
[Crossref] [PubMed]

Science (2)

J. Sharpe, U. Ahlgren, P. Perry, B. Hill, A. Ross, J. Hecksher-Sorensen, R. Baldock, and D. Davidson, “Optical projection tomography as a tool for 3D microscopy and gene expression studies,” Science 296, 541–545 (2002).
[Crossref] [PubMed]

J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, “Optical sectioning deep inside live embryos by selective plane illumination microscopy,” Science 305, 1007–1009 (2004).
[Crossref] [PubMed]

Supplementary Material (2)

» Media 1: AVI (437 KB)     
» Media 2: AVI (1061 KB)     

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Figures (6)

Fig. 1
Fig. 1 Schematic of RFS-OPT setup. O – 4× objective lens, AP – aperture, TL – 200 mm focal length tube lens, R1 – 100 mm focal length relay lens, OL – −100 mm focal length offset lens, ETL – electrically tunable lens, R2 – 100 mm focal length relay lens, EF –emission filter, ϕ – sample diameter.
Fig. 2
Fig. 2 Reconstructed X–Y and X–Z image slices of a bead acquired with RFS-OPT (a, b) and standard OPT (c, d). (e) Line plots and Gaussian fits through the Y axis of the reconstructed bead in (a,c) for the standard OPT (red line) and RFS-OPT (blue line) systems showing relative intensity data. Scale bar: 10 μm.
Fig. 3
Fig. 3 Reconstructed maximum intensity projection images and video ( Media 1) of dense bead phantom acquired with (a) RFS and (b) standard OPT plotted for comparison with absolute intensity scales; (c) Intensity line profiles from figures (a, b) as dash lines indicated. It is noted that the standard OPT intensity data has been multiplied by 8 for clarity. Scale bar: 50 μm.
Fig. 4
Fig. 4 A section of reconstructed maximum intensity projection images of clusters of cells that exhibit mCherry labelling in the nucleus acquired with (a) RFS-OPT and (b) standard OPT plotted with absolute intensity scales for comparison; Zoom in MIPs of regions of interest of (c) RFS-OPT and (d) Standard OPT (rectangular boxes in (a,b)). It is noted that the standard OPT intensity data has been multiplied by 4 for clarity. Scale bar: (a, b) 200 μm; (c,d) 20 μm.
Fig. 5
Fig. 5 A snapshot of the 3-D reconstruction of a cell suspension that exhibits mCherry expression in the nucleus. Nuclei in red were identified in both standard and RFS-OPT systems, while nuclei in green were only identified using the RFS-OPT acquisition ( Media 2). Scale bar: 200 μm.
Fig. 6
Fig. 6 A section of reconstructed maximum intensity projection images of a live fluorescent Casper:Fli-GFP transgenic zebrafish (at 4 days post-fertilisation) acquired with (a) RFS-OPT and (b) standard OPT plotted with absolute intensity scales for comparison. It is noted that the standard OPT intensity data has been multiplied by 4 for clarity. Scale bar: 200 μm.

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