Abstract

Although temporally focused wide-field two-photon microscopy (TFM) can perform depth resolved wide field imaging, it cannot avoid the image degradation due to scattering of excitation and emission photons when imaging in a turbid medium. Further, its axial resolution is inferior to standard point-scanning two-photon microscopy. We implemented a structured light illumination for TFM and have shown that it can effectively reject the out-of-focus scattered emission photons improving image contrast. Further, the depth resolution of the improved system is dictated by the spatial frequency of the structure light with the potential of attaining depth resolution better than point-scanning two-photon microscopy.

© 2013 OSA

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References

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2013 (2)

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

H. Dana, N. Kruger, A. Ellman, and S. Shoham, “Line temporal focusing characteristics in transparent and scattering media,” Opt. Express 21(5), 5677–5687 (2013).
[Crossref] [PubMed]

2012 (7)

L. C. Cheng, C. Y. Chang, C. Y. Lin, K. C. Cho, W. C. Yen, N. S. Chang, C. Xu, C. Y. Dong, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

J. Michaelson, H. J. Choi, P. So, and H. D. Huang, “Depth-resolved cellular microrheology using HiLo microscopy,” Biomed. Opt. Express 3(6), 1241–1255 (2012).
[Crossref] [PubMed]

Y. C. Li, L. C. Cheng, C. Y. Chang, C. H. Lien, P. J. Campagnola, and S. J. Chen, “Fast multiphoton microfabrication of freeform polymer microstructures by spatiotemporal focusing and patterned excitation,” Opt. Express 20(17), 19030–19038 (2012).
[Crossref] [PubMed]

H. Choi, D. S. Tzeranis, J. W. Cha, P. Clémenceau, S. J. de Jong, L. K. van Geest, J. H. Moon, I. V. Yannas, and P. T. So, “3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation,” Opt. Express 20(24), 26219–26235 (2012).
[Crossref] [PubMed]

D. Bhattacharya, V. R. Singh, C. Zhi, P. T. C. So, P. Matsudaira, and G. Barbastathis, “Three dimensional HiLo-based structured illumination for a digital scanned laser sheet microscopy (DSLM) in thick tissue imaging,” Opt. Express 20(25), 27337–27347 (2012).
[Crossref] [PubMed]

L. C. Cheng, C. Y. Chang, W. C. Yen, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning of thick tissues,” Proc. SPIE 8520, 85200N, 85200N-8 (2012).
[Crossref]

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt. 17(2), 021105 (2012).
[Crossref] [PubMed]

2011 (4)

D. Lim, T. N. Ford, K. K. Chu, and J. Mertz, “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy,” J. Biomed. Opt. 16(1), 016014 (2011).
[Crossref] [PubMed]

E. Y. S. Yew, H. J. Choi, D. Kim, and P. T. C. So, “Wide-field two-photon microscopy with temporal focusing and HiLo background rejection,” Proc. SPIE 7903, 79031O, 79031O-6 (2011).
[Crossref]

O. D. Therrien, B. Aubé, S. Pagès, P. D. Koninck, and D. Côté, “Wide-field multiphoton imaging of cellular dynamics in thick tissue by temporal focusing and patterned illumination,” Biomed. Opt. Express 2(3), 696–704 (2011).
[Crossref] [PubMed]

H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express 19(6), 4937–4948 (2011).
[Crossref] [PubMed]

2010 (6)

D. Kim and P. T. C. So, “High-throughput three-dimensional lithographic microfabrication,” Opt. Lett. 35(10), 1602–1604 (2010).
[Crossref] [PubMed]

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

B. K. Andrasfalvy, B. V. Zemelman, J. Y. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A. 107(26), 11981–11986 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

P. Sun and Y. Wang, “Measurements of optical parameters of phantom solution and bulk animal tissues in vitro at 650 nm,” Opt. Laser Technol. 42(1), 1–7 (2010).
[Crossref]

2009 (2)

A. Weigel, D. Schild, and A. Zeug, “Resolution in the ApoTome and the confocal laser scanning microscope: comparison,” J. Biomed. Opt. 14(1), 014022 (2009).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express 17(7), 5391–5401 (2009).
[Crossref] [PubMed]

2008 (5)

D. Lim, K. K. Chu, and J. Mertz, “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy,” Opt. Lett. 33(16), 1819–1821 (2008).
[Crossref] [PubMed]

A. Vaziri, J. Y. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221–20226 (2008).
[Crossref] [PubMed]

N. Ji, J. C. Magee, and E. Betzig, “High-speed, low-photodamage nonlinear imaging using passive pulse splitters,” Nat. Methods 5(2), 197–202 (2008).
[Crossref] [PubMed]

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

D. Karadaglić and T. Wilson, “Image formation in structured illumination wide-field fluorescence microscopy,” Micron 39(7), 808–818 (2008).
[Crossref] [PubMed]

2007 (1)

2006 (1)

2005 (2)

2003 (1)

C. Y. Dong, K. Koenig, and P. So, “Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium,” J. Biomed. Opt. 8(3), 450–459 (2003).
[Crossref] [PubMed]

1997 (1)

1991 (1)

1990 (1)

C. J. R. Sheppard and M. Gu, “Image formation in two-photon fluorescence microscopy,” Optik (Stuttg.) 86, 104–106 (1990).

1969 (1)

Agard, D. A.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Andrasfalvy, B. K.

B. K. Andrasfalvy, B. V. Zemelman, J. Y. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A. 107(26), 11981–11986 (2010).
[Crossref] [PubMed]

Anselmi, F.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Aubé, B.

Bahlmann, K.

Barbastathis, G.

Begue, A.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

Bègue, A.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Betzig, E.

N. Ji, J. C. Magee, and E. Betzig, “High-speed, low-photodamage nonlinear imaging using passive pulse splitters,” Nat. Methods 5(2), 197–202 (2008).
[Crossref] [PubMed]

Bhattacharya, D.

Bradley, J.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

Buehler, C.

Campagnola, P. J.

Cande, W. Z.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Carlton, P. M.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Cha, J. W.

Chang, C. Y.

Chang, N. S.

Chen, S. J.

Cheng, L. C.

Cho, K. C.

Choi, H.

Choi, H. J.

J. Michaelson, H. J. Choi, P. So, and H. D. Huang, “Depth-resolved cellular microrheology using HiLo microscopy,” Biomed. Opt. Express 3(6), 1241–1255 (2012).
[Crossref] [PubMed]

E. Y. S. Yew, H. J. Choi, D. Kim, and P. T. C. So, “Wide-field two-photon microscopy with temporal focusing and HiLo background rejection,” Proc. SPIE 7903, 79031O, 79031O-6 (2011).
[Crossref]

Chu, K. K.

D. Lim, T. N. Ford, K. K. Chu, and J. Mertz, “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy,” J. Biomed. Opt. 16(1), 016014 (2011).
[Crossref] [PubMed]

D. Lim, K. K. Chu, and J. Mertz, “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy,” Opt. Lett. 33(16), 1819–1821 (2008).
[Crossref] [PubMed]

Clémenceau, P.

Côté, D.

Dana, H.

de Jong, S. J.

de Sars, V.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express 17(7), 5391–5401 (2009).
[Crossref] [PubMed]

Dong, C. Y.

Durst, M.

Durst, M. E.

Ellman, A.

Emiliani, V.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express 17(7), 5391–5401 (2009).
[Crossref] [PubMed]

Fantini, S.

Ford, T. N.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt. 17(2), 021105 (2012).
[Crossref] [PubMed]

D. Lim, T. N. Ford, K. K. Chu, and J. Mertz, “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy,” J. Biomed. Opt. 16(1), 016014 (2011).
[Crossref] [PubMed]

Glückstad, J.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Golubovskaya, I. N.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Gu, M.

C. J. R. Sheppard and M. Gu, “Image formation in two-photon fluorescence microscopy,” Optik (Stuttg.) 86, 104–106 (1990).

Gustafsson, M. G. L.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Heffer, E. L.

Huang, H. D.

Isacoff, E. Y.

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Ji, N.

N. Ji, J. C. Magee, and E. Betzig, “High-speed, low-photodamage nonlinear imaging using passive pulse splitters,” Nat. Methods 5(2), 197–202 (2008).
[Crossref] [PubMed]

Juskaitis, R.

Karadaglic, D.

D. Karadaglić and T. Wilson, “Image formation in structured illumination wide-field fluorescence microscopy,” Micron 39(7), 808–818 (2008).
[Crossref] [PubMed]

Kim, D.

E. Y. S. Yew, H. J. Choi, D. Kim, and P. T. C. So, “Wide-field two-photon microscopy with temporal focusing and HiLo background rejection,” Proc. SPIE 7903, 79031O, 79031O-6 (2011).
[Crossref]

D. Kim and P. T. C. So, “High-throughput three-dimensional lithographic microfabrication,” Opt. Lett. 35(10), 1602–1604 (2010).
[Crossref] [PubMed]

Kim, J.

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

Kim, K. H.

Koenig, K.

C. Y. Dong, K. Koenig, and P. So, “Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium,” J. Biomed. Opt. 8(3), 450–459 (2003).
[Crossref] [PubMed]

Koninck, P. D.

Kruger, N.

Lee, W. C. A.

Leshem, B.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

Li, Y. C.

Lien, C. H.

Lim, D.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt. 17(2), 021105 (2012).
[Crossref] [PubMed]

D. Lim, T. N. Ford, K. K. Chu, and J. Mertz, “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy,” J. Biomed. Opt. 16(1), 016014 (2011).
[Crossref] [PubMed]

D. Lim, K. K. Chu, and J. Mertz, “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy,” Opt. Lett. 33(16), 1819–1821 (2008).
[Crossref] [PubMed]

Lin, C. Y.

Magee, J. C.

N. Ji, J. C. Magee, and E. Betzig, “High-speed, low-photodamage nonlinear imaging using passive pulse splitters,” Nat. Methods 5(2), 197–202 (2008).
[Crossref] [PubMed]

Matsudaira, P.

Mertz, J.

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt. 17(2), 021105 (2012).
[Crossref] [PubMed]

D. Lim, T. N. Ford, K. K. Chu, and J. Mertz, “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy,” J. Biomed. Opt. 16(1), 016014 (2011).
[Crossref] [PubMed]

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

D. Lim, K. K. Chu, and J. Mertz, “Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy,” Opt. Lett. 33(16), 1819–1821 (2008).
[Crossref] [PubMed]

Michaelson, J.

Moes, C. J.

Moon, J. H.

Nedivi, E.

Neil, M. A. A.

Oron, D.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express 17(7), 5391–5401 (2009).
[Crossref] [PubMed]

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

Pagès, S.

Papagiakoumou, E.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express 17(7), 5391–5401 (2009).
[Crossref] [PubMed]

Prahl, S. A.

Ragan, T.

Schild, D.

A. Weigel, D. Schild, and A. Zeug, “Resolution in the ApoTome and the confocal laser scanning microscope: comparison,” J. Biomed. Opt. 14(1), 014022 (2009).
[Crossref] [PubMed]

Schwartz, O.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

Sedat, J. W.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Shank, C. V.

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

A. Vaziri, J. Y. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221–20226 (2008).
[Crossref] [PubMed]

Shao, L.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Sheppard, C. J. R.

C. J. R. Sheppard and M. Gu, “Image formation in two-photon fluorescence microscopy,” Optik (Stuttg.) 86, 104–106 (1990).

Shoham, S.

Shroff, H.

A. Vaziri, J. Y. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221–20226 (2008).
[Crossref] [PubMed]

Silberberg, Y.

Singh, V. R.

So, P.

J. Michaelson, H. J. Choi, P. So, and H. D. Huang, “Depth-resolved cellular microrheology using HiLo microscopy,” Biomed. Opt. Express 3(6), 1241–1255 (2012).
[Crossref] [PubMed]

C. Y. Dong, K. Koenig, and P. So, “Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium,” J. Biomed. Opt. 8(3), 450–459 (2003).
[Crossref] [PubMed]

So, P. T.

So, P. T. C.

Stell, B. M.

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

Stokseth, P. A.

Sun, P.

P. Sun and Y. Wang, “Measurements of optical parameters of phantom solution and bulk animal tissues in vitro at 650 nm,” Opt. Laser Technol. 42(1), 1–7 (2010).
[Crossref]

Tal, E.

Tang, J. Y.

B. K. Andrasfalvy, B. V. Zemelman, J. Y. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A. 107(26), 11981–11986 (2010).
[Crossref] [PubMed]

A. Vaziri, J. Y. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221–20226 (2008).
[Crossref] [PubMed]

Therrien, O. D.

Tzeranis, D. S.

van Geest, L. K.

van Gemert, M. J.

van Howe, J.

van Marie, J.

van Staveren, H. J.

Vaziri, A.

B. K. Andrasfalvy, B. V. Zemelman, J. Y. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A. 107(26), 11981–11986 (2010).
[Crossref] [PubMed]

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

A. Vaziri, J. Y. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221–20226 (2008).
[Crossref] [PubMed]

Wang, C. J. R.

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

Wang, Y.

P. Sun and Y. Wang, “Measurements of optical parameters of phantom solution and bulk animal tissues in vitro at 650 nm,” Opt. Laser Technol. 42(1), 1–7 (2010).
[Crossref]

Weigel, A.

A. Weigel, D. Schild, and A. Zeug, “Resolution in the ApoTome and the confocal laser scanning microscope: comparison,” J. Biomed. Opt. 14(1), 014022 (2009).
[Crossref] [PubMed]

Wilson, T.

D. Karadaglić and T. Wilson, “Image formation in structured illumination wide-field fluorescence microscopy,” Micron 39(7), 808–818 (2008).
[Crossref] [PubMed]

M. A. A. Neil, R. Juskaitis, and T. Wilson, “Method of obtaining optical sectioning by using structured light in a conventional microscope,” Opt. Lett. 22(24), 1905–1907 (1997).
[Crossref] [PubMed]

Xu, C.

Yannas, I. V.

Yen, W. C.

L. C. Cheng, C. Y. Chang, C. Y. Lin, K. C. Cho, W. C. Yen, N. S. Chang, C. Xu, C. Y. Dong, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

L. C. Cheng, C. Y. Chang, W. C. Yen, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning of thick tissues,” Proc. SPIE 8520, 85200N, 85200N-8 (2012).
[Crossref]

Yew, E. Y. S.

E. Y. S. Yew, H. J. Choi, D. Kim, and P. T. C. So, “Wide-field two-photon microscopy with temporal focusing and HiLo background rejection,” Proc. SPIE 7903, 79031O, 79031O-6 (2011).
[Crossref]

Zemelman, B. V.

B. K. Andrasfalvy, B. V. Zemelman, J. Y. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A. 107(26), 11981–11986 (2010).
[Crossref] [PubMed]

Zeug, A.

A. Weigel, D. Schild, and A. Zeug, “Resolution in the ApoTome and the confocal laser scanning microscope: comparison,” J. Biomed. Opt. 14(1), 014022 (2009).
[Crossref] [PubMed]

Zhi, C.

Zhu, G. H.

Zipfel, W.

Appl. Opt. (1)

Biomed. Opt. Express (2)

Biophys. J. (1)

M. G. L. Gustafsson, L. Shao, P. M. Carlton, C. J. R. Wang, I. N. Golubovskaya, W. Z. Cande, D. A. Agard, and J. W. Sedat, “Three-dimensional resolution doubling in wide-field fluorescence microscopy by structured illumination,” Biophys. J. 94(12), 4957–4970 (2008).
[Crossref] [PubMed]

J. Biomed. Opt. (5)

C. Y. Dong, K. Koenig, and P. So, “Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium,” J. Biomed. Opt. 8(3), 450–459 (2003).
[Crossref] [PubMed]

A. Weigel, D. Schild, and A. Zeug, “Resolution in the ApoTome and the confocal laser scanning microscope: comparison,” J. Biomed. Opt. 14(1), 014022 (2009).
[Crossref] [PubMed]

D. Lim, T. N. Ford, K. K. Chu, and J. Mertz, “Optically sectioned in vivo imaging with speckle illumination HiLo microscopy,” J. Biomed. Opt. 16(1), 016014 (2011).
[Crossref] [PubMed]

J. Mertz and J. Kim, “Scanning light-sheet microscopy in the whole mouse brain with HiLo background rejection,” J. Biomed. Opt. 15(1), 016027 (2010).
[Crossref] [PubMed]

T. N. Ford, D. Lim, and J. Mertz, “Fast optically sectioned fluorescence HiLo endomicroscopy,” J. Biomed. Opt. 17(2), 021105 (2012).
[Crossref] [PubMed]

J. Opt. Soc. Am. (1)

Micron (1)

D. Karadaglić and T. Wilson, “Image formation in structured illumination wide-field fluorescence microscopy,” Micron 39(7), 808–818 (2008).
[Crossref] [PubMed]

Nat. Methods (2)

N. Ji, J. C. Magee, and E. Betzig, “High-speed, low-photodamage nonlinear imaging using passive pulse splitters,” Nat. Methods 5(2), 197–202 (2008).
[Crossref] [PubMed]

E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, “Scanless two-photon excitation of channelrhodopsin-2,” Nat. Methods 7(10), 848–854 (2010).
[Crossref] [PubMed]

Nat. Photonics (1)

E. Papagiakoumou, A. Begue, B. Leshem, O. Schwartz, B. M. Stell, J. Bradley, D. Oron, and V. Emiliani, “Functional patterned multiphoton excitation deep inside scattering tissue,” Nat. Photonics 7(4), 274–278 (2013).
[Crossref]

Opt. Express (12)

D. Oron, E. Tal, and Y. Silberberg, “Scanningless depth-resolved microscopy,” Opt. Express 13(5), 1468–1476 (2005).
[Crossref] [PubMed]

G. H. Zhu, J. van Howe, M. Durst, W. Zipfel, and C. Xu, “Simultaneous spatial and temporal focusing of femtosecond pulses,” Opt. Express 13(6), 2153–2159 (2005).
[Crossref] [PubMed]

M. E. Durst, G. H. Zhu, and C. Xu, “Simultaneous spatial and temporal focusing for axial scanning,” Opt. Express 14(25), 12243–12254 (2006).
[Crossref] [PubMed]

K. H. Kim, C. Buehler, K. Bahlmann, T. Ragan, W. C. A. Lee, E. Nedivi, E. L. Heffer, S. Fantini, and P. T. C. So, “Multifocal multiphoton microscopy based on multianode photomultiplier tubes,” Opt. Express 15(18), 11658–11678 (2007).
[Crossref] [PubMed]

A. Vaziri and C. V. Shank, “Ultrafast widefield optical sectioning microscopy by multifocal temporal focusing,” Opt. Express 18(19), 19645–19655 (2010).
[Crossref] [PubMed]

E. Papagiakoumou, V. de Sars, V. Emiliani, and D. Oron, “Temporal focusing with spatially modulated excitation,” Opt. Express 17(7), 5391–5401 (2009).
[Crossref] [PubMed]

H. Dana and S. Shoham, “Numerical evaluation of temporal focusing characteristics in transparent and scattering media,” Opt. Express 19(6), 4937–4948 (2011).
[Crossref] [PubMed]

L. C. Cheng, C. Y. Chang, C. Y. Lin, K. C. Cho, W. C. Yen, N. S. Chang, C. Xu, C. Y. Dong, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning,” Opt. Express 20(8), 8939–8948 (2012).
[Crossref] [PubMed]

Y. C. Li, L. C. Cheng, C. Y. Chang, C. H. Lien, P. J. Campagnola, and S. J. Chen, “Fast multiphoton microfabrication of freeform polymer microstructures by spatiotemporal focusing and patterned excitation,” Opt. Express 20(17), 19030–19038 (2012).
[Crossref] [PubMed]

H. Choi, D. S. Tzeranis, J. W. Cha, P. Clémenceau, S. J. de Jong, L. K. van Geest, J. H. Moon, I. V. Yannas, and P. T. So, “3D-resolved fluorescence and phosphorescence lifetime imaging using temporal focusing wide-field two-photon excitation,” Opt. Express 20(24), 26219–26235 (2012).
[Crossref] [PubMed]

D. Bhattacharya, V. R. Singh, C. Zhi, P. T. C. So, P. Matsudaira, and G. Barbastathis, “Three dimensional HiLo-based structured illumination for a digital scanned laser sheet microscopy (DSLM) in thick tissue imaging,” Opt. Express 20(25), 27337–27347 (2012).
[Crossref] [PubMed]

H. Dana, N. Kruger, A. Ellman, and S. Shoham, “Line temporal focusing characteristics in transparent and scattering media,” Opt. Express 21(5), 5677–5687 (2013).
[Crossref] [PubMed]

Opt. Laser Technol. (1)

P. Sun and Y. Wang, “Measurements of optical parameters of phantom solution and bulk animal tissues in vitro at 650 nm,” Opt. Laser Technol. 42(1), 1–7 (2010).
[Crossref]

Opt. Lett. (3)

Optik (Stuttg.) (1)

C. J. R. Sheppard and M. Gu, “Image formation in two-photon fluorescence microscopy,” Optik (Stuttg.) 86, 104–106 (1990).

Proc. Natl. Acad. Sci. U.S.A. (2)

A. Vaziri, J. Y. Tang, H. Shroff, and C. V. Shank, “Multilayer three-dimensional super resolution imaging of thick biological samples,” Proc. Natl. Acad. Sci. U.S.A. 105(51), 20221–20226 (2008).
[Crossref] [PubMed]

B. K. Andrasfalvy, B. V. Zemelman, J. Y. Tang, and A. Vaziri, “Two-photon single-cell optogenetic control of neuronal activity by sculpted light,” Proc. Natl. Acad. Sci. U.S.A. 107(26), 11981–11986 (2010).
[Crossref] [PubMed]

Proc. SPIE (2)

E. Y. S. Yew, H. J. Choi, D. Kim, and P. T. C. So, “Wide-field two-photon microscopy with temporal focusing and HiLo background rejection,” Proc. SPIE 7903, 79031O, 79031O-6 (2011).
[Crossref]

L. C. Cheng, C. Y. Chang, W. C. Yen, and S. J. Chen, “Spatiotemporal focusing-based widefield multiphoton microscopy for fast optical sectioning of thick tissues,” Proc. SPIE 8520, 85200N, 85200N-8 (2012).
[Crossref]

Other (1)

D. A. Boas, C. Pitris, and N. Ramanujam, eds., Handbook of Biomedical Optics (CRC Press, 2011), Chap. 5.

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