Abstract

There is a need for a noninvasive technique to monitor living pluripotent stem cell condition without any labeling. We present an optical imaging technique that is able to capture information about optical path difference through the cell and cell adhesion properties simultaneously using a combination of quantitative phase microscopy (QPM) and interference reflection microscopy (IRM) techniques. As a novel application of QPM and IRM, this multimodal imaging technique demonstrated its ability to distinguish the undifferentiated status of human induced pluripotent stem (hiPS) cells quantitatively based on the variation of optical path difference between the nucleus and cytoplasm as well as hiPS cell-specific cell adhesion properties.

© 2012 OSA

Full Article  |  PDF Article

References

  • View by:
  • |
  • |
  • |

  1. G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett.29(21), 2503–2505 (2004).
    [CrossRef] [PubMed]
  2. T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, “Hilbert phase microscopy for investigating fast dynamics in transparent systems,” Opt. Lett.30(10), 1165–1167 (2005).
    [CrossRef] [PubMed]
  3. X. Li, T. Yamauchi, H. Iwai, Y. Yamashita, H. Zhang, and T. Hiruma, “Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples,” Opt. Lett.31(12), 1830–1832 (2006).
    [CrossRef] [PubMed]
  4. G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
    [CrossRef] [PubMed]
  5. Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
    [CrossRef] [PubMed]
  6. M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
    [CrossRef] [PubMed]
  7. Z. Wang, L. J. Millet, M. Mir, H. Ding, S. Unarunotai, J. A. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011).
    [CrossRef] [PubMed]
  8. T. Yamauchi, H. Iwai, M. Miwa, and Y. Yamashita, “Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology,” Opt. Express16(16), 12227–12238 (2008).
    [CrossRef] [PubMed]
  9. G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006).
    [CrossRef] [PubMed]
  10. H. Verschueren, “Interference reflection microscopy in cell biology: methodology and applications,” J. Cell Sci.75, 279–301 (1985).
    [PubMed]
  11. J. Bereiter-Hahn, C. H. Fox, and B. Thorell, “Quantitative reflection contrast microscopy of living cells,” J. Cell Biol.82(3), 767–779 (1979).
    [CrossRef] [PubMed]
  12. R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
    [CrossRef] [PubMed]
  13. M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
    [CrossRef] [PubMed]
  14. K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
    [CrossRef] [PubMed]
  15. J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
    [PubMed]
  16. A. Llobet, M. Wu, and L. Lagnado, “The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin,” J. Cell Biol.182(5), 1017–1028 (2008).
    [CrossRef] [PubMed]
  17. G. Itoh and S. Yumura, “A novel mitosis-specific dynamic actin structure in Dictyostelium cells,” J. Cell Sci.120(24), 4302–4309 (2007).
    [CrossRef] [PubMed]
  18. C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
    [CrossRef] [PubMed]
  19. L. Limozin and K. Sengupta, “Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion,” ChemPhysChem10(16), 2752–2768 (2009).
    [CrossRef] [PubMed]
  20. I. Weber, “Reflection interference contrast microscopy,” Methods Enzymol.361, 34–47 (2003).
    [CrossRef] [PubMed]
  21. T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
    [CrossRef] [PubMed]
  22. W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
    [CrossRef] [PubMed]
  23. W. J. Choi, I. Jeon, S. G. Ahn, J. H. Yoon, S. Kim, and B. H. Lee, “Full-field optical coherence microscopy for identifying live cancer cells by quantitative measurement of refractive index distribution,” Opt. Express18(22), 23285–23295 (2010).
    [CrossRef] [PubMed]

2011 (2)

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Z. Wang, L. J. Millet, M. Mir, H. Ding, S. Unarunotai, J. A. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011).
[CrossRef] [PubMed]

2010 (1)

2009 (1)

L. Limozin and K. Sengupta, “Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion,” ChemPhysChem10(16), 2752–2768 (2009).
[CrossRef] [PubMed]

2008 (5)

A. Llobet, M. Wu, and L. Lagnado, “The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin,” J. Cell Biol.182(5), 1017–1028 (2008).
[CrossRef] [PubMed]

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

T. Yamauchi, H. Iwai, M. Miwa, and Y. Yamashita, “Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology,” Opt. Express16(16), 12227–12238 (2008).
[CrossRef] [PubMed]

C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
[CrossRef] [PubMed]

2007 (2)

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

G. Itoh and S. Yumura, “A novel mitosis-specific dynamic actin structure in Dictyostelium cells,” J. Cell Sci.120(24), 4302–4309 (2007).
[CrossRef] [PubMed]

2006 (4)

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006).
[CrossRef] [PubMed]

X. Li, T. Yamauchi, H. Iwai, Y. Yamashita, H. Zhang, and T. Hiruma, “Full-field quantitative phase imaging by white-light interferometry with active phase stabilization and its application to biological samples,” Opt. Lett.31(12), 1830–1832 (2006).
[CrossRef] [PubMed]

2005 (2)

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
[CrossRef] [PubMed]

T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, “Hilbert phase microscopy for investigating fast dynamics in transparent systems,” Opt. Lett.30(10), 1165–1167 (2005).
[CrossRef] [PubMed]

2004 (1)

2003 (1)

I. Weber, “Reflection interference contrast microscopy,” Methods Enzymol.361, 34–47 (2003).
[CrossRef] [PubMed]

1992 (1)

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

1985 (1)

H. Verschueren, “Interference reflection microscopy in cell biology: methodology and applications,” J. Cell Sci.75, 279–301 (1985).
[PubMed]

1982 (1)

J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
[PubMed]

1979 (1)

J. Bereiter-Hahn, C. H. Fox, and B. Thorell, “Quantitative reflection contrast microscopy of living cells,” J. Cell Biol.82(3), 767–779 (1979).
[CrossRef] [PubMed]

Adamson, E. A.

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Ahn, S. G.

Akatsu, T.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Aranda-Espinoza, H.

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

Badizadegan, K.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
[CrossRef] [PubMed]

G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett.29(21), 2503–2505 (2004).
[CrossRef] [PubMed]

Barsukov, I. L.

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Bashir, R.

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Bednarz, M.

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Bereiter-Hahn, J.

J. Bereiter-Hahn, C. H. Fox, and B. Thorell, “Quantitative reflection contrast microscopy of living cells,” J. Cell Biol.82(3), 767–779 (1979).
[CrossRef] [PubMed]

Best, C. A.

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
[CrossRef] [PubMed]

Bobkov, A.

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Calle, Y.

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

Cassiman, J. J.

J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
[PubMed]

Choi, C. K.

C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
[CrossRef] [PubMed]

Choi, W.

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

Choi, W. J.

Critchley, D. R.

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Dasari, R. R.

Deflores, L. P.

Diez-Silva, M.

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

Ding, H.

Dunn, G. A.

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

English, A. E.

C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
[CrossRef] [PubMed]

Fang-Yen, C.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

Feld, M. S.

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
[CrossRef] [PubMed]

T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, “Hilbert phase microscopy for investigating fast dynamics in transparent systems,” Opt. Lett.30(10), 1165–1167 (2005).
[CrossRef] [PubMed]

G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett.29(21), 2503–2505 (2004).
[CrossRef] [PubMed]

Fox, C. H.

J. Bereiter-Hahn, C. H. Fox, and B. Thorell, “Quantitative reflection contrast microscopy of living cells,” J. Cell Biol.82(3), 767–779 (1979).
[CrossRef] [PubMed]

Gillette, M. U.

Golding, I.

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Hammer, D.

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

Hiruma, T.

Holt, M. R.

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Ikeda, T.

Itoh, G.

G. Itoh and S. Yumura, “A novel mitosis-specific dynamic actin structure in Dictyostelium cells,” J. Cell Sci.120(24), 4302–4309 (2007).
[CrossRef] [PubMed]

Iwai, H.

Janmey, P.

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

Jennings, L.

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Jeon, I.

Jones, G. E.

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

Kihm, K. D.

C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
[CrossRef] [PubMed]

Kim, S.

Lagnado, L.

A. Llobet, M. Wu, and L. Lagnado, “The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin,” J. Cell Biol.182(5), 1017–1028 (2008).
[CrossRef] [PubMed]

Lee, B. H.

Li, X.

Liddington, R. C.

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Limozin, L.

L. Limozin and K. Sengupta, “Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion,” ChemPhysChem10(16), 2752–2768 (2009).
[CrossRef] [PubMed]

Llobet, A.

A. Llobet, M. Wu, and L. Lagnado, “The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin,” J. Cell Biol.182(5), 1017–1028 (2008).
[CrossRef] [PubMed]

Lue, N.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

Lykotrafitis, G.

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

Margraves, C. H.

C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
[CrossRef] [PubMed]

Millet, L. J.

Mir, M.

Z. Wang, L. J. Millet, M. Mir, H. Ding, S. Unarunotai, J. A. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011).
[CrossRef] [PubMed]

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Miwa, M.

Nagata, N.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Oh, S.

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

Park, Y. K.

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

Popescu, G.

Z. Wang, L. J. Millet, M. Mir, H. Ding, S. Unarunotai, J. A. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011).
[CrossRef] [PubMed]

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, R. R. Dasari, and M. S. Feld, “Diffraction phase microscopy for quantifying cell structure and dynamics,” Opt. Lett.31(6), 775–777 (2006).
[CrossRef] [PubMed]

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
[CrossRef] [PubMed]

T. Ikeda, G. Popescu, R. R. Dasari, and M. S. Feld, “Hilbert phase microscopy for investigating fast dynamics in transparent systems,” Opt. Lett.30(10), 1165–1167 (2005).
[CrossRef] [PubMed]

G. Popescu, L. P. Deflores, J. C. Vaughan, K. Badizadegan, H. Iwai, R. R. Dasari, and M. S. Feld, “Fourier phase microscopy for investigation of biological structures and dynamics,” Opt. Lett.29(21), 2503–2505 (2004).
[CrossRef] [PubMed]

Prasanth, S. G.

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Rogers, J. A.

Sasaki, T.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Saunders, R. M.

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Sengupta, K.

L. Limozin and K. Sengupta, “Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion,” ChemPhysChem10(16), 2752–2768 (2009).
[CrossRef] [PubMed]

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

Shen, Z.

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Smith, L.

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

Suda, T.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Suresh, S.

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

Sutton, D. H.

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

Takahashi, N.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Tamura, T.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Tanaka, S.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Thorell, B.

J. Bereiter-Hahn, C. H. Fox, and B. Thorell, “Quantitative reflection contrast microscopy of living cells,” J. Cell Biol.82(3), 767–779 (1979).
[CrossRef] [PubMed]

Udagawa, N.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Unarunotai, S.

van den Berghe, H.

J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
[PubMed]

van der Schueren, B.

J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
[PubMed]

van Leuven, F.

J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
[PubMed]

Vaughan, J. C.

Verschueren, H.

H. Verschueren, “Interference reflection microscopy in cell biology: methodology and applications,” J. Cell Sci.75, 279–301 (1985).
[PubMed]

Wang, Z.

Z. Wang, L. J. Millet, M. Mir, H. Ding, S. Unarunotai, J. A. Rogers, M. U. Gillette, and G. Popescu, “Spatial light interference microscopy (SLIM),” Opt. Express19(2), 1016–1026 (2011).
[CrossRef] [PubMed]

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Weber, I.

I. Weber, “Reflection interference contrast microscopy,” Methods Enzymol.361, 34–47 (2003).
[CrossRef] [PubMed]

Wu, M.

A. Llobet, M. Wu, and L. Lagnado, “The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin,” J. Cell Biol.182(5), 1017–1028 (2008).
[CrossRef] [PubMed]

Yamaguchi, A.

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

Yamashita, Y.

Yamauchi, T.

Yoon, J. H.

Yumura, S.

G. Itoh and S. Yumura, “A novel mitosis-specific dynamic actin structure in Dictyostelium cells,” J. Cell Sci.120(24), 4302–4309 (2007).
[CrossRef] [PubMed]

Zhang, H.

Biophys. J. (1)

K. Sengupta, H. Aranda-Espinoza, L. Smith, P. Janmey, and D. Hammer, “Spreading of neutrophils: from activation to migration,” Biophys. J.91(12), 4638–4648 (2006).
[CrossRef] [PubMed]

ChemPhysChem (1)

L. Limozin and K. Sengupta, “Quantitative reflection interference contrast microscopy (RICM) in soft matter and cell adhesion,” ChemPhysChem10(16), 2752–2768 (2009).
[CrossRef] [PubMed]

Eur. J. Cell Biol. (1)

R. M. Saunders, M. R. Holt, L. Jennings, D. H. Sutton, I. L. Barsukov, A. Bobkov, R. C. Liddington, E. A. Adamson, G. A. Dunn, and D. R. Critchley, “Role of vinculin in regulating focal adhesion turnover,” Eur. J. Cell Biol.85(6), 487–500 (2006).
[CrossRef] [PubMed]

J. Biomed. Opt. (2)

G. Popescu, T. Ikeda, C. A. Best, K. Badizadegan, R. R. Dasari, and M. S. Feld, “Erythrocyte structure and dynamics quantified by Hilbert phase microscopy,” J. Biomed. Opt.10(6), 060503 (2005).
[CrossRef] [PubMed]

C. K. Choi, C. H. Margraves, A. E. English, and K. D. Kihm, “Multicontrast microscopy technique to dynamically fingerprint live-cell focal contacts during exposure and replacement of a cytotoxic medium,” J. Biomed. Opt.13(5), 054069 (2008).
[CrossRef] [PubMed]

J. Bone Miner. Res. (1)

T. Akatsu, T. Tamura, N. Takahashi, N. Udagawa, S. Tanaka, T. Sasaki, A. Yamaguchi, N. Nagata, and T. Suda, “Preparation and characterization of a mouse osteoclast-like multinucleated cell population,” J. Bone Miner. Res.7(11), 1297–1306 (1992).
[CrossRef] [PubMed]

J. Cell Biol. (2)

J. Bereiter-Hahn, C. H. Fox, and B. Thorell, “Quantitative reflection contrast microscopy of living cells,” J. Cell Biol.82(3), 767–779 (1979).
[CrossRef] [PubMed]

A. Llobet, M. Wu, and L. Lagnado, “The mouth of a dense-core vesicle opens and closes in a concerted action regulated by calcium and amphiphysin,” J. Cell Biol.182(5), 1017–1028 (2008).
[CrossRef] [PubMed]

J. Cell Sci. (3)

G. Itoh and S. Yumura, “A novel mitosis-specific dynamic actin structure in Dictyostelium cells,” J. Cell Sci.120(24), 4302–4309 (2007).
[CrossRef] [PubMed]

J. J. Cassiman, B. van der Schueren, F. van Leuven, and H. van den Berghe, “Qualitative and quantitative differences in spreading of human fibroblasts on various protein coats. Modulation by treatment of the cells with amines,” J. Cell Sci.54, 79–95 (1982).
[PubMed]

H. Verschueren, “Interference reflection microscopy in cell biology: methodology and applications,” J. Cell Sci.75, 279–301 (1985).
[PubMed]

J. Microsc. (1)

M. R. Holt, Y. Calle, D. H. Sutton, D. R. Critchley, G. E. Jones, and G. A. Dunn, “Quantifying cell-matrix adhesion dynamics in living cells using interference reflection microscopy,” J. Microsc.232(1), 73–81 (2008).
[CrossRef] [PubMed]

Methods Enzymol. (1)

I. Weber, “Reflection interference contrast microscopy,” Methods Enzymol.361, 34–47 (2003).
[CrossRef] [PubMed]

Nat. Methods (1)

W. Choi, C. Fang-Yen, K. Badizadegan, S. Oh, N. Lue, R. R. Dasari, and M. S. Feld, “Tomographic phase microscopy,” Nat. Methods4(9), 717–719 (2007).
[CrossRef] [PubMed]

Opt. Express (3)

Opt. Lett. (4)

Proc. Natl. Acad. Sci. U.S.A. (2)

Y. K. Park, M. Diez-Silva, G. Popescu, G. Lykotrafitis, W. Choi, M. S. Feld, and S. Suresh, “Refractive index maps and membrane dynamics of human red blood cells parasitized by Plasmodium falciparum,” Proc. Natl. Acad. Sci. U.S.A.105(37), 13730–13735 (2008).
[CrossRef] [PubMed]

M. Mir, Z. Wang, Z. Shen, M. Bednarz, R. Bashir, I. Golding, S. G. Prasanth, and G. Popescu, “Optical measurement of cycle-dependent cell growth,” Proc. Natl. Acad. Sci. U.S.A.108(32), 13124–13129 (2011).
[CrossRef] [PubMed]

Cited By

OSA participates in CrossRef's Cited-By Linking service. Citing articles from OSA journals and other participating publishers are listed here.

Alert me when this article is cited.


Figures (4)

Fig. 1
Fig. 1

Schematic diagram of QPM-IRM imaging setup. Illumination light of SLD 830nm (red line) for QPM imaging was transmitted through a specimen (S). Transmitted light image was formed by an objective lens (OBJ) and a tube lens (TL2). The image was led to the quantitative phase imaging (QPI) unit. Optical setup of the QPI unit was described in Fig. 2(a) in detail. The QPI unit consisted of a grating apparatus (G), a spatial filter (SF) to be used as a sample and reference image and a couple of relay lenses (L1 and L2). Interference image was formed onto CCD camera (QPM CCD). White LED illumination light (green line) for IRM imaging was selected with a wide-bandwidth band-pass filter (BPF) and irradiated to the bottom side of specimen through an aperture slit (SLT). Reflection interference image form the specimen was formed onto another CCD camera (IRM CCD) by a tube lens (TL1) after being reflected by a long-pass dichroic mirror (DM) through a beam splitter (BS).

Fig. 2
Fig. 2

The principle of QPM-IRM imaging and quantitative measurement results by using grooved glass slides and polystyrene beads whose physical shapes are known. a) Optical diagram of the quantitative phase imaging unit. TL2: Tube lens, G: Phase grating, SF: Spatial filter, L1 and L2: A couple of relay lenses. b) Linearity of QPM measurement by using grooved glass slides; the measured phase difference is plotted as a function of known physical thickness. c) Schematic diagram of IRM. R1: Reflection light from glass-to-medium interface, R2: Reflection light from medium-to-cell interface. Symbols nc, nm and ns indicate the refractive indices of cell, medium and substrate, respectively. d) IRM intensity plotted as a function of bead-to-substrate distance measured by using a polystyrene bead (10 μm in diameter) attached onto a glass-bottom culture dish.

Fig. 3
Fig. 3

QPM-IRM image characteristics of hiPS cells and other types of cells: a) Bright-field (BF) and QPM-IRM images of living cells and 10μm polystyrene bead (PS-bead) were shown. Full grey scale of QPM image indicated OPD of 1 μm. The gray scale of IRM indicated intensity level from 2850 to 3150. The following data were measured from 15 to 20 individual cells (b to d). Scale bar is 10μm. b) The average OPD of nucleus region (white bar) and cytoplasm region (grey bar). Each data was expressed as the mean ± S.D. c) The ratio of cytoplasm-to-nucleus OPD of each cell was expressed as the mean ± S.D. *, P < 0.001. d) The maximal size of close contact spots of each adherent cell was expressed in dot plotted chart (white circles). *, P < 0.001. P means the p-value of the statistical significance test. Refer to subsection 2.4.

Fig. 4
Fig. 4

The quantitative differences between hiPS cells judged to “good” and “no good” status: a) The representative images of “good” (left) and “no good” (right) colonies of hiPS cells. Phase contrast (PhC) microscopy images and QPM-IRM images of white-lined region of PhC. Full gray scale of QPM image indicated OPD of 1 μm. Gray scale of IRM image indicated intensity level from 2850 to 3150. Scale bar is 100 μm. The following data were measured from 619 “good” cells and 433 “no good” cells (b to d). b) The average OPD of nucleus region and of cytoplasm region were expressed as two axis scattergram (“good” in dark blue and “no good” in magenta). c) The ratio of OPD (cytoplasm/nucleus) of each cell was expressed as a box plot. *; P < 0.001. d) Maximal size of close contact adhesion spots of each cell in two groups were expressed as a histogram of number of cells. e) Statistical analysis of maximal size of close contact spots of two groups were shown in a box plot. *, P < 0.001. P means the p-value of statistical significance test. Refer to subsection 2.4.

Metrics