Abstract

We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained through second harmonic generation (SHG) of the 1550-nm pump laser and the 1728-nm and 1900-nm solitons generated through soliton self-frequency shift (SSFS) in a large-mode-area (LMA) fiber. These energetic pulses are well matched to the two-photon excitation peaks of red, cyan and yellow fluorescent proteins (TagRFPs, TagCFPs, and TagYFPs) for efficient excitation. We demonstrate simultaneous 2PM of human melanoma cells expressing a “rainbow” combination of these three fluorescent proteins.

© 2012 OSA

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    [CrossRef] [PubMed]
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2011 (6)

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

K. Hope and M. Bhatia, “Clonal interrogation of stem cells,” Nat. Methods 8(4Suppl), S36–S40 (2011).
[CrossRef] [PubMed]

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

K. Wang and C. Xu, “Wavelength-tunable high-energy soliton pulse generation from a large-mode-area fiber pumped by a time-lens source,” Opt. Lett. 36(6), 942–944 (2011).
[CrossRef] [PubMed]

K. Wang and C. Xu, “Tunable high-energy soliton pulse generation from a large-mode-area fiber and its application to third harmonic generation microscopy,” Appl. Phys. Lett. 99(7), 071112 (2011).
[CrossRef]

2010 (1)

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

2009 (1)

2007 (1)

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

2006 (1)

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

2003 (1)

D. V. Skryabin, F. Luan, J. C. Knight, and P. S. Russell, “Soliton self-frequency shift cancellation in photonic crystal fibers,” Science 301(5640), 1705–1708 (2003).
[CrossRef] [PubMed]

2001 (1)

1999 (2)

N. Nishizawa and T. Goto, “Compact system of wavelength-tunable femtosecond soliton pulse generation using optical fibers,” IEEE Photon. Technol. Lett. 11(3), 325–327 (1999).
[CrossRef]

N. Nishizawa, R. Okamura, and T. Goto, “Simultaneous generation of wavelength tunable two-colored femtosecond soliton pulses using optical fibers,” IEEE Photon. Technol. Lett. 11(4), 421–423 (1999).
[CrossRef]

1994 (1)

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
[CrossRef] [PubMed]

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[CrossRef] [PubMed]

1986 (1)

Bennis, R. A.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Benten, D.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Bhatia, M.

K. Hope and M. Bhatia, “Clonal interrogation of stem cells,” Nat. Methods 8(4Suppl), S36–S40 (2011).
[CrossRef] [PubMed]

Bouvet, M.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Chalfie, M.

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
[CrossRef] [PubMed]

Chandalia, J. K.

Condeelis, J.

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Cornils, K.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Dandri, M.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Denk, W.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[CrossRef] [PubMed]

Draft, R. W.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Drobizhev, M.

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

Durst, M. E.

Eggleton, B. J.

Entenberg, D.

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Euskirchen, G.

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
[CrossRef] [PubMed]

Fan, Z.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Fehse, B.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Gligorijevic, B.

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Gordon, J. P.

Goto, T.

N. Nishizawa, R. Okamura, and T. Goto, “Simultaneous generation of wavelength tunable two-colored femtosecond soliton pulses using optical fibers,” IEEE Photon. Technol. Lett. 11(4), 421–423 (1999).
[CrossRef]

N. Nishizawa and T. Goto, “Compact system of wavelength-tunable femtosecond soliton pulse generation using optical fibers,” IEEE Photon. Technol. Lett. 11(3), 325–327 (1999).
[CrossRef]

Hoffman, R. M.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Hope, K.

K. Hope and M. Bhatia, “Clonal interrogation of stem cells,” Nat. Methods 8(4Suppl), S36–S40 (2011).
[CrossRef] [PubMed]

Hughes, T. E.

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

Jiang, P.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Kang, H.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Kim, P.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Knight, J. C.

D. V. Skryabin, F. Luan, J. C. Knight, and P. S. Russell, “Soliton self-frequency shift cancellation in photonic crystal fibers,” Science 301(5640), 1705–1708 (2003).
[CrossRef] [PubMed]

Knox, W. H.

Kobat, D.

Kosinski, S. G.

Koulmanda, M.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Lichtman, J. W.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Lin, C. P.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Liu, X.

Livet, J.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Lu, J.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Lu, Y.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Luan, F.

D. V. Skryabin, F. Luan, J. C. Knight, and P. S. Russell, “Soliton self-frequency shift cancellation in photonic crystal fibers,” Science 301(5640), 1705–1708 (2003).
[CrossRef] [PubMed]

Lütgehetmann, M.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Makarov, N. S.

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

Moossa, A. R.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Niebuhr, B.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Nishimura, N.

Nishizawa, N.

N. Nishizawa, R. Okamura, and T. Goto, “Simultaneous generation of wavelength tunable two-colored femtosecond soliton pulses using optical fibers,” IEEE Photon. Technol. Lett. 11(4), 421–423 (1999).
[CrossRef]

N. Nishizawa and T. Goto, “Compact system of wavelength-tunable femtosecond soliton pulse generation using optical fibers,” IEEE Photon. Technol. Lett. 11(3), 325–327 (1999).
[CrossRef]

Okamura, R.

N. Nishizawa, R. Okamura, and T. Goto, “Simultaneous generation of wavelength tunable two-colored femtosecond soliton pulses using optical fibers,” IEEE Photon. Technol. Lett. 11(4), 421–423 (1999).
[CrossRef]

Pitsillides, C. M.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Pollard, J. W.

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Pollok, J. M.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Prasher, D. C.

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
[CrossRef] [PubMed]

Rebane, A.

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

Roussos, E. T.

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Russell, P. S.

D. V. Skryabin, F. Luan, J. C. Knight, and P. S. Russell, “Soliton self-frequency shift cancellation in photonic crystal fibers,” Science 301(5640), 1705–1708 (2003).
[CrossRef] [PubMed]

Sanes, J. R.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Schaffer, C. B.

Singh, G.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Skryabin, D. V.

D. V. Skryabin, F. Luan, J. C. Knight, and P. S. Russell, “Soliton self-frequency shift cancellation in photonic crystal fibers,” Science 301(5640), 1705–1708 (2003).
[CrossRef] [PubMed]

Spencer, J. A.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Stocking, C.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[CrossRef] [PubMed]

Strom, T. B.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Täger, M.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Thomaschewski, M.

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Tillo, S. E.

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

Tomita, K.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Toxavidis, V.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Tsuchiya, H.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Tu, Y.

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
[CrossRef] [PubMed]

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[CrossRef] [PubMed]

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K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

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K. Wang and C. Xu, “Tunable high-energy soliton pulse generation from a large-mode-area fiber and its application to third harmonic generation microscopy,” Appl. Phys. Lett. 99(7), 071112 (2011).
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K. Wang and C. Xu, “Wavelength-tunable high-energy soliton pulse generation from a large-mode-area fiber pumped by a time-lens source,” Opt. Lett. 36(6), 942–944 (2011).
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M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
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K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

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W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[CrossRef] [PubMed]

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K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Weissman, T. A.

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Windeler, R. S.

Wong, A. W.

Wyckoff, J. B.

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Xu, C.

Xu, M.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Yamamoto, N.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Yamauchi, K.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Yang, M.

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

Yun, S. H.

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

Appl. Phys. Lett. (1)

K. Wang and C. Xu, “Tunable high-energy soliton pulse generation from a large-mode-area fiber and its application to third harmonic generation microscopy,” Appl. Phys. Lett. 99(7), 071112 (2011).
[CrossRef]

Cancer Res. (1)

K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman, “Development of real-time subcellular dynamic multicolor imaging of cancer-cell trafficking in live mice with a variable-magnification whole-mouse imaging system,” Cancer Res. 66(8), 4208–4214 (2006).
[CrossRef] [PubMed]

IEEE Photon. Technol. Lett. (2)

N. Nishizawa, R. Okamura, and T. Goto, “Simultaneous generation of wavelength tunable two-colored femtosecond soliton pulses using optical fibers,” IEEE Photon. Technol. Lett. 11(4), 421–423 (1999).
[CrossRef]

N. Nishizawa and T. Goto, “Compact system of wavelength-tunable femtosecond soliton pulse generation using optical fibers,” IEEE Photon. Technol. Lett. 11(3), 325–327 (1999).
[CrossRef]

Nat. Med. (2)

Z. Fan, J. A. Spencer, Y. Lu, C. M. Pitsillides, G. Singh, P. Kim, S. H. Yun, V. Toxavidis, T. B. Strom, C. P. Lin, and M. Koulmanda, “In vivo tracking of ‘color-coded’ effector, natural and induced regulatory T cells in the allograft response,” Nat. Med. 16(6), 718–722 (2010).
[CrossRef] [PubMed]

K. Weber, M. Thomaschewski, M. Warlich, T. Volz, K. Cornils, B. Niebuhr, M. Täger, M. Lütgehetmann, J. M. Pollok, C. Stocking, M. Dandri, D. Benten, and B. Fehse, “RGB marking facilitates multicolor clonal cell tracking,” Nat. Med. 17(4), 504–509 (2011).
[CrossRef] [PubMed]

Nat. Methods (2)

K. Hope and M. Bhatia, “Clonal interrogation of stem cells,” Nat. Methods 8(4Suppl), S36–S40 (2011).
[CrossRef] [PubMed]

M. Drobizhev, N. S. Makarov, S. E. Tillo, T. E. Hughes, and A. Rebane, “Two-photon absorption properties of fluorescent proteins,” Nat. Methods 8(5), 393–399 (2011).
[CrossRef] [PubMed]

Nat. Protoc. (1)

D. Entenberg, J. B. Wyckoff, B. Gligorijevic, E. T. Roussos, V. V. Verkhusha, J. W. Pollard, and J. Condeelis, “Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging,” Nat. Protoc. 6(10), 1500–1520 (2011).
[CrossRef] [PubMed]

Nature (1)

J. Livet, T. A. Weissman, H. Kang, R. W. Draft, J. Lu, R. A. Bennis, J. R. Sanes, and J. W. Lichtman, “Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system,” Nature 450(7166), 56–62 (2007).
[CrossRef] [PubMed]

Opt. Express (1)

Opt. Lett. (3)

Science (3)

M. Chalfie, Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher, “Green fluorescent protein as a marker for gene expression,” Science 263(5148), 802–805 (1994).
[CrossRef] [PubMed]

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[CrossRef] [PubMed]

D. V. Skryabin, F. Luan, J. C. Knight, and P. S. Russell, “Soliton self-frequency shift cancellation in photonic crystal fibers,” Science 301(5640), 1705–1708 (2003).
[CrossRef] [PubMed]

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Figures (4)

Fig. 1
Fig. 1

Experimental setup. HWP: half-wave plate, L1~L8: lenses, M: mirror, DC1: 1800 nm dichroic, DC2: 900 nm dichroic, DC3: 801 nm dichroic, LMA fiber: PM LMA35 fiber.

Fig. 2
Fig. 2

(a) Soliton spectra (in log scale) after the LMA fiber. Second-order interferometric autocorrelation traces for the solitons at 1728 nm (b) and 1900 nm (c).

Fig. 3
Fig. 3

SH spectra at 775 nm (a), 864 nm (b) and 950 nm (c). The corresponding second-order interferometric autocorrelation traces for 775 nm (d), 864 nm (e) and 950 nm (f).

Fig. 4
Fig. 4

2PM fluorescence images of (a) TagCFP, (b) TagYFP, and (c) TagRFP cell lines excited separately at 864 nm (first rows), 950 nm (second rows), and 775 nm (third rows). Three detection channels have bandpass filters of 420-500nm (first columns), 500-550nm (second columns), and 584-676 nm (third columns). (d) Simultaneous 2PM images of A375-Rainbow cells excited by the three-color femtosecond lasers. Calibrated from (a-c), signals from TagCFP (cyan), TagYFP (yellow), and TagRFP (red) channels are combined into a color coded image [bottom right one in (d)], representing cells expressing different colors.

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