Abstract

The design and fabrication of custom-tailored microarrays for use as phantoms in the characterization of hyperspectral imaging systems is described. Corresponding analysis methods for biologically relevant samples are also discussed. An image-based phantom design was used to program a microarrayer robot to print prescribed mixtures of dyes onto microscope slides. The resulting arrays were imaged by a hyperspectral imaging microscope. The shape of the spots results in significant scattering signals, which can be used to test image analysis algorithms. Separation of the scattering signals allowed elucidation of individual dye spectra. In addition, spectral fitting of the absorbance spectra of complex dye mixtures was performed in order to determine local dye concentrations. Such microarray phantoms provide a robust testing platform for comparisons of hyperspectral imaging acquisition and analysis methods.

© 2012 OSA

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    [CrossRef] [PubMed]
  18. G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
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  20. W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
    [CrossRef]

2012 (1)

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

2011 (2)

M. L. Clarke, M. Litorja, D. W. Allen, D. V. Samarov, and J. Hwang, “Characterization of hyperspectral imaging and analysis via microarray printing of dyes,” Proc. SPIE7891, 78910W (2011).
[CrossRef]

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

2009 (1)

W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
[CrossRef]

2008 (3)

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

J. W. Qin and R. F. Lu, “Measurement of the optical properties of fruits and vegetables using spatially resolved hyperspectral diffuse reflectance imaging technique,” Postharvest Biol. Technol.49(3), 355–365 (2008).
[CrossRef]

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

2007 (5)

W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, “Snapshot hyperspectral imaging in ophthalmology,” J. Biomed. Opt.12(1), 014036 (2007).
[CrossRef] [PubMed]

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

C. Glasenapp, W. Mönch, H. Krause, and H. Zappe, “Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection,” J. Biomed. Opt.12(1), 014038 (2007).
[CrossRef] [PubMed]

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

A. Bricaud, C. Mejia, D. Blondeau-Patissier, H. Claustre, M. Crepon, and S. Thiria, “Retrieval of pigment concentrations and size structure of algal populations from their absorption spectra using multilayered perceptrons,” Appl. Opt.46(8), 1251–1260 (2007).
[CrossRef] [PubMed]

2006 (1)

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

2005 (2)

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt.10(4), 044004 (2005).
[CrossRef] [PubMed]

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

2004 (2)

2003 (1)

K. J. Zuzak, M. T. Gladwin, R. O. Cannon, and I. W. Levin, “Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide,” Am. J. Physiol. Heart Circ. Physiol.285(3), H1183–H1189 (2003).
[PubMed]

2002 (1)

M. L. Huebschman, R. A. Schultz, and H. R. Garner, “Characteristics and capabilities of the hyperspectral imaging microscope,” IEEE Eng. Med. Biol. Mag.21(4), 104–117 (2002).
[CrossRef] [PubMed]

1996 (1)

R. Richards-Kortum and E. Sevick-Muraca, “Quantitative optical spectroscopy for tissue diagnosis,” Annu. Rev. Phys. Chem.47(1), 555–606 (1996).
[CrossRef] [PubMed]

Allen, D. W.

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

M. L. Clarke, M. Litorja, D. W. Allen, D. V. Samarov, and J. Hwang, “Characterization of hyperspectral imaging and analysis via microarray printing of dyes,” Proc. SPIE7891, 78910W (2011).
[CrossRef]

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Andersson, C.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Aoki, H.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Bassukas, I.

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

Bearman, G.

W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, “Snapshot hyperspectral imaging in ophthalmology,” J. Biomed. Opt.12(1), 014036 (2007).
[CrossRef] [PubMed]

Bigio, I.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Blondeau-Patissier, D.

Bricaud, A.

Burger, J.

P. Geladi, J. Burger, and T. Lestander, “Hyperspectral imaging: calibration problems and solutions,” Chemom. Intell. Lab. Syst.72(2), 209–217 (2004).
[CrossRef]

Cannon, R. O.

K. J. Zuzak, M. T. Gladwin, R. O. Cannon, and I. W. Levin, “Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide,” Am. J. Physiol. Heart Circ. Physiol.285(3), H1183–H1189 (2003).
[PubMed]

Cao, Y. T.

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt.10(4), 044004 (2005).
[CrossRef] [PubMed]

Chang, R.

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Chao, K.

W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
[CrossRef]

Chen, K.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Cipolloni, P. B.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Clarke, M. L.

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

M. L. Clarke, M. Litorja, D. W. Allen, D. V. Samarov, and J. Hwang, “Characterization of hyperspectral imaging and analysis via microarray printing of dyes,” Proc. SPIE7891, 78910W (2011).
[CrossRef]

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Claustre, H.

Crepon, M.

Cunningham, G.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Denovo, R. C.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Dewhirst, M. W.

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt.10(4), 044004 (2005).
[CrossRef] [PubMed]

Dimou, A.

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

Dinh, T.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Donovan, O.

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt.10(4), 044004 (2005).
[CrossRef] [PubMed]

Erfurth, F.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Fang, H.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Fassler, D.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Fink, W.

W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, “Snapshot hyperspectral imaging in ophthalmology,” J. Biomed. Opt.12(1), 014036 (2007).
[CrossRef] [PubMed]

Freedman, S. D.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Freeman, J.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Fujikado, T.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Galaris, D.

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

Garner, H. R.

M. L. Huebschman, R. A. Schultz, and H. R. Garner, “Characteristics and capabilities of the hyperspectral imaging microscope,” IEEE Eng. Med. Biol. Mag.21(4), 104–117 (2002).
[CrossRef] [PubMed]

Geladi, P.

P. Geladi, J. Burger, and T. Lestander, “Hyperspectral imaging: calibration problems and solutions,” Chemom. Intell. Lab. Syst.72(2), 209–217 (2004).
[CrossRef]

Ghiran, I. C.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Giurini, J. M.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Gladwin, M. T.

K. J. Zuzak, M. T. Gladwin, R. O. Cannon, and I. W. Levin, “Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide,” Am. J. Physiol. Heart Circ. Physiol.285(3), H1183–H1189 (2003).
[PubMed]

Glasenapp, C.

C. Glasenapp, W. Mönch, H. Krause, and H. Zappe, “Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection,” J. Biomed. Opt.12(1), 014038 (2007).
[CrossRef] [PubMed]

Greenman, R. L.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Haaland, D. M.

Hanlon, E. B.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Hirohara, Y.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Huebschman, M. L.

M. L. Huebschman, R. A. Schultz, and H. R. Garner, “Characteristics and capabilities of the hyperspectral imaging microscope,” IEEE Eng. Med. Biol. Mag.21(4), 104–117 (2002).
[CrossRef] [PubMed]

Humayun, M.

W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, “Snapshot hyperspectral imaging in ophthalmology,” J. Biomed. Opt.12(1), 014036 (2007).
[CrossRef] [PubMed]

Hwang, J.

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

M. L. Clarke, M. Litorja, D. W. Allen, D. V. Samarov, and J. Hwang, “Characterization of hyperspectral imaging and analysis via microarray printing of dyes,” Proc. SPIE7891, 78910W (2011).
[CrossRef]

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Itzkan, I.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Johnson, W. R.

W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, “Snapshot hyperspectral imaging in ophthalmology,” J. Biomed. Opt.12(1), 014036 (2007).
[CrossRef] [PubMed]

Jun, W.

W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
[CrossRef]

Kasili, P.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Kaxiras, E.

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

Khaodhiar, L.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Kim, M.

W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
[CrossRef]

Kimerer, L. M.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Krause, H.

C. Glasenapp, W. Mönch, H. Krause, and H. Zappe, “Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection,” J. Biomed. Opt.12(1), 014038 (2007).
[CrossRef] [PubMed]

Lee, J. Y.

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Lee, K.

W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
[CrossRef]

Lesoine, J. F.

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Lestander, T.

P. Geladi, J. Burger, and T. Lestander, “Hyperspectral imaging: calibration problems and solutions,” Chemom. Intell. Lab. Syst.72(2), 209–217 (2004).
[CrossRef]

Levin, I. W.

K. J. Zuzak, M. T. Gladwin, R. O. Cannon, and I. W. Levin, “Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide,” Am. J. Physiol. Heart Circ. Physiol.285(3), H1183–H1189 (2003).
[PubMed]

Lim, K. H.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Litorja, M.

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

M. L. Clarke, M. Litorja, D. W. Allen, D. V. Samarov, and J. Hwang, “Characterization of hyperspectral imaging and analysis via microarray printing of dyes,” Proc. SPIE7891, 78910W (2011).
[CrossRef]

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Longoria, L.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Lu, R. F.

J. W. Qin and R. F. Lu, “Measurement of the optical properties of fruits and vegetables using spatially resolved hyperspectral diffuse reflectance imaging technique,” Postharvest Biol. Technol.49(3), 355–365 (2008).
[CrossRef]

Lyons, T. E.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Maeda, N.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Martin, M. E.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Mejia, C.

Mihashi, T.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Millner, P.

W. Jun, M. Kim, K. Lee, P. Millner, and K. Chao, “Assessment of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging,” Sens. Instrum. Food Qual. Saf.3(1), 41–48 (2009).
[CrossRef]

Modell, M.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Moeller, B. J.

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt.10(4), 044004 (2005).
[CrossRef] [PubMed]

Mönch, W.

C. Glasenapp, W. Mönch, H. Krause, and H. Zappe, “Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection,” J. Biomed. Opt.12(1), 014038 (2007).
[CrossRef] [PubMed]

Mrotzek, G.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Nakazawa, N.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Nyuyki, B.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Okawa, Y.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Overholt, B.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Panasyuk, S.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Panjehpour, M.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Perelman, L. T.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Phan, M.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Qin, J. W.

J. W. Qin and R. F. Lu, “Measurement of the optical properties of fruits and vegetables using spatially resolved hyperspectral diffuse reflectance imaging technique,” Postharvest Biol. Technol.49(3), 355–365 (2008).
[CrossRef]

Qiu, L.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Richards-Kortum, R.

R. Richards-Kortum and E. Sevick-Muraca, “Quantitative optical spectroscopy for tissue diagnosis,” Annu. Rev. Phys. Chem.47(1), 555–606 (1996).
[CrossRef] [PubMed]

Sachs, B. P.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Salahuddin, S.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Saluz, H. P.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Samarov, D. V.

D. V. Samarov, M. L. Clarke, J. Y. Lee, D. W. Allen, M. Litorja, and J. Hwang, “Validating the LASSO algorithm by unmixing spectral signatures in multicolor phantoms,” Proc. SPIE8229, 82290Z (2012).
[CrossRef]

M. L. Clarke, M. Litorja, D. W. Allen, D. V. Samarov, and J. Hwang, “Characterization of hyperspectral imaging and analysis via microarray printing of dyes,” Proc. SPIE7891, 78910W (2011).
[CrossRef]

Schmidt, W. D.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Schultz, R. A.

M. L. Huebschman, R. A. Schultz, and H. R. Garner, “Characteristics and capabilities of the hyperspectral imaging microscope,” IEEE Eng. Med. Biol. Mag.21(4), 104–117 (2002).
[CrossRef] [PubMed]

Sevick-Muraca, E.

R. Richards-Kortum and E. Sevick-Muraca, “Quantitative optical spectroscopy for tissue diagnosis,” Annu. Rev. Phys. Chem.47(1), 555–606 (1996).
[CrossRef] [PubMed]

Sinclair, M. B.

Sorg, B. S.

B. S. Sorg, B. J. Moeller, O. Donovan, Y. T. Cao, and M. W. Dewhirst, “Hyperspectral imaging of hemoglobin saturation in tumor microvasculature and tumor hypoxia development,” J. Biomed. Opt.10(4), 044004 (2005).
[CrossRef] [PubMed]

Thiria, S.

Timlin, J. A.

Tokumasu, F.

J. Y. Lee, M. L. Clarke, F. Tokumasu, J. F. Lesoine, D. W. Allen, R. Chang, M. Litorja, and J. Hwang, “Absorption-based hyperspectral imaging and analysis of single erythrocytes,” IEEE J. Sel. Topics Quantum Electron.17(6) 1801–1840 (2011).

Tretyakov, A.

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Tsolakidis, A.

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

Tsuruga, Y.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Uchida, I.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Veves, A.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Vitkin, E.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Vo-Dinh, T.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Wabuyele, M. B.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Wang, X.

R. L. Greenman, S. Panasyuk, X. Wang, T. E. Lyons, T. Dinh, L. Longoria, J. M. Giurini, J. Freeman, L. Khaodhiar, and A. Veves, “Early changes in the skin microcirculation and muscle metabolism of the diabetic foot,” Lancet366(9498), 1711–1717 (2005).
[CrossRef] [PubMed]

Werner-Washburne, M.

Wilson, D.

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Wilson, D. W.

W. R. Johnson, D. W. Wilson, W. Fink, M. Humayun, and G. Bearman, “Snapshot hyperspectral imaging in ophthalmology,” J. Biomed. Opt.12(1), 014036 (2007).
[CrossRef] [PubMed]

Yamaguchi, T.

Y. Hirohara, Y. Okawa, T. Mihashi, T. Yamaguchi, N. Nakazawa, Y. Tsuruga, H. Aoki, N. Maeda, I. Uchida, and T. Fujikado, “Validity of retinal oxygen saturation analysis: Hyperspectral imaging in visible wavelength with fundus camera and liquid crystal wavelength tunable filter,” Opt. Rev.14(3), 151–158 (2007).
[CrossRef]

Zaman, M. M.

I. Itzkan, L. Qiu, H. Fang, M. M. Zaman, E. Vitkin, I. C. Ghiran, S. Salahuddin, M. Modell, C. Andersson, L. M. Kimerer, P. B. Cipolloni, K. H. Lim, S. D. Freedman, I. Bigio, B. P. Sachs, E. B. Hanlon, and L. T. Perelman, “Confocal light absorption and scattering spectroscopic microscopy monitors organelles in live cells with no exogenous labels,” Proc. Natl. Acad. Sci. U.S.A.104(44), 17255–17260 (2007).
[CrossRef] [PubMed]

Zappe, H.

C. Glasenapp, W. Mönch, H. Krause, and H. Zappe, “Biochip reader with dynamic holographic excitation and hyperspectral fluorescence detection,” J. Biomed. Opt.12(1), 014038 (2007).
[CrossRef] [PubMed]

Zonios, G.

G. Zonios, A. Dimou, I. Bassukas, D. Galaris, A. Tsolakidis, and E. Kaxiras, “Melanin absorption spectroscopy: new method for noninvasive skin investigation and melanoma detection,” J. Biomed. Opt.13(1), 014017 (2008).
[CrossRef] [PubMed]

Zuzak, K. J.

K. J. Zuzak, M. T. Gladwin, R. O. Cannon, and I. W. Levin, “Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide,” Am. J. Physiol. Heart Circ. Physiol.285(3), H1183–H1189 (2003).
[PubMed]

Am. J. Physiol. Heart Circ. Physiol. (1)

K. J. Zuzak, M. T. Gladwin, R. O. Cannon, and I. W. Levin, “Imaging hemoglobin oxygen saturation in sickle cell disease patients using noninvasive visible reflectance hyperspectral techniques: effects of nitric oxide,” Am. J. Physiol. Heart Circ. Physiol.285(3), H1183–H1189 (2003).
[PubMed]

Anal. Chem. (1)

F. Erfurth, A. Tretyakov, B. Nyuyki, G. Mrotzek, W. D. Schmidt, D. Fassler, and H. P. Saluz, “Two-laser, large-field hyperspectral microarray scanner for the analysis of multicolor microarrays,” Anal. Chem.80(20), 7706–7713 (2008).
[CrossRef] [PubMed]

Ann. Biomed. Eng. (1)

M. E. Martin, M. B. Wabuyele, K. Chen, P. Kasili, M. Panjehpour, M. Phan, B. Overholt, G. Cunningham, D. Wilson, R. C. Denovo, and T. Vo-Dinh, “Development of an advanced hyperspectral imaging (HSI) system with applications for cancer detection,” Ann. Biomed. Eng.34(6), 1061–1068 (2006).
[CrossRef] [PubMed]

Annu. Rev. Phys. Chem. (1)

R. Richards-Kortum and E. Sevick-Muraca, “Quantitative optical spectroscopy for tissue diagnosis,” Annu. Rev. Phys. Chem.47(1), 555–606 (1996).
[CrossRef] [PubMed]

Appl. Opt. (2)

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Supplementary Material (1)

» Media 1: PDF (26 KB)     

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Figures (5)

Fig. 1
Fig. 1

(a) A printing design is chosen or created in a program such as Photoshop. Here the U.S. Department of Commerce logo, containing 5 colors, is shown in a reduced image size and consists of five colors. (b) The colors represent printing wells (four of which correspond to four distinct colors, with white indicating that a spot should not be printed at that location). (c) The image and well codes are fed into MATLAB, which generates the appropriate code to drive the SpotBot II (see Media 1), as shown here. (d) The image was printed on Epson glossy photo paper to illustrate the printing procedure by showing the spots macroscopically. (e) A close-up comparison of a region of the inputted image and its corresponding printed image.

Fig. 2
Fig. 2

Examples of detected images from the hyperspectral data cubes of a printed spot on glass. Signal and dark signal intensities are shown for the glass background datacube (Io), and the sample datacube (I). These intensities are used to calculate the absorbance image A. All images are from the same spatial location on the CCD (i.e., the same x,y coordinates). Ring patterns within the hemispherical PEG-based spot are caused by scattering and refraction of the illumination light.

Fig. 3
Fig. 3

(a) A pattern designed in Photoshop (shown with identifying notations), was used to generate a test microarray that consists of PEG spots containing varying amounts of three spectrally dissimilar dyes (AR, BBR, EG). (b) This sample was imaged by a hyperspectral microscope (shown here for 695 nm illumination). Scalebar is 200 μm. (c) The absorption spectra of dye spots measured by the hyperspectral microscope are shown before (top) and after (bottom) subtraction of the PEG background.

Fig. 4
Fig. 4

The absorbance spectra of two pure but spectrally similar dyes (AR and NC) and the mean PEG signal, obtained from a microarray phantom.

Fig. 5
Fig. 5

(a) Spectral data collected from the microarray shown in Fig. 3 are displayed along with fitted spectra, obtained by a linear combination method. (b) The same process, applied to two dyes with similar absorption profiles. The number after the name of a dye indicates the percentage of the dye relative to the maximum printed solution concentration.

Tables (1)

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Table 1 Expected and calculated concentrations from linear mixing analysis of two dyes: AR and NC. Concentrations are relative to the maximum printed solution concentration. Standard deviation is calculated from the analysis of three different microarrays on the same slide.

Equations (2)

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A x,y,λ = log 10 I x,y,λ I dark,x,y,λ I o,x,y,λ I o,dark,x,y,λ .
A fit,λ = c AR A AR100,λ + c NC A NC100,λ + A PEG,λ +offset,

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