Abstract

Waveguide numerical aperture restrictions and light-blocking elements are used to create a microfluidic cytometer with both illumination and two-parameter light scatter collection systems integrated on-chip. Good forward scatter coefficients of variation (9.7-18.3%) are achieved for polystyrene beads under a reasonably high flow rate (28 cm/s) using a greatly simplified optical system.

© 2010 OSA

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
  4. A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
    [CrossRef] [PubMed]
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  6. N. Pamme, R. Koyama, and A. Manz, “Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay,” Lab Chip 3(3), 187–192 (2003).
    [CrossRef] [PubMed]
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2009 (3)

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

2006 (1)

J. Godin, V. Lien, and Y. Lo, “Demonstration of two-dimensional fluidic lens for integration into microfluidic flow cytometers,” Appl. Phys. Lett. 89(6), 061106 (2006).
[CrossRef]

2004 (3)

V. Lien, Y. Berdichevsky, and Y. Lo, “A prealigned process of integrating optical waveguides with microfluidic devices,” IEEE Photon. Technol. Lett. 16(6), 1525–1527 (2004).
[CrossRef]

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

J. Seo and L. P. Lee, “Disposable integrated microfluidics with self-aligned planar microlenses,” Sens. Actuators B Chem. 99(2-3), 615–622 (2004).
[CrossRef]

2003 (2)

N. Pamme, R. Koyama, and A. Manz, “Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay,” Lab Chip 3(3), 187–192 (2003).
[CrossRef] [PubMed]

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

1999 (1)

D. P. Schrum, C. Culbertson, S. Jacobson, and J. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Anal. Chem. 71(19), 4173–4177 (1999).
[CrossRef]

1998 (1)

D. Duffy, J. McDonald, O. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly (dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998).
[CrossRef]

1990 (1)

H. B. Steen, “Light scattering measurement in an arc lamp-based flow cytometer,” Cytometry 11(2), 223–230 (1990).
[CrossRef] [PubMed]

Anderson, G. P.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Berdichevsky, Y.

V. Lien, Y. Berdichevsky, and Y. Lo, “A prealigned process of integrating optical waveguides with microfluidic devices,” IEEE Photon. Technol. Lett. 16(6), 1525–1527 (2004).
[CrossRef]

Brattke, K.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Chen, C. H.

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

Cho, S. H.

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

Culbertson, C.

D. P. Schrum, C. Culbertson, S. Jacobson, and J. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Anal. Chem. 71(19), 4173–4177 (1999).
[CrossRef]

Duffy, D.

D. Duffy, J. McDonald, O. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly (dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998).
[CrossRef]

El-Ali, J.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

Engelund, M.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

Erickson, J. S.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Erten, A.

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

Frankowski, M.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Friis, P.

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Godin, J.

J. Godin, V. Lien, and Y. Lo, “Demonstration of two-dimensional fluidic lens for integration into microfluidic flow cytometers,” Appl. Phys. Lett. 89(6), 061106 (2006).
[CrossRef]

Golden, J. P.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Goranovic, G.

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Gotsaed, T.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

Hilliard, L. R.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Howell, P. B.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Jacobson, S.

D. P. Schrum, C. Culbertson, S. Jacobson, and J. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Anal. Chem. 71(19), 4173–4177 (1999).
[CrossRef]

Kim, J. S.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Koyama, R.

N. Pamme, R. Koyama, and A. Manz, “Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay,” Lab Chip 3(3), 187–192 (2003).
[CrossRef] [PubMed]

Kummrow, A.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Kutter, J. P.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Larsen, U. D.

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Lee, L. P.

J. Seo and L. P. Lee, “Disposable integrated microfluidics with self-aligned planar microlenses,” Sens. Actuators B Chem. 99(2-3), 615–622 (2004).
[CrossRef]

Lien, V.

J. Godin, V. Lien, and Y. Lo, “Demonstration of two-dimensional fluidic lens for integration into microfluidic flow cytometers,” Appl. Phys. Lett. 89(6), 061106 (2006).
[CrossRef]

V. Lien, Y. Berdichevsky, and Y. Lo, “A prealigned process of integrating optical waveguides with microfluidic devices,” IEEE Photon. Technol. Lett. 16(6), 1525–1527 (2004).
[CrossRef]

Ligler, F. S.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Lo, Y.

J. Godin, V. Lien, and Y. Lo, “Demonstration of two-dimensional fluidic lens for integration into microfluidic flow cytometers,” Appl. Phys. Lett. 89(6), 061106 (2006).
[CrossRef]

V. Lien, Y. Berdichevsky, and Y. Lo, “A prealigned process of integrating optical waveguides with microfluidic devices,” IEEE Photon. Technol. Lett. 16(6), 1525–1527 (2004).
[CrossRef]

Lo, Y. H.

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

Manz, A.

N. Pamme, R. Koyama, and A. Manz, “Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay,” Lab Chip 3(3), 187–192 (2003).
[CrossRef] [PubMed]

McDonald, J.

D. Duffy, J. McDonald, O. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly (dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998).
[CrossRef]

Mogensen, K. B.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

Nasir, M.

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

Neukammer, J.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Pamme, N.

N. Pamme, R. Koyama, and A. Manz, “Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay,” Lab Chip 3(3), 187–192 (2003).
[CrossRef] [PubMed]

Perch-Nielsen, I. R.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Poulsen, C. R.

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Ramsey, J.

D. P. Schrum, C. Culbertson, S. Jacobson, and J. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Anal. Chem. 71(19), 4173–4177 (1999).
[CrossRef]

Schmidt, M.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Schrum, D. P.

D. P. Schrum, C. Culbertson, S. Jacobson, and J. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Anal. Chem. 71(19), 4173–4177 (1999).
[CrossRef]

Schueller, O.

D. Duffy, J. McDonald, O. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly (dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998).
[CrossRef]

Seo, J.

J. Seo and L. P. Lee, “Disposable integrated microfluidics with self-aligned planar microlenses,” Sens. Actuators B Chem. 99(2-3), 615–622 (2004).
[CrossRef]

Snakenborg, D.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

Steen, H. B.

H. B. Steen, “Light scattering measurement in an arc lamp-based flow cytometer,” Cytometry 11(2), 223–230 (1990).
[CrossRef] [PubMed]

Telleman, P.

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Theisen, J.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Tsai, F.

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

Tuchscheerer, A.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Wang, Z.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

Whitesides, G. M.

D. Duffy, J. McDonald, O. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly (dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998).
[CrossRef]

Wolff, A.

Z. Wang, J. El-Ali, M. Engelund, T. Gotsaed, I. R. Perch-Nielsen, K. B. Mogensen, D. Snakenborg, J. P. Kutter, and A. Wolff, “Measurements of scattered light on a microchip flow cytometer with integrated polymer based optical elements,” Lab Chip 4(4), 372–377 (2004).
[CrossRef] [PubMed]

A. Wolff, I. R. Perch-Nielsen, U. D. Larsen, P. Friis, G. Goranovic, C. R. Poulsen, J. P. Kutter, and P. Telleman, “Integrating advanced functionality in a microfabricated high-throughput fluorescent-activated cell sorter,” Lab Chip 3(1), 22–27 (2003).
[CrossRef] [PubMed]

Yildirim, H.

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

Anal. Chem. (2)

D. P. Schrum, C. Culbertson, S. Jacobson, and J. Ramsey, “Microchip flow cytometry using electrokinetic focusing,” Anal. Chem. 71(19), 4173–4177 (1999).
[CrossRef]

D. Duffy, J. McDonald, O. Schueller, and G. M. Whitesides, “Rapid prototyping of microfluidic systems in poly (dimethylsiloxane),” Anal. Chem. 70(23), 4974–4984 (1998).
[CrossRef]

Appl. Phys. Lett. (1)

J. Godin, V. Lien, and Y. Lo, “Demonstration of two-dimensional fluidic lens for integration into microfluidic flow cytometers,” Appl. Phys. Lett. 89(6), 061106 (2006).
[CrossRef]

Biomed. Microdevices (1)

C. H. Chen, S. H. Cho, F. Tsai, A. Erten, and Y. H. Lo, “Microfluidic cell sorter with integrated piezoelectric actuator,” Biomed. Microdevices 11(6), 1223–1231 (2009).
[CrossRef] [PubMed]

Cytometry (1)

H. B. Steen, “Light scattering measurement in an arc lamp-based flow cytometer,” Cytometry 11(2), 223–230 (1990).
[CrossRef] [PubMed]

IEEE Photon. Technol. Lett. (1)

V. Lien, Y. Berdichevsky, and Y. Lo, “A prealigned process of integrating optical waveguides with microfluidic devices,” IEEE Photon. Technol. Lett. 16(6), 1525–1527 (2004).
[CrossRef]

Lab Chip (5)

A. Kummrow, J. Theisen, M. Frankowski, A. Tuchscheerer, H. Yildirim, K. Brattke, M. Schmidt, and J. Neukammer, “Microfluidic structures for flow cytometric analysis of hydrodynamically focussed blood cells fabricated by ultraprecision micromachining,” Lab Chip 9(7), 972–981 (2009).
[CrossRef] [PubMed]

J. P. Golden, J. S. Kim, J. S. Erickson, L. R. Hilliard, P. B. Howell, G. P. Anderson, M. Nasir, and F. S. Ligler, “Multi-wavelength microflow cytometer using groove-generated sheath flow,” Lab Chip 9(13), 1942–1950 (2009).
[CrossRef] [PubMed]

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