Abstract

It has been demonstrated that the presence of cancer results in detectable changes to uninvolved tissues, collectively termed cancer field effects (CFE). In this study, we directly assessed the ability of Raman microspectroscopy to detect CFE via in-vitro study of organotypic tissue rafts approximating human skin. Raman spectra were measured from both epidermis and dermis after transfer of the rafts to dishes containing adherent cultures of either normal human fibroblasts or fibrosarcoma (HT1080) cells. Principal components analyses allowed discrimination between the groups with 86% classification accuracy in the epidermis and 94% in the dermis. These results encourage further study to evaluate the Raman capacity for detecting CFE as a possible tool for noninvasive screening for tumor presence.

© 2010 OSA

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2008 (3)

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

2007 (1)

2006 (2)

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

2005 (1)

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

2004 (2)

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

2003 (4)

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

O. De Wever and M. Mareel, “Role of tissue stroma in cancer cell invasion,” J. Pathol. 200(4), 429–447 (2003).
[CrossRef] [PubMed]

A. Robichaux Viehoever, D. Anderson, D. Jansen, and A. Mahadevan-Jansen, “Organotypic raft cultures as an effective in vitro tool for understanding Raman spectral analysis of tissue,” Photochem. Photobiol. 78(5), 517–524 (2003).
[CrossRef] [PubMed]

C. A. Lieber and A. Mahadevan-Jansen, “Automated method for subtraction of fluorescence from biological Raman spectra,” Appl. Spectrosc. 57(11), 1363–1367 (2003).
[CrossRef] [PubMed]

1995 (2)

H. G. M. Edwards, A. C. Williams, and B. W. Barry, “Potential applications of FT-Raman spectroscopy for dermatological diagnostics,” J. Mol. Struct. 347, 379–387 (1995).
[CrossRef]

C. J. Frank, R. L. McCreery, and D. C. Redd, “Raman spectroscopy of normal and diseased human breast tissues,” Anal. Chem. 67(5), 777–783 (1995).
[CrossRef] [PubMed]

Anderson, D.

A. Robichaux Viehoever, D. Anderson, D. Jansen, and A. Mahadevan-Jansen, “Organotypic raft cultures as an effective in vitro tool for understanding Raman spectral analysis of tissue,” Photochem. Photobiol. 78(5), 517–524 (2003).
[CrossRef] [PubMed]

Backman, V.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Barry, B. W.

H. G. M. Edwards, A. C. Williams, and B. W. Barry, “Potential applications of FT-Raman spectroscopy for dermatological diagnostics,” J. Mol. Struct. 347, 379–387 (1995).
[CrossRef]

Beaven, R. B.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Billheimer, D.

Billheimer, D. D.

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

Brand, R. E.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Crowe, J. P.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Dasari, R. R.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

De Wever, O.

O. De Wever and M. Mareel, “Role of tissue stroma in cancer cell invasion,” J. Pathol. 200(4), 429–447 (2003).
[CrossRef] [PubMed]

Edwards, H. G. M.

H. G. M. Edwards, A. C. Williams, and B. W. Barry, “Potential applications of FT-Raman spectroscopy for dermatological diagnostics,” J. Mol. Struct. 347, 379–387 (1995).
[CrossRef]

Ellis, D. L.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

English, J.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

Feld, M. S.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Fitzmaurice, M.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Frank, C. J.

C. J. Frank, R. L. McCreery, and D. C. Redd, “Raman spectroscopy of normal and diseased human breast tissues,” Anal. Chem. 67(5), 777–783 (1995).
[CrossRef] [PubMed]

Galindo, L. H.

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Gandhi, S. J.

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Gardecki, J. A.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Gillbro, J. M.

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Goldberg, M. J.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Gomes, A.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Haka, A. S.

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Hasabou, N.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Hasse, S.

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Hicks, D.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Huang, Z.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

Hutchings, J.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Jameel, M.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Jansen, D.

A. Robichaux Viehoever, D. Anderson, D. Jansen, and A. Mahadevan-Jansen, “Organotypic raft cultures as an effective in vitro tool for understanding Raman spectral analysis of tissue,” Photochem. Photobiol. 78(5), 517–524 (2003).
[CrossRef] [PubMed]

Jones, H.

Kanter, E.

Kanter, E. M.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Keller, M. D.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Kim, Y. L.

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Koetsier, J.

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

Kramer, J. R.

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Kromine, A.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Kromine, A. K.

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Kunte, D. P.

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

Lam, S.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

Lieber, C. A.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

C. A. Lieber and A. Mahadevan-Jansen, “Automated method for subtraction of fluorescence from biological Raman spectra,” Appl. Spectrosc. 57(11), 1363–1367 (2003).
[CrossRef] [PubMed]

Liu, Y.

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Lui, H.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

Lyons, J.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Mahadevan-Jansen, A.

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

A. Robichaux-Viehoever, E. Kanter, H. Shappell, D. Billheimer, H. Jones, and A. Mahadevan-Jansen, “Characterization of Raman spectra measured in vivo for the detection of cervical dysplasia,” Appl. Spectrosc. 61(9), 986–993 (2007).
[CrossRef] [PubMed]

A. Robichaux Viehoever, D. Anderson, D. Jansen, and A. Mahadevan-Jansen, “Organotypic raft cultures as an effective in vitro tool for understanding Raman spectral analysis of tissue,” Photochem. Photobiol. 78(5), 517–524 (2003).
[CrossRef] [PubMed]

C. A. Lieber and A. Mahadevan-Jansen, “Automated method for subtraction of fluorescence from biological Raman spectra,” Appl. Spectrosc. 57(11), 1363–1367 (2003).
[CrossRef] [PubMed]

Majumder, S. K.

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Mareel, M.

O. De Wever and M. Mareel, “Role of tissue stroma in cancer cell invasion,” J. Pathol. 200(4), 429–447 (2003).
[CrossRef] [PubMed]

McCreery, R. L.

C. J. Frank, R. L. McCreery, and D. C. Redd, “Raman spectroscopy of normal and diseased human breast tissues,” Anal. Chem. 67(5), 777–783 (1995).
[CrossRef] [PubMed]

McLean, D. I.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

McWilliams, A.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

Miller, A.

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Moore, J.

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Motz, J. T.

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

Nazemi, J.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Redd, D. C.

C. J. Frank, R. L. McCreery, and D. C. Redd, “Raman spectroscopy of normal and diseased human breast tissues,” Anal. Chem. 67(5), 777–783 (1995).
[CrossRef] [PubMed]

Robichaux Viehoever, A.

A. Robichaux Viehoever, D. Anderson, D. Jansen, and A. Mahadevan-Jansen, “Organotypic raft cultures as an effective in vitro tool for understanding Raman spectral analysis of tissue,” Photochem. Photobiol. 78(5), 517–524 (2003).
[CrossRef] [PubMed]

Robichaux-Viehoever, A.

Rogers, J.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Rokos, H.

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Roy, H. K.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Ruderman, S.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Schallreuter, K. U.

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Shappell, H.

Stone, N.

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Turzhitsky, V.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Vakil, P.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Volynskaya, Z.

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Wali, R. K.

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

Williams, A. C.

H. G. M. Edwards, A. C. Williams, and B. W. Barry, “Potential applications of FT-Raman spectroscopy for dermatological diagnostics,” J. Mol. Struct. 347, 379–387 (1995).
[CrossRef]

Wood, J. M.

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Young, K. L.

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Zeng, H.

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

Anal. Chem. (1)

C. J. Frank, R. L. McCreery, and D. C. Redd, “Raman spectroscopy of normal and diseased human breast tissues,” Anal. Chem. 67(5), 777–783 (1995).
[CrossRef] [PubMed]

Appl. Spectrosc. (2)

Cancer Epidemiol. Biomarkers Prev. (1)

H. K. Roy, Y. L. Kim, R. K. Wali, Y. Liu, J. Koetsier, D. P. Kunte, M. J. Goldberg, and V. Backman, “Spectral markers in preneoplastic intestinal mucosa: an accurate predictor of tumor risk in the MIN mouse,” Cancer Epidemiol. Biomarkers Prev. 14(7), 1639–1645 (2005).
[CrossRef] [PubMed]

Cancer Res. (1)

A. S. Haka, Z. Volynskaya, J. A. Gardecki, J. Nazemi, J. Lyons, D. Hicks, M. Fitzmaurice, R. R. Dasari, J. P. Crowe, and M. S. Feld, “In vivo margin assessment during partial mastectomy breast surgery using raman spectroscopy,” Cancer Res. 66(6), 3317–3322 (2006).
[CrossRef] [PubMed]

Dis. Markers (1)

M. D. Keller, E. M. Kanter, C. A. Lieber, S. K. Majumder, J. Hutchings, D. L. Ellis, R. B. Beaven, N. Stone, and A. Mahadevan-Jansen, “Detecting temporal and spatial effects of epithelial cancers with Raman spectroscopy,” Dis. Markers 25(6), 323–337 (2008).
[PubMed]

Gastroenterology (2)

H. K. Roy, Y. Liu, R. K. Wali, Y. L. Kim, A. K. Kromine, M. J. Goldberg, and V. Backman, “Four-dimensional elastic light-scattering fingerprints as preneoplastic markers in the rat model of colon carcinogenesis,” Gastroenterology 126(4), 1071–1081, discussion 948 (2004).
[CrossRef] [PubMed]

H. K. Roy, A. Gomes, V. Turzhitsky, M. J. Goldberg, J. Rogers, S. Ruderman, K. L. Young, A. Kromine, R. E. Brand, M. Jameel, P. Vakil, N. Hasabou, and V. Backman, “Spectroscopic microvascular blood detection from the endoscopically normal colonic mucosa: biomarker for neoplasia risk,” Gastroenterology 135(4), 1069–1078 (2008).
[CrossRef] [PubMed]

Int. J. Oncol. (1)

Z. Huang, A. McWilliams, S. Lam, J. English, D. I. McLean, H. Lui, and H. Zeng, “Effect of formalin fixation on the near-infrared Raman spectroscopy of normal and cancerous human bronchial tissues,” Int. J. Oncol. 23(3), 649–655 (2003).
[PubMed]

J. Biomed. Opt. (1)

J. T. Motz, M. Fitzmaurice, A. Miller, S. J. Gandhi, A. S. Haka, L. H. Galindo, R. R. Dasari, J. R. Kramer, and M. S. Feld, “In vivo Raman spectral pathology of human atherosclerosis and vulnerable plaque,” J. Biomed. Opt. 11(2), 021003 (2006).
[CrossRef] [PubMed]

J. Mol. Struct. (1)

H. G. M. Edwards, A. C. Williams, and B. W. Barry, “Potential applications of FT-Raman spectroscopy for dermatological diagnostics,” J. Mol. Struct. 347, 379–387 (1995).
[CrossRef]

J. Pathol. (1)

O. De Wever and M. Mareel, “Role of tissue stroma in cancer cell invasion,” J. Pathol. 200(4), 429–447 (2003).
[CrossRef] [PubMed]

Journal of Raman Spectroscopy (1)

H. Rokos, J. Moore, S. Hasse, J. M. Gillbro, J. M. Wood, and K. U. Schallreuter, “In vivo fluorescence excitation spectroscopy andin vivo Fourier-transform Raman spectroscopy in human skin: evidence of H2O2 oxidation of epidermal albumin in patients with vitiligo,” Journal of Raman Spectroscopy 35(2), 125–130 (2004).
[CrossRef]

Lasers Surg. Med. (1)

C. A. Lieber, S. K. Majumder, D. L. Ellis, D. D. Billheimer, and A. Mahadevan-Jansen, “In vivo nonmelanoma skin cancer diagnosis using Raman microspectroscopy,” Lasers Surg. Med. 40(7), 461–467 (2008).
[CrossRef] [PubMed]

Photochem. Photobiol. (1)

A. Robichaux Viehoever, D. Anderson, D. Jansen, and A. Mahadevan-Jansen, “Organotypic raft cultures as an effective in vitro tool for understanding Raman spectral analysis of tissue,” Photochem. Photobiol. 78(5), 517–524 (2003).
[CrossRef] [PubMed]

Other (1)

H. E. Nieburgs, R. G. Zak, D. C. Allen, H. Reisman, and T. Clardy, “Systemic cellular changes in material from human and animal tissues in presence of tumors,” Transactions of the Seventh Annual Meeting of the International Society of Cytology Council 137(1959).

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Figures (3)

Fig. 1
Fig. 1

Mean Raman spectra of the raft layers after co-culture with the adherent cell cultures for both the (A) epidermis and (B) dermis. Difference spectra (tumor-associated minus naïve; +/− standard deviation) are also shown to clarify Raman spectral changes in the (C) epidermis and (D) dermis.

Fig. 2
Fig. 2

Principal component analysis of the Raman spectral measurements of the two raft populations (red triangles = naïve, green asterisks = tumor-associated) shows overall separation in both (A) epidermis and (B) dermis using just two component scores for each stratum. Component loadings for (C) epidermis and (D) dermis reveal similar band positions to mean difference spectra.

Fig. 3
Fig. 3

(A) Mean Raman spectra of the cell lines used to induce changes in tissue rafts, and (B) their difference spectrum with the mean epidermal and dermal raft difference spectra.

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