Abstract

Owing to its tissue-penetration ability, multi-photon fluorescence microscopy allows for the high-resolution, non-invasive imaging of deep tissue in vivo; the recently developed three-photon microscopy (3PM) has extended the depth of high-resolution, non-invasive functional imaging of mouse brains to beyond 1.0 mm. However, the low repetition rate of femtosecond lasers that are normally used in 3PM limits the temporal resolution of point-scanning three-photon microscopy. To increase the volumetric imaging speed of 3PM, we propose a combination of an axially elongated needle-like Bessel-beam with three-photon excitation (3PE) to image biological samples with an extended depth of focus. We demonstrate the higher signal-to-background ratio (SBR) of the Bessel-beam 3PM compared to the two-photon version both theoretically and experimentally. Finally, we perform simultaneous calcium imaging of brain regions at different axial locations in live fruit flies and rapid volumetric imaging of neuronal structures in live mouse brains. These results highlight the unique advantage of conducting rapid volumetric imaging with a high SBR in the deep brain in vivo using scanning Bessel-3PM.

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Full Article  |  PDF Article
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References

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  1. W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
    [Crossref]
  2. W. Denk and F. Helmchen, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
    [Crossref]
  3. W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
    [Crossref]
  4. D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express 17(16), 13354–13364 (2009).
    [Crossref]
  5. N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
    [Crossref]
  6. D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
    [Crossref]
  7. L. Cheng, N. G. Horton, K. Wang, S. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
    [Crossref]
  8. E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
    [Crossref]
  9. W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
    [Crossref]
  10. G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
    [Crossref]
  11. E. J. Botcherby, R. Juskaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field,” Opt. Commun. 268(2), 253–260 (2006).
    [Crossref]
  12. P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).
  13. G. Theriault, Y. D. Koninck, and N. McCarthy, “Extended depth of field microscopy for rapid volumetric two-photon imaging,” Opt. Express 21(8), 10095–10104 (2013).
    [Crossref]
  14. R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
    [Crossref]
  15. B. Chen, T. Jiang, W. Zong, L. Chen, Z. Zhang, and A. Wang, “910nm femtosecond Nd-doped fiber laser for in vivo two-photon microscopic imaging,” Opt. Express 24(15), 16544–16549 (2016).
    [Crossref]
  16. B. Chen, H. Rong, X. Huang, R. Wu, D. Wu, Y. Li, L. Feng, Z. Zhang, L. Chen, and A. Wang, “Robust hollow-fiber-pigtailed 930 nm femtosecond Nd:fiber laser for volumetric two-photon imaging,” Opt. Express 25(19), 22704–22709 (2017).
    [Crossref]
  17. L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
    [Crossref]
  18. M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
    [Crossref]
  19. C. Xu and W. W. Webb, “Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy,” in Topics in Fluorescence Spectroscopy, J. R. Lakowiczv, ed. (Kluwer Academic Publishers, 1997).
  20. O. Brzobohaty, T. Cizmar, and P. Zemanek, “High quality quasi-Bessel beam generated by round-tip axicon,” Opt. Express 16(17), 12688–12700 (2008).
    [Crossref]
  21. R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
    [Crossref]
  22. J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
    [Crossref]
  23. J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
    [Crossref]
  24. M. Heisenberg, “Mushroom Body Memoir: From Maps to Models,” Nature 4(4), 266–275 (2003).

2017 (3)

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

B. Chen, H. Rong, X. Huang, R. Wu, D. Wu, Y. Li, L. Feng, Z. Zhang, L. Chen, and A. Wang, “Robust hollow-fiber-pigtailed 930 nm femtosecond Nd:fiber laser for volumetric two-photon imaging,” Opt. Express 25(19), 22704–22709 (2017).
[Crossref]

2016 (2)

B. Chen, T. Jiang, W. Zong, L. Chen, Z. Zhang, and A. Wang, “910nm femtosecond Nd-doped fiber laser for in vivo two-photon microscopic imaging,” Opt. Express 24(15), 16544–16549 (2016).
[Crossref]

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

2014 (1)

2013 (3)

G. Theriault, Y. D. Koninck, and N. McCarthy, “Extended depth of field microscopy for rapid volumetric two-photon imaging,” Opt. Express 21(8), 10095–10104 (2013).
[Crossref]

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

2009 (1)

2008 (3)

O. Brzobohaty, T. Cizmar, and P. Zemanek, “High quality quasi-Bessel beam generated by round-tip axicon,” Opt. Express 16(17), 12688–12700 (2008).
[Crossref]

G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref]

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
[Crossref]

2006 (2)

E. J. Botcherby, R. Juskaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field,” Opt. Commun. 268(2), 253–260 (2006).
[Crossref]

P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).

2005 (1)

W. Denk and F. Helmchen, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref]

2003 (3)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref]

M. Heisenberg, “Mushroom Body Memoir: From Maps to Models,” Nature 4(4), 266–275 (2003).

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

2002 (1)

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

1997 (1)

R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
[Crossref]

1996 (1)

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

1990 (1)

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref]

Armstrong, J. D.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Axel, R.

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

Belle, J. S.

R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
[Crossref]

Bierfeld, J.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Booth, M. J.

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
[Crossref]

Botcherby, E. J.

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
[Crossref]

E. J. Botcherby, R. Juskaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field,” Opt. Commun. 268(2), 253–260 (2006).
[Crossref]

Brzobohaty, O.

Chen, B.

Chen, L.

Chen, S.

Cheng, L.

Cheng, Y.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Cizmar, T.

Clark, C. G.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

Connolly, J. B.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Deisseroth, K.

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

Denk, W.

W. Denk and F. Helmchen, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref]

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref]

Dufour, P.

P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).

Durst, M. E.

Feng, D. D.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Feng, L.

Fink, R.

G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref]

Fitzpatrick, D.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Flores, J.

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

Forte, M.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Gendre, N.

R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
[Crossref]

Heimbeck, G.

R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
[Crossref]

Heisenberg, M.

M. Heisenberg, “Mushroom Body Memoir: From Maps to Models,” Nature 4(4), 266–275 (2003).

Helmchen, F.

W. Denk and F. Helmchen, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref]

Hernandez, J. C. C.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Horton, N. G.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

L. Cheng, N. G. Horton, K. Wang, S. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
[Crossref]

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

Huang, X.

Ji, N.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Jiang, T.

Juskaitis, R.

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
[Crossref]

E. J. Botcherby, R. Juskaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field,” Opt. Commun. 268(2), 253–260 (2006).
[Crossref]

Kaiser, K.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Kelleher, K.

G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref]

Kerlin, A.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Kobat, D.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express 17(16), 13354–13364 (2009).
[Crossref]

Koninck, Y. D.

G. Theriault, Y. D. Koninck, and N. McCarthy, “Extended depth of field microscopy for rapid volumetric two-photon imaging,” Opt. Express 21(8), 10095–10104 (2013).
[Crossref]

P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).

Koyama, M.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Li, Y.

B. Chen, H. Rong, X. Huang, R. Wu, D. Wu, Y. Li, L. Feng, Z. Zhang, L. Chen, and A. Wang, “Robust hollow-fiber-pigtailed 930 nm femtosecond Nd:fiber laser for volumetric two-photon imaging,” Opt. Express 25(19), 22704–22709 (2017).
[Crossref]

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

Liang, L.

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

Liang, Y.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Lima, S. Q.

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

Lu, R.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Luo, L.

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

McCarthy, N.

G. Theriault, Y. D. Koninck, and N. McCarthy, “Extended depth of field microscopy for rapid volumetric two-photon imaging,” Opt. Express 21(8), 10095–10104 (2013).
[Crossref]

P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).

Miesenboeck, G.

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

Miller, J. K.

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Mohar, B.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Morcillo, P.

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

Ng, M.

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

Nishimura, N.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express 17(16), 13354–13364 (2009).
[Crossref]

O’Kane, C. J.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Orger, M. B.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Ouzounov, D. G.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Paninski, R.

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Peterka, D. S.

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Piché, M.

P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).

Pnevmatikakis, L.

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Potter, C. J.

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

Reddy, G. D.

G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref]

Reid, L. C.

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Reimer, J.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Roberts, I. J. H.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Rong, H.

Roorda, R. D.

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

Saggau, P.

G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref]

Schaffer, C. B.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express 17(16), 13354–13364 (2009).
[Crossref]

Scholl, B.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Seelig, J. D.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Stocker, R. F.

R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
[Crossref]

Strickler, J. H.

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref]

Sun, W.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Tanimoto, M.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Theriault, G.

Tolias, A. S.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Tsien, R. W.

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

Tully, T.

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Vosshall, L. B.

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

Wang, A.

Wang, J. W.

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

Wang, K.

L. Cheng, N. G. Horton, K. Wang, S. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
[Crossref]

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

Wang, M.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Wang, T.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Webb, W. W.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref]

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref]

Williams, R. M.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref]

Wilson, D. E.

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

Wilson, T.

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
[Crossref]

E. J. Botcherby, R. Juskaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field,” Opt. Commun. 268(2), 253–260 (2006).
[Crossref]

Wise, F. W.

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

Wong, A. M.

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

Wong, A. W.

Wu, D.

Wu, R.

Xu, C.

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

L. Cheng, N. G. Horton, K. Wang, S. Chen, and C. Xu, “Measurements of multiphoton action cross sections for multiphoton microscopy,” Biomed. Opt. Express 5(10), 3427–3433 (2014).
[Crossref]

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

D. Kobat, M. E. Durst, N. Nishimura, A. W. Wong, C. B. Schaffer, and C. Xu, “Deep tissue multiphoton microscopy using longer wavelength excitation,” Opt. Express 17(16), 13354–13364 (2009).
[Crossref]

Yang, W.

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Yizhar, O.

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

Yuste,

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Zemanek, P.

Zemelman, B. V.

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

Zhang, Z.

Zipfel, W. R.

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref]

Zong, W.

Appl. Opt. (1)

P. Dufour, M. Piché, Y. D. Koninck, and N. McCarthy, “Two-photon excitation fluorescence microscopy with a high depth of field using an axicon,” Appl. Opt. 45(36), 9245–9252 (2006).

Biomed. Opt. Express (1)

Cell (1)

J. W. Wang, A. M. Wong, J. Flores, L. B. Vosshall, and R. Axel, “Two-Photon Calcium Imaging Reveals an Odor-Evoked Map of Activity in the Fly Brain,” Cell 112(2), 271–282 (2003).
[Crossref]

J. Neurobiol. (1)

R. F. Stocker, G. Heimbeck, N. Gendre, and J. S. Belle, “Neuroblast Ablation in Drosophila P[GAL4] Lines Reveals Origins of Olfactory Interneurons,” J. Neurobiol. 32(5), 443–456 (1997).
[Crossref]

Nat. Biotechnol. (1)

W. R. Zipfel, R. M. Williams, and W. W. Webb, “Nonlinear magic: multiphoton microscopy in the biosciences,” Nat. Biotechnol. 21(11), 1369–1377 (2003).
[Crossref]

Nat. Methods (2)

W. Denk and F. Helmchen, “Deep tissue two-photon microscopy,” Nat. Methods 2(12), 932–940 (2005).
[Crossref]

D. G. Ouzounov, T. Wang, M. Wang, D. D. Feng, N. G. Horton, J. C. C. Hernandez, Y. Cheng, J. Reimer, A. S. Tolias, N. Nishimura, and C. Xu, “In vivo three-photon imaging of activity of GCaMP6-labeled neurons deep in intact mouse brain,” Nat. Methods 14(4), 388–390 (2017).
[Crossref]

Nat. Neurosci. (2)

R. Lu, W. Sun, Y. Liang, A. Kerlin, J. Bierfeld, J. D. Seelig, D. E. Wilson, B. Scholl, B. Mohar, M. Tanimoto, M. Koyama, D. Fitzpatrick, M. B. Orger, and N. Ji, “Video-rate volumetric functional imaging of the brain at synaptic resolution,” Nat. Neurosci. 20(4), 620–628 (2017).
[Crossref]

G. D. Reddy, K. Kelleher, R. Fink, and P. Saggau, “Three-dimensional random access multiphoton microscopy for fast functional imaging of neuronal activity,” Nat. Neurosci. 11(6), 713–720 (2008).
[Crossref]

Nat. Photonics (1)

N. G. Horton, K. Wang, D. Kobat, C. G. Clark, F. W. Wise, C. B. Schaffer, and C. Xu, “In vivo three-photon microscopy of subcortical structure within an intact mouse brain,” Nat. Photonics 7(3), 205–209 (2013).
[Crossref]

Nature (1)

M. Heisenberg, “Mushroom Body Memoir: From Maps to Models,” Nature 4(4), 266–275 (2003).

Neuron (3)

L. Liang, Y. Li, C. J. Potter, O. Yizhar, K. Deisseroth, R. W. Tsien, and L. Luo, “GABAergic projection neurons route selective olfactory inputs to specific higher-order neurons,” Neuron 79(5), 917–931 (2013).
[Crossref]

M. Ng, R. D. Roorda, S. Q. Lima, B. V. Zemelman, P. Morcillo, and G. Miesenboeck, “Transmission of olfactory information between three populations of neurons in the antennal lobe of the fly,” Neuron 36(3), 463–474 (2002).
[Crossref]

W. Yang, J. K. Miller, and L. C. Reid, EL. Pnevmatikakis, R. Paninski, Yuste, and D. S. Peterka, “Simultaneous multi-plane imaging of neural circuits,” Neuron 89(2), 269–284 (2016).
[Crossref]

Opt. Commun. (2)

E. J. Botcherby, R. Juskaitis, and T. Wilson, “Scanning two photon fluorescence microscopy with extended depth of field,” Opt. Commun. 268(2), 253–260 (2006).
[Crossref]

E. J. Botcherby, R. Juskaitis, M. J. Booth, and T. Wilson, “An optical technique for remote focusing in microscopy,” Opt. Commun. 281(4), 880–887 (2008).
[Crossref]

Opt. Express (5)

Science (2)

W. Denk, J. H. Strickler, and W. W. Webb, “Two-photon laser scanning fluorescence microscopy,” Science 248(4951), 73–76 (1990).
[Crossref]

J. B. Connolly, I. J. H. Roberts, J. D. Armstrong, K. Kaiser, M. Forte, T. Tully, and C. J. O’Kane, “Associative Learning Disrupted by Impaired Gs Signaling in Drosophila Mushroom Bodies,” Science 274(5295), 2104–2107 (1996).
[Crossref]

Other (1)

C. Xu and W. W. Webb, “Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy,” in Topics in Fluorescence Spectroscopy, J. R. Lakowiczv, ed. (Kluwer Academic Publishers, 1997).

Cited By

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Figures (4)

Fig. 1
Fig. 1 Schematic diagram of the apparatus. Flipper mirrors: F1 was used to switch between 920 nm and 1300 nm lasers; F2 and F3 were used to switch between the Gaussian and Bessel beam excitation. Axicon, α = 2 degrees. Lenses: L1 and L2, 50 mm (or 75, 100 mm) focal length, determines the effective excitation NAs and lengths of the Bessel beam. L3 (100 mm), L4 (100 mm), SL (54 mm) and TL (200 mm) were used to create multiple conjugate planes of the back focal plane (BFP) of the objective in the light path; L5, collection lens (30 mm). Galvo mirrors: GMX/GMY, for the x/y direction of beam scanning. DM, 735-nm long-pass dichroic mirror; a 770-nm short pass filter and a 525/40 nm bandpass filter were used here (Filters) for emission collection.
Fig. 2
Fig. 2 Theoretically simulated and experimentally determined PSFs. (a)(b), simulated PSFs of 3PM and 2PM along the x-z planes. (c)(d), the experimental results. Scale bars, 100 μm (z), 2 μm (x). 0.5-μm fluorescent beads were used to measure xyz profiles of Bessel-3PM and Bessel-2PM. (e)(g), x-y cross section after integration of the z-axis of the Bessel beam to numerically simulate the fluorescence distribution of the side lobes (e, 3PM; g, 2PM), (f)(h), the experimental results. Scale bar, 2 µm. (i-l), comparison of blood vessels in live zebrafish imaged with Bessel-3PM and 2PM. (i), a representative example of the fish under Bessel 2PM and 3PM, in which the boxed region was magnified in j and k. (l), fluorescence intensities along the line across the same blood vessels.
Fig. 3
Fig. 3 Projection of volumetric imaging of GCaMP-labeled neuronal Ca2+ transients in olfactory centers of Drosophila in vivo. We stimulated the fly with isoamyl acetate (IA) for 5 s to trigger neuronal activities at the LH and the AL (DM2 glomerulus) of ePNs. (a), 3D neuronal structures under the point-scanning 3PM with color-coded by depth. (b), projection of 3D volume of neuronal structure under the Bessel-3PM. (c)(d), pseudo-color snapshots of the Ca2+ signals upon mineral oil and IA. (e), time-dependent Ca2+ responses from regions at the LH and the DM2 were recorded at a scanning rate of 5 Hz in experiments (c) and (d). Blue bar, stimulation duration. Individual traces are averaged results of 2-4 repeats and are processed with 2 × binning. Error bars, ± s.e.m. (f-j), similar experiments as (a)-(e), except that Kenyon cells at the MB were imaged. Axial FWHM of the Bessel beam, 110 μm. NA, 0.6.
Fig. 4
Fig. 4 In vivo three-photon images of neural cortex in a Thy1-YFP transgenic mouse. (a), projection of 3D volume neurons and neurites in cortex of the awake mouse taken with Bessel-beam at a frame rate of 1 Hz (image averaged from 10 frames afterwards). (b), mean intensity projection of a 65-μm-thick image stack collected at 1-μm z steps in point-scanning mode, from the same region as in (a). The stack covers from 620 μm to 685 μm below the dura, with structures color-coded by depth. The image of each layer was averaged from 3 frames, with a post objective power of 10 mW for Gaussian beam scanning and 110 mW for Bessel beam scanning.

Equations (6)

Equations on this page are rendered with MathJax. Learn more.

F ( t ) n = C n I 0 n (t) dr S n (r)
S( r,z )= S 1 (z) S 2 (r)
S 1 ( z )= 4 π 2 NA 2 z λ exp( 2 NA 2 z 2 w 0 2 )
S 2 ( r )= J 0 2 ( 2πrNA λ )
F ( t ) nm = C n I 0 n (t) S 1 (z) n dz 0 2π dθ λ 2πNA x m1 0 λ 2πNA x m 0 r S 2 (r) n dr
F ( t ) nm F ( t ) n1 = x m1 0 x m 0 r ' J 0 2n (r')dr' 0 x 1 0 r ' J 0 2n (r')dr' .