Abstract

The spatial organization of stromal collagen of ex-vivo corneas has been quantified in adaptive-optics second harmonic generation (SHG) images by means of an optimized Fourier transform (FT) based analysis. At a particular depth location, adjacent lamellae often present similar orientations and run parallel to the corneal surface. However this pattern might be combined with interweaved collagen bundles leading to crosshatched structures with different orientations. The procedure here reported provides us with both principal and crosshatched angles. This is also able to automatically distinguish a random distribution from a cross-shaped one, since it uses the ratio of the axes lengths of the best-fitted ellipse of the FT data as an auxiliary parameter. The technique has successfully been applied to SHG images of healthy corneas (both stroma and Bowman’s layer) of different species and to corneas undergoing cross-linking treatment.

© 2013 OSA

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    [CrossRef] [PubMed]
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    [CrossRef] [PubMed]
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    [CrossRef]

2012

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Y. Mega, M. Robitaille, R. Zareian, J. McLean, J. Ruberti, and C. DiMarzio, “Quantification of lamellar orientation in corneal collagen using second harmonic generation images,” Opt. Lett.37(16), 3312–3314 (2012).
[CrossRef] [PubMed]

R. Ambekar, T.-Y. Lau, M. Walsh, R. Bhargava, and K. C. Toussaint., “Quantifying collagen structure in breast biopsies using second-harmonic generation imaging,” Biomed. Opt. Express3(9), 2021–2035 (2012).
[CrossRef] [PubMed]

2011

Q. Wu, B. E. Applegate, and A. T. Yeh, “Cornea microstructure and mechanical responses measured with nonlinear optical and optical coherence microscopy using sub-10-fs pulses,” Biomed. Opt. Express2(5), 1135–1146 (2011).
[CrossRef] [PubMed]

E. J. Gualda, J. R. Vázquez de Aldana, M. C. Martínez-García, P. Moreno, J. Hernández-Toro, L. Roso, P. Artal, and J. M. Bueno, “Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas,” Biomed. Opt. Express2(11), 2950–2960 (2011).
[CrossRef] [PubMed]

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, and P. Artal, “Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy,” Cornea30(6), 692–701 (2011).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

2010

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, and P. Artal, “Adaptive optics multiphoton microscopy to study ex vivo ocular tissues,” J. Biomed. Opt.15(6), 066004 (2010).
[CrossRef] [PubMed]

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

M. Sivaguru, S. Durgam, R. Ambekar, D. Luedtke, G. Fried, A. Stewart, and K. C. Toussaint., “Quantitative analysis of collagen fiber organization in injured tendons using Fourier transform-second harmonic generation imaging,” Opt. Express18(24), 24983–24993 (2010).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, and P. Artal, “Non-linear 3D microscopy of ex vivo corneas,” Proc. SPIE7569, 756937, 756937-4 (2010).
[CrossRef]

2009

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

P. Matteini, F. Ratto, F. Rossi, R. Cicchi, C. Stringari, D. Kapsokalyvas, F. S. Pavone, and R. Pini, “Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging,” Opt. Express17(6), 4868–4878 (2009).
[CrossRef] [PubMed]

R. A. R. Rao, M. R. Mehta, and K. C. Toussaint., “Fourier transform-second-harmonic generation imaging of biological tissues,” Opt. Express17(17), 14534–14542 (2009).
[CrossRef] [PubMed]

R. A. R. Rao, M. R. Mehta, S. Leithem, and K. C. Toussaint., “Quantitative analysis of forward and backward second-harmonic images of collagen fibers using Fourier transform second-harmonic-generation microscopy,” Opt. Lett.34(24), 3779–3781 (2009).
[CrossRef] [PubMed]

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

2007

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

F. Rossi, P. Matteini, I. Bruno, P. Nesi, and R. Pini, “Monitoring thermally-induced phase transitions in porcine cornea with the use of fluorescence micro-imaging analysis,” Opt. Express15(18), 11178–11184 (2007).
[CrossRef] [PubMed]

2006

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

G. Wollensak, “Crosslinking treatment of progressive keratoconus: new hope,” Curr. Opin. Ophthalmol.17(4), 356–360 (2006).
[CrossRef] [PubMed]

2005

2002

1991

Y. Komai and T. Ushiki, “The three-dimensional organization of collagen fibrils in the human cornea and sclera,” Invest. Ophthalmol. Vis. Sci.32(8), 2244–2258 (1991).
[PubMed]

Ambekar, R.

Applegate, B. E.

Aptel, F.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Arnold, C. L.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Artal, P.

J. M. Bueno, E. J. Gualda, and P. Artal, “Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy,” Cornea30(6), 692–701 (2011).
[CrossRef] [PubMed]

E. J. Gualda, J. R. Vázquez de Aldana, M. C. Martínez-García, P. Moreno, J. Hernández-Toro, L. Roso, P. Artal, and J. M. Bueno, “Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas,” Biomed. Opt. Express2(11), 2950–2960 (2011).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, and P. Artal, “Non-linear 3D microscopy of ex vivo corneas,” Proc. SPIE7569, 756937, 756937-4 (2010).
[CrossRef]

J. M. Bueno, E. J. Gualda, and P. Artal, “Adaptive optics multiphoton microscopy to study ex vivo ocular tissues,” J. Biomed. Opt.15(6), 066004 (2010).
[CrossRef] [PubMed]

Basta-Pljakic, J.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Beaurepaire, E.

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Bhargava, R.

Bille, J.

Boote, C.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Brown, D. J.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Bruno, I.

Bueno, J. M.

J. M. Bueno, E. J. Gualda, and P. Artal, “Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy,” Cornea30(6), 692–701 (2011).
[CrossRef] [PubMed]

E. J. Gualda, J. R. Vázquez de Aldana, M. C. Martínez-García, P. Moreno, J. Hernández-Toro, L. Roso, P. Artal, and J. M. Bueno, “Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas,” Biomed. Opt. Express2(11), 2950–2960 (2011).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, and P. Artal, “Non-linear 3D microscopy of ex vivo corneas,” Proc. SPIE7569, 756937, 756937-4 (2010).
[CrossRef]

J. M. Bueno, E. J. Gualda, and P. Artal, “Adaptive optics multiphoton microscopy to study ex vivo ocular tissues,” J. Biomed. Opt.15(6), 066004 (2010).
[CrossRef] [PubMed]

Chai, D.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

Chang, Y.-L.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

Chen, J.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Chen, S. J.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

Chen, S.-J.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

Chen, W. L.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

Chen, W.-L.

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Chen, Y. F.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Chikama, T.

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Cicchi, R.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

P. Matteini, F. Ratto, F. Rossi, R. Cicchi, C. Stringari, D. Kapsokalyvas, F. S. Pavone, and R. Pini, “Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging,” Opt. Express17(6), 4868–4878 (2009).
[CrossRef] [PubMed]

Coudrillier, B.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Courjaud, A.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Crotti, C.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

De Giorgi, V.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

Deloison, F.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Deniset-Besseau, A.

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

DiMarzio, C.

Dong, C. Y.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Dong, C.-Y.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Druon, F.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Durgam, S.

Flatow, E. L.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Fried, G.

Fung, D. T.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Georges, P.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Ghazaryan, A. A.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

Giakoumaki, A.

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

Giese, G.

Gualda, E. J.

E. J. Gualda, J. R. Vázquez de Aldana, M. C. Martínez-García, P. Moreno, J. Hernández-Toro, L. Roso, P. Artal, and J. M. Bueno, “Femtosecond infrared intrastromal ablation and backscattering-mode adaptive-optics multiphoton microscopy in chicken corneas,” Biomed. Opt. Express2(11), 2950–2960 (2011).
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J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
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J. M. Bueno, E. J. Gualda, and P. Artal, “Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy,” Cornea30(6), 692–701 (2011).
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J. M. Bueno, E. J. Gualda, and P. Artal, “Adaptive optics multiphoton microscopy to study ex vivo ocular tissues,” J. Biomed. Opt.15(6), 066004 (2010).
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J. M. Bueno, E. J. Gualda, and P. Artal, “Non-linear 3D microscopy of ex vivo corneas,” Proc. SPIE7569, 756937, 756937-4 (2010).
[CrossRef]

Guthoff, R. F.

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

Han, M.

Hanna, M.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Hernández-Toro, J.

Hovakimyan, M.

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

Hovhannisyan, V.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

Hovhannisyan, V. A.

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Hseuh, C.-M.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

Hsiao, C. H.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Hsueh, C. M.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

Hsueh, C.-M.

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Huang, S. C.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Huq, R.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Hüttmann, G.

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

Jee, S. H.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Jepsen, K. J.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Jester, B.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

Jester, J. V.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Jiang, X.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Juhasz, T.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

Kapsokalyvas, D.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

P. Matteini, F. Ratto, F. Rossi, R. Cicchi, C. Stringari, D. Kapsokalyvas, F. S. Pavone, and R. Pini, “Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging,” Opt. Express17(6), 4868–4878 (2009).
[CrossRef] [PubMed]

Kawamoto, K.

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Kenney, M. C.

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Komai, Y.

Y. Komai and T. Ushiki, “The three-dimensional organization of collagen fibrils in the human cornea and sclera,” Invest. Ophthalmol. Vis. Sci.32(8), 2244–2258 (1991).
[PubMed]

Kriling, S.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

Lau, T.-Y.

Laudier, D. M.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Legeais, J.-M.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Leithem, S.

Lin, S. J.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Lin, W. C.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Liu, G.-Y.

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Liu, J.-S.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

Lo, W.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Lotti, T.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

Lu, K.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Luedtke, D.

Ma, D. H. K.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

Maio, V.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

Marcos, S.

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

Martínez-García, M. C.

Massi, D.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

Matteini, P.

McLean, J.

Meek, K. M.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Mega, Y.

Mehta, M. R.

Moreno, P.

Morin, F.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Morishige, N.

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Mottay, É.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Nassif, N.

Nesi, P.

Nguyen, T. D.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Nien, C. J.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

Nishida, T.

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Nuzzo, V.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Olivier, N.

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Pavone, F. S.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

P. Matteini, F. Ratto, F. Rossi, R. Cicchi, C. Stringari, D. Kapsokalyvas, F. S. Pavone, and R. Pini, “Photothermally-induced disordered patterns of corneal collagen revealed by SHG imaging,” Opt. Express17(6), 4868–4878 (2009).
[CrossRef] [PubMed]

Pérez-Merino, P.

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

Peyrot, D. A.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Pijanka, J. K.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Pini, R.

Plamann, K.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Quigley, H. A.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Rao, R. A. R.

Ratto, F.

Robitaille, M.

Roso, L.

Rossi, F.

Ruberti, J.

Savoldelli, M.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Schaffler, M. B.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Schanne-Klein, M.-C.

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Sereysky, J. B.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Sivaguru, M.

Sorensen, T.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Stachs, O.

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

Steven, P.

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

Stewart, A.

Stringari, C.

Sun, Y.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Takagi, Y.

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

Takahara, A.

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

Tan, H. Y.

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Tan, H.-Y.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Toussaint, K. C.

Tromberg, B. J.

Ushiki, T.

Y. Komai and T. Ushiki, “The three-dimensional organization of collagen fibrils in the human cornea and sclera,” Invest. Ophthalmol. Vis. Sci.32(8), 2244–2258 (1991).
[PubMed]

Van Wiechen, A.

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

Vázquez de Aldana, J. R.

Wahlert, A. J.

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

Walsh, M.

Wang, S.-S.

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Winkler, M.

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

Wollensak, G.

G. Wollensak, “Crosslinking treatment of progressive keratoconus: new hope,” Curr. Opin. Ophthalmol.17(4), 356–360 (2006).
[CrossRef] [PubMed]

Wu, Q.

Yeh, A. T.

Yu, H. S.

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

Zareian, R.

Zhao, J.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Zheng, L.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Zhu, X.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Zhuo, S.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

Ziegler, K.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

Zoumi, A.

Ann. Biomed. Eng.

D. T. Fung, J. B. Sereysky, J. Basta-Pljakic, D. M. Laudier, R. Huq, K. J. Jepsen, M. B. Schaffler, and E. L. Flatow, “Second harmonic generation imaging and Fourier transform spectral analysis reveal damage in fatigue-loaded tendons,” Ann. Biomed. Eng.38(5), 1741–1751 (2010).
[CrossRef] [PubMed]

Biomed. Opt. Express

Biophys. J.

C.-M. Hsueh, W. Lo, W.-L. Chen, V. A. Hovhannisyan, G.-Y. Liu, S.-S. Wang, H.-Y. Tan, and C.-Y. Dong, “Structural characterization of edematous corneas by forward and backward second harmonic generation imaging,” Biophys. J.97(4), 1198–1205 (2009).
[CrossRef] [PubMed]

Cornea

J. M. Bueno, E. J. Gualda, and P. Artal, “Analysis of corneal stroma organization with wavefront optimized nonlinear microscopy,” Cornea30(6), 692–701 (2011).
[CrossRef] [PubMed]

Curr. Opin. Ophthalmol.

G. Wollensak, “Crosslinking treatment of progressive keratoconus: new hope,” Curr. Opin. Ophthalmol.17(4), 356–360 (2006).
[CrossRef] [PubMed]

Invest. Ophthalmol. Vis. Sci.

J. K. Pijanka, B. Coudrillier, K. Ziegler, T. Sorensen, K. M. Meek, T. D. Nguyen, H. A. Quigley, and C. Boote, “Quantitative mapping of collagen fiber orientation in non-glaucoma and glaucoma posterior human sclerae,” Invest. Ophthalmol. Vis. Sci.53(9), 5258–5270 (2012).
[CrossRef] [PubMed]

W. Lo, W. L. Chen, C. M. Hsueh, A. A. Ghazaryan, S. J. Chen, D. H. K. Ma, C. Y. Dong, and H. Y. Tan, “Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea,” Invest. Ophthalmol. Vis. Sci.53(7), 3501–3507 (2012).
[CrossRef] [PubMed]

N. Morishige, Y. Takagi, T. Chikama, A. Takahara, and T. Nishida, “Three-dimensional analysis of collagen lamellae in the anterior stroma of the human cornea visualized by second harmonic generation imaging microscopy,” Invest. Ophthalmol. Vis. Sci.52(2), 911–915 (2011).
[CrossRef] [PubMed]

M. Winkler, D. Chai, S. Kriling, C. J. Nien, D. J. Brown, B. Jester, T. Juhasz, and J. V. Jester, “Nonlinear optical macroscopic assessment of 3-D corneal collagen organization and axial biomechanics,” Invest. Ophthalmol. Vis. Sci.52(12), 8818–8827 (2011).
[CrossRef] [PubMed]

H. Y. Tan, Y. Sun, W. Lo, S. J. Lin, C. H. Hsiao, Y. F. Chen, S. C. Huang, W. C. Lin, S. H. Jee, H. S. Yu, and C. Y. Dong, “Multiphoton fluorescence and second harmonic generation imaging of the structural alterations in keratoconus ex vivo,” Invest. Ophthalmol. Vis. Sci.47(12), 5251–5259 (2006).
[CrossRef] [PubMed]

N. Morishige, A. J. Wahlert, M. C. Kenney, D. J. Brown, K. Kawamoto, T. Chikama, T. Nishida, and J. V. Jester, “Second-harmonic imaging microscopy of normal human and keratoconus cornea,” Invest. Ophthalmol. Vis. Sci.48(3), 1087–1094 (2007).
[CrossRef] [PubMed]

F. Aptel, N. Olivier, A. Deniset-Besseau, J.-M. Legeais, K. Plamann, M.-C. Schanne-Klein, and E. Beaurepaire, “Multimodal nonlinear imaging of the human cornea,” Invest. Ophthalmol. Vis. Sci.51(5), 2459–2465 (2010).
[CrossRef] [PubMed]

Y. Komai and T. Ushiki, “The three-dimensional organization of collagen fibrils in the human cornea and sclera,” Invest. Ophthalmol. Vis. Sci.32(8), 2244–2258 (1991).
[PubMed]

J. M. Bueno, E. J. Gualda, A. Giakoumaki, P. Pérez-Merino, S. Marcos, and P. Artal, “Multiphoton microscopy of ex vivo corneas after collagen cross-linking,” Invest. Ophthalmol. Vis. Sci.52(8), 5325–5331 (2011).
[CrossRef] [PubMed]

J. Biomed. Opt.

W. Lo, Y.-L. Chang, J.-S. Liu, C.-M. Hseuh, V. Hovhannisyan, S.-J. Chen, H.-Y. Tan, and C.-Y. Dong, “Multimodal, multiphoton microscopy and image correlation analysis for characterizing corneal thermal damage,” J. Biomed. Opt.14(5), 054003 (2009).
[CrossRef] [PubMed]

J. M. Bueno, E. J. Gualda, and P. Artal, “Adaptive optics multiphoton microscopy to study ex vivo ocular tissues,” J. Biomed. Opt.15(6), 066004 (2010).
[CrossRef] [PubMed]

J. Biophotonics

R. Cicchi, D. Kapsokalyvas, V. De Giorgi, V. Maio, A. Van Wiechen, D. Massi, T. Lotti, and F. S. Pavone, “Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy,” J. Biophotonics3(1-2), 34–43 (2010).
[CrossRef] [PubMed]

J. Cataract Refract. Surg.

P. Steven, M. Hovakimyan, R. F. Guthoff, G. Hüttmann, and O. Stachs, “Imaging corneal crosslinking by autofluorescence 2-photon microscopy, second harmonic generation, and fluorescence lifetime measurements,” J. Cataract Refract. Surg.36(12), 2150–2159 (2010).
[CrossRef] [PubMed]

J. Opt.

K. Plamann, F. Aptel, C. L. Arnold, A. Courjaud, C. Crotti, F. Deloison, F. Druon, P. Georges, M. Hanna, J.-M. Legeais, F. Morin, É. Mottay, V. Nuzzo, D. A. Peyrot, and M. Savoldelli, “Ultrashort pulse laser surgery of the cornea and the sclera,” J. Opt.12(8), 084002 (2010).
[CrossRef]

Opt. Express

Opt. Lett.

Proc. SPIE

J. M. Bueno, E. J. Gualda, and P. Artal, “Non-linear 3D microscopy of ex vivo corneas,” Proc. SPIE7569, 756937, 756937-4 (2010).
[CrossRef]

Skin Res. Technol.

K. Lu, J. Chen, S. Zhuo, L. Zheng, X. Jiang, X. Zhu, and J. Zhao, “Multiphoton laser scanning microscopy of localized scleroderma,” Skin Res. Technol.15(4), 489–495 (2009).
[CrossRef] [PubMed]

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