Abstract

In this experimental research, a label-free sensing strategy is developed and employed to detect adenosine triphosphate with utilization of aptamers, including exonuclease I and SYBR Gold. The conformation of aptamers bonding to the specific target molecule (ATP) is transformed into an antiparallel G-quadruplex structure from a random coil. Afterwards, considering the unfolded aptamers are the preferred substrates for exonuclease I, the addition of exonuclease I is used so as to digest unfolded aptamers in the mixture in a selective manner. In the follow-up study, in order to strengthen the fluorescence intensity, SYBR Gold is applied as a fluorescent probe. The aptasensor presents the features of high selectivity against adenosine triphosphate and the low detecting limit of concentrations (39.2 nM). In order to verify the validation of experimental procedures and the practical application of the aptasensor, the detection of adenosine triphosphate for human serum samples is performed with satisfactory success. The recovery result with the range of 93.8%–108.1% is desirable and suggests that the designed approach is applicable. The outcomes of the cellular adenosine triphosphate assay manifest that the level of adenosine triphosphate concentrations in cell extracts can be monitored without the interference of other substances in the cells. Subject to its advantageous benefits (cost-effective, easiness, rapidity, and extraordinary selectivity), the designed approach has a promising implication for adenosine triphosphate detection in the research domain of bioanalytical science and biology.

© 2021 The Author(s)

Measurement of adenosine triphosphate content in single red blood cells using the firefly bioluminescent reaction

R. K. Kostuk, A. G. Muhs, F. H. Kirkpatrick, and C. W. Gabel
Appl. Opt. 18(10) 1527-1532 (1979)

Composite membrane of graphene oxide and gold nanoparticles functionalized S fiber taper aptasensor for highly sensitive bisphenol A detection

Wanmei Guo, Yongsen Yu, Chao Xin, and Guangyong Jin
Opt. Lett. 49(6) 1616-1619 (2024)

Label-free cocaine aptasensor based on a long-period fiber grating

Anna Celebanska, Yasser Chiniforooshan, Monika Janik, Predrag Mikulic, Balasubramanian Sellamuthu, Ryan Walsh, Jonathan Perreault, and Wojtek J. Bock
Opt. Lett. 44(10) 2482-2485 (2019)

Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

Amos Danielli, Ady Arie, Noga Porat, and Marcelo Ehrlich
Opt. Express 16(23) 19253-19259 (2008)

Highly sensitive detection of cytochrome c in the NSCLC serum using a hydrophobic paper based–gold nanourchin substrate

Yue Sun, Shengjie Ge, Jin Xue, Xinyu Zhou, Wenbo Lu, Guang Li, and Xiaowei Cao
Biomed. Opt. Express 11(12) 7062-7078 (2020)