Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 75,
  • Issue 10,
  • pp. 1296-1304
  • (2021)

Characterization of Drug Resistance in Chronic Myeloid Leukemia Cells Based on Laser Tweezers Raman Spectroscopy

Not Accessible

Your library or personal account may give you access

Abstract

Multidrug resistance is highly associated with poor prognosis of chronic myeloid leukemia. This work aims to explore whether the laser tweezers Raman spectroscopy (LTRS) could be practical in separating adriamycin-resistant chronic myeloid leukemia cells K562/adriamycin from its parental cells K562, and to explore the potential mechanisms. Detection of LTRS initially reflected the spectral differences caused by chemoresistance including bands assigned to carbohydrates, amino acid, protein, lipids, and nucleic acid. In addition, principal components analysis as well as the classification and regression trees algorithms showed that the specificity and sensitivity were above 90%. Moreover, the band data-based classification and regression tree model and receiver operating characteristic curve further determined some important bands and band intensity ratios to be reliable indexes in discriminating K562 chemoresistance status. Finally, we highlighted three metabolism pathways correlated with chemoresistance. This work demonstrates that the label-free LTRS analysis combined with multivariate statistical analyses have great potential to be a novel analytical strategy at the single-cell level for rapid evaluation of the chemoresistance status of K562 cells.

© 2021 The Author(s)

PDF Article
More Like This
Leukemia cells detection based on electroporation assisted surface-enhanced Raman scattering

Yun Yu, Juqiang Lin, Duo Lin, Shangyuan Feng, Weiwei Chen, Zufang Huang, Hao Huang, and Rong Chen
Biomed. Opt. Express 8(9) 4108-4121 (2017)

Study on the chemodrug-induced effect in nasopharyngeal carcinoma cells using laser tweezer Raman spectroscopy

Sufang Qiu, Miaomiao Li, Jun Liu, Xiaochuan Chen, Ting Lin, Yunchao Xu, Yang Chen, Youliang Weng, Yuhui Pan, Shangyuan Feng, Xiandong Lin, Lurong Zhang, and Duo Lin
Biomed. Opt. Express 11(4) 1819-1833 (2020)

Automatic classification of acute and chronic myeloid leukemic cells with wide-angle label-free static cytometry

Linyan Xie, Qiao Liu, Changshun Shao, and Xuantao Su
Opt. Express 25(23) 29365-29373 (2017)

Supplementary Material (1)

NameDescription
Supplement 1       sj-pdf-1-asp-10.1177_00037028211024581 - Supplemental material for Characterization of Drug Resistance in Chronic Myeloid Leukemia Cells Based on Laser Tweezers Raman Spectroscopy

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved