Abstract

Resistance to radiotherapy has been an impediment in the treatment of cancer, and the inability to detect it at an early stage further exacerbates the prognosis. We have assessed the feasibility of Raman spectroscopy as a rapid assay for predicting radiosensitivity of cancer cells in comparison to the conventional biological assays. Cell lines derived from breast adenocarcinoma (MCF7), gingivobuccal squamous cell carcinoma (ITOC-03), and human embryonic kidney (HEK293) were subjected to varying doses of ionizing radiation. Cell viability of irradiated cells was assessed at different time points using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and Raman spectroscopy, and colony-forming capability was evaluated by clonogenic assay. Radiosensitivity observed using MTT assay was limited by the finding of similar cell viability in all the three cell lines 24 h post-irradiation. However, cell survival assessed using clonogenic assay and principal component linear discriminant analysis (PC-LDA) classification of Raman spectra showed correlating patterns. Irradiated cells showed loss of nucleic acid features and enhancement of 750 cm⁻¹ peak probably attributing to resonance Raman band of cytochromes in all three cell lines. PC-LDA analysis affirmed MCF7 to be a radioresistant cell line as compared to ITOC-03 and HEK293 to be the most radiosensitive cell line. Raman spectroscopy is shown to be a rapid and alternative assay for identification of radiosensitivity as compared to the gold standard clonogenic assay.

© 2020 The Author(s)

Discrimination of radiosensitive and radioresistant murine lymphoma cells by Raman spectroscopy and SERS

Iris Aguilar-Hernández, Diana L. Cárdenas-Chavez, Tzarara López-Luke, Alejandra García-García, Marcela Herrera-Domínguez, Eduardo Pisano, and Nancy Ornelas-Soto
Biomed. Opt. Express 11(1) 388-405 (2020)

Study on the chemodrug-induced effect in nasopharyngeal carcinoma cells using laser tweezer Raman spectroscopy

Sufang Qiu, Miaomiao Li, Jun Liu, Xiaochuan Chen, Ting Lin, Yunchao Xu, Yang Chen, Youliang Weng, Yuhui Pan, Shangyuan Feng, Xiandong Lin, Lurong Zhang, and Duo Lin
Biomed. Opt. Express 11(4) 1819-1833 (2020)

Anti-cancer effect of bee venom on human MDA-MB-231 breast cancer cells using Raman spectroscopy

Gyeong Bok Jung, Jeong-Eun Huh, Hyo-Jung Lee, Dohyun Kim, Gi-Ja Lee, Hun-Kuk Park, and Jae-Dong Lee
Biomed. Opt. Express 9(11) 5703-5718 (2018)

Raman spectroscopy combined with multivariate analysis to study the biochemical mechanism of lung cancer microwave ablation

Dongliang Song, Fan Yu, Shilin Chen, Yishen Chen, Qingli He, Zhe Zhang, Jingyuan Zhang, and Shuang Wang
Biomed. Opt. Express 11(2) 1061-1072 (2020)

Identifying non-muscle-invasive and muscle-invasive bladder cancer based on blood serum surface-enhanced Raman spectroscopy

Shuo Chen, Shanshan Zhu, Xiaoyu Cui, Wenbin Xu, Chuize Kong, Zhe Zhang, and Wei Qian
Biomed. Opt. Express 10(7) 3533-3544 (2019)