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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 74,
  • Issue 2,
  • pp. 241-244
  • (2020)

High-Frequency Raman Analysis in Biological Tissues Using Dual-Wavelength Excitation Raman Spectroscopy

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Abstract

A dual-wavelength excitation Raman probe with laser inputs at 866 nm or 1064 nm is customized and integrated into a compact Raman spectrometer that is based on an InGaAs detector. Under 1064 nm illumination, the spectrometer detects fingerprint Raman signals below 2000 cm–1. While under 866 nm illumination, the spectral range is extended to cover high-frequency region (2400–4000 cm–1) that includes major C–H and O–H Raman vibrations. We demonstrate that the dual excitation InGaAs Raman is beneficial in detecting high-frequency Raman signals, especially water contents in high-fluorescent biological samples such as human dental tissues, grape skin, and plum skin due to the suppressed fluorescence interference.

© 2019 The Author(s)

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