Fourier transform infrared spectroscopy (FT-IR) is a well-established and
widely accepted methodology to identify and differentiate diverse microbial species.
In this study, FT-IR was used to differentiate 20 strains of ubiquitous and
agronomically important phytopathogens of <i>Aspergillus flavus</i> and
<i>Aspergillus parasiticus</i>. By analyzing their spectral profiles via
principal component and cluster analysis, differentiation was achieved between the
aflatoxin-producing and nonproducing strains of both fungal species. This study thus
indicates that FT-IR coupled to multivariate statistics can rapidly differentiate
strains of <i>Aspergilli</i> based on their toxigenicity.
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