Abstract

We describe the automatic analysis of fluorescence tracks of phytoplankton recorded with a fluorescence imaging photometer. The optical components and construction of the photometer were described in Part I and Part II of this series in this issue. An algorithm first isolates tracks corresponding to a single phytoplankter transit in the nominal focal plane of a flow cell. Then, the fluorescence streaks in the track that correspond to individual optical elements on the filter wheel are identified. The fluorescence intensity of each streak is integrated and used to calculate ratios. This approach was tested using 853 fluorescence measurements of the coccolithophore <i>Emiliania huxleyi</i> and the diatom <i>Thalassiosira pseudonana</i>. Average intensity ratios for the two classes closely follow those predicted in Part I of this series, with a distribution of ratios in each class that is consistent with the signal-to-noise ratio calculations in Part II for single cells. No overlap of the two class ratios was observed, yielding perfect classification.

Estimation of phytoplankton taxonomic groups in the Arctic Ocean using phytoplankton absorption properties: implication for ocean-color remote sensing

Hailong Zhang, Emmanuel Devred, Amane Fujiwara, Zhongfeng Qiu, and Xiaohan Liu
Opt. Express 26(24) 32280-32301 (2018)

On the discrimination of multiple phytoplankton groups from light absorption spectra of assemblages with mixed taxonomic composition and variable light conditions

Emanuele Organelli, Caterina Nuccio, Luigi Lazzara, Julia Uitz, Annick Bricaud, and Luca Massi
Appl. Opt. 56(14) 3952-3968 (2017)

Diurnal variations of the optical properties of phytoplankton in a laboratory experiment and their implication for using inherent optical properties to measure biomass

Carina Poulin, David Antoine, and Yannick Huot
Opt. Express 26(2) 711-729 (2018)

Inherent optical properties of the coccolithophore: Emiliania huxleyi

Peng-Wang Zhai, Yongxiang Hu, Charles R. Trepte, David M. Winker, Damien B. Josset, Patricia L. Lucker, and George W. Kattawar
Opt. Express 21(15) 17625-17638 (2013)

Precision in imaging multivariate optical computing

Michael N. Simcock and Michael L. Myrick
Appl. Opt. 46(7) 1066-1080 (2007)