Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 66,
  • Issue 1,
  • pp. 60-65
  • (2012)

Linear Discriminant Analysis of Single-Cell Fluorescence Excitation Spectra of Five Phytoplankton Species

Not Accessible

Your library or personal account may give you access

Abstract

<b>Linear discriminant analysis (LDA) of single-cell fluorescence excitation spectra (λ<sub>em</sub> = 680 nm) for five species of marine phytoplankton was used to determine whether intra-species variation among single cells precluded discrimination among species. Single-cell spectra were recorded in an optical trap with a custom-built spectral fluorometer. For nitrogen (N)-replete cells, separation of all five species (<i> Emiliania huxleyi</i>, a coccolithophore,</b> <b><i>Thalassiosira pseudonana</i>, a diatom,</b> <b><i>Dunaliella tertiolecta</i>, a chlorophyte,</b> <b><i>Amphidinium carterae</i>, a dinoflagellate, and</b> <b><i>Rhodomonas salina</i>, a cryptophyte) was possible using only a portion of the excitation spectra (570-610 nm). This wavelength region gave perfect classification of species with a minimum Fisher ratio of 62. For four species (<i> E. huxleyi</i>,</b> <b><i>T. pseudonana</i>,</b> <b><i>D. tertiolecta,</i></b> <b>and</b> <b><i>A. carterae</i>), variations in fluorescence excitation spectra as cells were starved of N did not impact the classification process adversely within the chosen spectral window.</b> <b><i>R. salina</i></b> <b>cells grown with and without N showed significant differences in their fluorescence excitation spectra but could still be classified if a different spectral window (490-570 nm) was used. Overall, we conclude that intra-species variation among single-cell fluorescence excitation spectra does not preclude discrimination among species.</b>

PDF Article
More Like This
On the discrimination of multiple phytoplankton groups from light absorption spectra of assemblages with mixed taxonomic composition and variable light conditions

Emanuele Organelli, Caterina Nuccio, Luigi Lazzara, Julia Uitz, Annick Bricaud, and Luca Massi
Appl. Opt. 56(14) 3952-3968 (2017)

Retrieval of phytoplankton cell size from chlorophyll a specific absorption and scattering spectra of phytoplankton

Wen Zhou, Guifen Wang, Cai Li, Zhantang Xu, Wenxi Cao, and Fang Shen
Appl. Opt. 56(30) 8362-8371 (2017)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved