We report on a model to determine the light transmission of solid particles in immersion liquids. Using the model, optical and physical properties of suspensions such as the refractive index of the particles can be retrieved. The model can also be used in the case of colored suspensions, where the detection of only the maximum transmission would give an inaccurate refractive index for a particle. The validity of the method was tested with CaF<sub>2</sub> particles. Potential applications include scientific studies of the optical properties of micro- and nanoparticles or microorganisms in a liquid matrix. The model is also useful in industry where monitoring of suspensions and colloids is an issue.

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