Abstract
We have constructed a 1064 nm deep near-infrared (NIR) excited multichannel
Raman microspectrometer using an InP/InGaAsP multichannel detector. This
microspectrometer achieves high sensitivity suitable for in vivo measurements of
single living cells with lateral resolution of 0.7 μm and depth resolution of 3.1
μm. It has been applied to the structural analysis of living cyanobacterial cells,
well-known model organisms for photosynthesis research, which are too photolabile to
be measured with visible laser excitation. High signal-to-noise ratio (S/N) Raman
spectra have been obtained from carotenoid, chlorophyll <i>α</i>, and phycocyanin in
a single living cyanobacterial cell with no appreciable interference from
autofluorescence or photodamage. Sub-micrometer mapping of Raman intensities
provides clear distribution images of the three pigments inside the cell.
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