The analysis of body fluid traces during forensic investigations is a critical
step in determining the key details of a crime. Several confirmatory and presumptive
biochemical tests are currently utilized. However, these tests are all destructive,
and no single method can be used to analyze all body fluids. This review outlines
recent progress in the development of a novel universal approach for the
nondestructive, confirmatory identification of body fluid traces using Raman
spectroscopy. The method is based on the use of multidimensional spectroscopic
signatures of body fluids and accounts for the intrinsic heterogeneity of dry traces
and donor variation. The results presented here demonstrate that Raman spectroscopy
has potential for identifying traces of semen, blood, saliva, sweat, and vaginal
fluid with high confidence.
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