Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 62,
  • Issue 6,
  • pp. 649-654
  • (2008)

Evanescent-Wave Cavity Ring-Down Spectroscopy for Enhanced Detection of Surface Binding Under Flow Injection Analysis Conditions

Not Accessible

Your library or personal account may give you access

Abstract

The feasibility of liquid-phase evanescent-wave cavity ring-down spectroscopy (EW-CRDS) for surface-binding studies under flow-injection analysis (FIA) conditions is demonstrated. The EW-CRDS setup consists of an anti-reflection coated Dove prism inside a linear cavity (with standard or super-polishing of the total internal reflective (TIR) surface). A teflon spacer with an elliptical hole clamped on this surface acts as a 20 μL sized flow cell. The baseline noise of this system is of the order of 10<sup>−4</sup> absorbance units; the baseline remains stable over a prolonged time and the prism surface does not become contaminated during repeated injections of the reversibly adsorbing test dyes Crystal Violet (CV) and Direct Red 10 (DR10). At typical FIA or liquid chromatography (LC) flow rates, the system has sufficient specificity to discriminate between species with different surface affinities. For CV a much stronger decrease in ring-down time is observed than calculated based on its bulk concentration and the effective depth probed by the evanescent wave, indicating binding of this positively charged dye to the negatively charged prism surface. The amount of adsorption can be influenced by adjusting the flow rate or the eluent composition. At a flow rate of 0.5 mL/min, an enrichment factor of 60 was calculated for CV; for the poorly adsorbing dye DR10 it is 5. Super-polishing of the already polished TIR surface works counter-productively. The adsorbing dye Crystal Violet has a detection limit of 3 μM for the standard polished surface; less binding occurs on the super-polished surface and the detection limit is 5 μM. Possible applications of EW-CRDS for studying surface binding or the development of bio-assays are discussed.

PDF Article
More Like This
Sensitivity enhancement for evanescent-wave sensing using cavity-ring-down ellipsometry

Dimitris Sofikitis, Katerina Stamataki, Michael A. Everest, Vassilis Papadakis, Jean-Louis Stehle, Benoit Loppinet, and T. Peter Rakitzis
Opt. Lett. 38(8) 1224-1226 (2013)

Monitoring adsorption and sedimentation using evanescent-wave cavity ringdown ellipsometry

Katerina Stamataki, Vassilis Papadakis, Michael A. Everest, Stelios Tzortzakis, Benoit Loppinet, and T. Peter Rakitzis
Appl. Opt. 52(5) 1086-1093 (2013)

Monolithic folded resonator for evanescent wave cavity ringdown spectroscopy

Andrew C. R. Pipino
Appl. Opt. 39(9) 1449-1453 (2000)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved