Abstract
Second-order calibration and multivariate spectroscopic-kinetic measurements in the visible region are proposed to improve the Jaffé method for creatinine assay. Analyses performed on synthetic mixtures containing bilirubin, glucose, and albumin confirm that second-order calibration is useful for creatinine determination in human serum. Quantitative determinations of creatinine with the parallel factor analysis (PARAFAC) and direct trilinear decomposition (TLD) methods were compared. It is shown that both methods can be used for creatinine determination in human serum, with an SEP (squared error of prediction) of 2.22 and coefficient of variability of 6.14% for PARAFAC, and an SEP of 2.38 and coefficient of variability of 6.57% for TLD.
PDF Article
More Like This
Sentinel lymph nodes fluorescence detection and imaging using Patent Blue V bound to human serum albumin
Franklin Tellier, Jérôme Steibel, Renée Chabrier, François Xavier Blé, Hervé Tubaldo, Ravelo Rasata, Jacques Chambron, Guy Duportail, Hervé Simon, Jean-François Rodier, and Patrick Poulet
Biomed. Opt. Express 3(9) 2306-2316 (2012)
Cited By
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Login to access Optica Member Subscription