Abstract

Global metabolic adaptations to physical conditioning were described in 15 subjects by FT-IR spectrometry as the method allowed determination of glucose (Glc), lactate (La), glycerol, triglycerides (TG), fatty acyl moieties (FAM), and total amino acids plasma concentrations. Subtraction of plasma FT-IR spectra obtained at resting state from the exercise spectra also allowed determination of the biomolecular response to exercise. On week 1, exercise induced a transient hypoglycemia, a lactatemia increase of 153%, a FAM depletion of 27%, and a TG concentration decrease of 28%. Protein contents increased by 2%, but these were partly catabolized for amino acid supply (+27%), suggesting an important metabolic stress during exercise. On week 3, exercise hypoglycemia had disappeared, lactate increase was diminished by 91%, TG contents were decreased by 14%, and proteins and amino acids exhibited higher absorption increases. On week 5, TG and FAM concentrations were markedly increased during exercise, protein absorption was still increased (+9%), but amino acid blood release was diminished by 81%. These results described positive adaptations to training. Furthermore, FAM concentration could be determined from plasma FT-IR spectra by using the 2996-2819 cm<sup>-1</sup> spectral area [ν<i><sub>as</sub></i>(CH<sub>3</sub>), ν<i><sub>as</sub></i>(CH<sub>2</sub>), ν<i><sub>s</sub></i>(CH<sub>3</sub>), and ν<i><sub>s</sub></i>(CH<sub>2</sub>) absorbance; 0.82 mMol·L<sup>-1</sup>, a.u. cm<sup>-1</sup>], as well as for amino acid concentration by using the ν(COO<sup>-</sup>) spectral area (1430-1360 cm<sup>-1</sup>; 0.062 g·L<sup>-1</sup>, a.u. × cm<sup>-1</sup>). FT-IR spectrometry was useful to determine simultaneously various plasma concentrations and most of the biomolecular changes through successive samples.

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