The excitation emission matrix spectral subtraction (EEMSS) fluorescence technique was used to estimate adulteration of petrol by kerosene. A calibration graph obtained from the spectral volume with the use of EEMSS fluorescence is found to be efficacious in the whole range 0 to 90% v/v of kerosene. The method is simple, reliable, and superior in terms of both sensitivity (1% v/v) and accuracy compared to any other method. The method gives a good recovery for synthesized adulterated sample.
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