Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 55,
  • Issue 1,
  • pp. 9-22
  • (2001)

Estimation of the BII Conformer Substate Population in Nonoriented Hydrated B-DNA via Curve Resolution of Infrared Spectra

Not Accessible

Your library or personal account may give you access

Abstract

Hydrated films of the self-complementary B-type d(CGCGAATTCGCG)<sub>2</sub> dodecamer, with 13 or 14 water molecules per nucleotide, were studied by Fourier transform infrared (FT-IR) spectroscopy either as equilibrated samples between 290 and 260 K or, after quenching into the glassy state, as nonequilibrated films isothermally at 200 K. IR spectral changes on isothermal relaxation at 200 K revealed that difference spectra are caused by interconversion of B-DNA's conformer substates, B<sub>I</sub> converting into B<sub>II</sub>. Curve resolution of IR spectra in the spectral region containing the symmetric stretching vibration of the ionic phosphate group, with a composite band centered at 1087 cm<sup>-1</sup> for B<sub>I</sub> and at 1109 cm<sup>-1</sup> for B<sub>II</sub>, gives the B<sub>I</sub>/B<sub>II</sub> conformer substate population ratio and its dependence on temperature between 290 and 260 K, and on time for isothermal relaxation at 200 K. Curve resolution of the overlapping composite band profile was optimized by comparison of second-derivative and of difference curves, aiming thereby for optimal correspondence between experimental and curve-fitted bands as a criterion for goodness-of-fit. The spectroscopic aspects relevant for reliable curve resolution of the overlapping composite band profile are reported and discussed in detail.

PDF Article
More Like This
Excited-state conformational dynamics of nickel (II) porphyrins

S. H. Courtney, T. M. Jedju, J. M. Friedman, L. Rothberg, R. G. Alden, M. S. Park, and M. R. Ondrias
J. Opt. Soc. Am. B 7(8) 1610-1614 (1990)

Recent applications of FT-IR spectroscopy to polymer systems

J. L. Koenig and M. K. Antoon
Appl. Opt. 17(9) 1374-1385 (1978)

Estimation of component spectral curves from unknown mixture spectra

Keiji Sasaki, Satoshi Kawata, and Shigeo Minami
Appl. Opt. 23(12) 1955-1959 (1984)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved