Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 54,
  • Issue 7,
  • pp. 963-967
  • (2000)

Comparative Two-Dimensional Fourier Transform Infrared Correlation Spectroscopic Study on the Spontaneous, Pressure-, and Temperature-Enhanced H/D Exchange in α-Lactalbumin

Not Accessible

Your library or personal account may give you access

Abstract

Two-dimensional Fourier transform infrared FT-IR was applied for the examination of the H/D exchange process in D<sub>2</sub>O solutions of bovine α-lactalbumin. The slow spontaneous H/D exchange at 25 °C was compared with pressure-enhanced and temperature-enhanced H/D exchange. The H/D-exchange process, which takes 48 h at 25 °C and under atmospheric pressure, is completed within 3 h when pressure is gradually elevated up to 180 MPa, or when temperature is increased up to 42.5 °C. Although the slow room-temperature H/D exchange and the fast pressure or temperature-enhanced H/D exchange feature a high degree of similarity between the corresponding second-derivative spectra, the two-dimensional (2D) FT-IR correlation maps revealed that upon the heat treatment the order in which the H/D exchange takes place in the structural domains of α-lactalbumin is altered. It has been observed that these differences can be explained in light of the known properties of α-lactalbumin and the crystallographic data.

PDF Article
More Like This
Fourier transform infrared spectroscopic study of molecular interactions in hemoglobin

James O. Alben and George H. Bare
Appl. Opt. 17(18) 2985-2990 (1978)

Ultrafast nonequilibrium Fourier-transform two-dimensional infrared spectroscopy

Carlos R. Baiz, Matthew J. Nee, Robert McCanne, and Kevin J. Kubarych
Opt. Lett. 33(21) 2533-2535 (2008)

Broadband in-line terahertz 2D imaging: comparative study with time-of-flight, cross-correlation, and Fourier transform data processing

Aleksandr Ushakov, Pavel Chizhov, Vladimir Bukin, Andrei Savel’ev, and Sergei Garnov
J. Opt. Soc. Am. B 35(5) 1159-1164 (2018)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved