Abstract

A nondestructive analytical method based on the use of Fourier transform Raman spectroscopy (FT-RS) for identification and quantitative analysis of hydroxyapatite (HAP) and collagen (COL) in bones was developed. A calibration curve based on the Raman spectra of known powder mixtures of HAP-COL was constructed with the use of the Raman bands at 960 cm^-1 for HAP and 2937 cm^-1 for COL. It was found that <i>I</i>⁹⁶⁰/<i>I</i>²⁹³⁷ = 0.46/W_c - 0.36, where I⁹⁶⁰ and I²⁹³⁷ are the relative intensities for the 960 and 2937 cm^-1 Raman bands, respectively, and W_c represents the weight fraction of collagen. The detection limit for HAP and COL was found to be 2.8 and 0.6 wt %, respectively. The potential of using RS for an <i>in vitro</i> point-by-point analysis ("mapping") of bones was demonstrated successfully on an artificially demineralized ox femur sample.

Biochemical characterization of human gingival crevicular fluid during orthodontic tooth movement using Raman spectroscopy

Gyeong Bok Jung, Kyung-A Kim, Ihn Han, Young-Guk Park, and Hun-Kuk Park
Biomed. Opt. Express 5(10) 3508-3520 (2014)

Spatially-offset Raman spectroscopy for monitoring mineralization of bone tissue engineering scaffolds: feasibility study based on phantom samples

Max Dooley, Aruna Prasopthum, Zhiyu Liao, Faris Sinjab, Jane McLaren, Felicity R. A. J. Rose, Jing Yang, and Ioan Notingher
Biomed. Opt. Express 10(4) 1678-1690 (2019)

Cortical bone quality affectations and their strength impact analysis using holographic interferometry

Cesar G. Tavera Ruiz, Manuel H. De La Torre-Ibarra, J. M. Flores-Moreno, Claudio Frausto-Reyes, and Fernando Mendoza Santoyo
Biomed. Opt. Express 9(10) 4818-4833 (2018)

Spatially offset Raman spectroscopy for in vivo bone strength prediction

Chi Shu, Keren Chen, Maria Lynch, Jason R. Maher, Hani A. Awad, and Andrew J. Berger
Biomed. Opt. Express 9(10) 4781-4791 (2018)

Photothermal tomography for the functional and structural evaluation, and early mineral loss monitoring in bones

Sreekumar Kaiplavil, Andreas Mandelis, Xueding Wang, and Ting Feng
Biomed. Opt. Express 5(8) 2488-2502 (2014)