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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 53,
  • Issue 9,
  • pp. 1118-1122
  • (1999)

Optical Absorption and Fluorescence Spectral Imaging Using Fiber Bundle Image Compression

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Abstract

Optical absorption and fluorescence spectral imaging is performed by using the recently developed fiber-bundle image compression (FIC) technique in which an entire spectral image is collected in a single scan of a charged-coupled device (CCD) detector. Absorption imaging is demonstrated by mapping the optical absorbance of a stained microscopic lily plant stem section. Fluorescence imaging is demonstrated by mapping shifts in the ruby R<sub>1</sub> fluorescence line to determine the pressure distribution in a microcrystalline ruby powder squeezed between two diamond surfaces. The advantages and limitations of the FIC method, relative to tunable filter and line imaging techniques, are discussed.

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