A method to correct for spectral variations in multiwavelength responses using a photon time-of-flight measurement was investigated. A new approach is described where near-infrared spectra of various absorbing and scattering solutions are corrected for nonlinear variations in the measured attenuation. The spectral attenuation measured in a sample is related to the concentration of the analyte as well as the scattering from the medium. The dependence of the detected attenuation on the absorption and scattering at a single wavelength can be described by the steady-state diffusion approximation. With the use of the steady-state diffusion approximation, single-wavelength correction methodologies for multiwavelength responses were developed to refine constituent estimates in a scattering medium. The single-wavelength corrections examined the use of multiwavelength responses ratioed to the absorption and scattering determined at a single wavelength to reduce the scattering influence in the detected attenuations. The single-wavelength correction method improved constituent estimates in a variable medium. The correction utilized the absorption and scattering determined from processed time-resolved photon distributions at a single wavelength. Partial least-squares (PLS) regression was used to obtain constituent absorption estimates from diffuse transmittance spectra of turbid samples. A 70% improvement over PLS-processed spectra was obtained with a combined single-wavelength absorption and scattering correction. In comparison to steady-state multiwavelength correction methods such as wavelength normalization, derivative spectroscopy, and multiplicative signal correction, the results demonstrate a significant improvement in estimates of the dye concentration. Results suggest a practical method to correct for scatter variations in multiwavelength responses for routine spectroscopic chemical analysis of turbid samples.

PDF Article

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
Login to access OSA Member Subscription