Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 51,
  • Issue 6,
  • pp. 836-843
  • (1997)

Near-Infrared Heptamethine Cyanine Dyes: A New Tracer for Solid-Phase Immunoassays

Not Accessible

Your library or personal account may give you access

Abstract

Near-infrared (near-IR) fluorescence has been used to develop a solid-phase immunoassay that detects trace amounts of human immunoglobulin (HuIgG). Various concentrations of HuIgG bound to a nitrocellulose surface were determined from the fluorescence generated by near-IR labeled goat anti-human antibody (GAHG) bound to the HuIgG. The GAHG was labeled with a heptamethine cyanine fluorophore that has spectral properties in the near-IR region (above 780 nm). These fluorophores are versatile because they can be modified for several bioanalytical applications. Fluorescence was detected with a near-IR fluorescence instrument previously developed in the laboratory. Two cyanine fluorophore labels were evaluated for the ability to selectively bind to GAHG on a nitrocellulose matrix with a minimal amount of background interference. After the most appropriate near-IR fluorophore was selected, the labeling of GAHG was optimized under aqueous conditions. The most effective GAHG-dye conjugates were used to develop an immunoassay to detect various concentrations of HuIgG. The results are presented, here. Solutions of HuIgG with concentrations as low as 10-10 molar have been detected with a minimum of interference.

PDF Article
More Like This
Photoconductivity of Some Cyanine Dyes*

R. C. Nelson
J. Opt. Soc. Am. 46(1) 10-13 (1956)

Morphological resonances for multicomponent immunoassays

W. B. Whitten, M. J. Shapiro, J. M. Ramsey, and B. V. Bronk
Appl. Opt. 34(18) 3203-3207 (1995)

Quantitative analysis of liquid crystal-based immunoassay using rectangular capillaries as sensing platform

Jhih-Wei Huang, Hideaki Hisamoto, and Chih-Hsin Chen
Opt. Express 27(12) 17080-17090 (2019)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved