A fluorimetric study on the spectral characteristics and acid-base behavior of 7-hydroxymethylnalidixic acid has been performed. The host-guest complexation process between gamma -cyclodextrin and 7-hydroxymethylnalidixic acid has also been investigated by fluorescence spectroscopy. Scatchard and Benesi-Hildebrand methods have been applied to determine the stoichiometry of the complex, and a 1:1 stoichiometric ratio has been established. A K1 = 118 +/- 25 M -1 formation constant has been calculated for the inclusion complex, by using the changes produced on the fluorescence of 7-hydroxymethylnalidixic acid, when it is included on the hydrophobic cyclodextrin cavity. A fluorimetric method of determination of 7-hydroxymethylnalidixic acid, in gamma -cyclodextrin aqueous solution, has been proposed. The analytical parameters and detection and quantification limits of the method have been determined.
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
Login to access OSA Member Subscription