Abstract

A novel fluorimeter has been developed that combines the selectivity of time-resolved detection and the sample throughput of a 96-well plate reader. With improved background rejection via temporal resolution, a reduction in the limit of detection by a factor of 600 was attained over that of a commercially available 96-well plate fluorimeter. The excellent limit of detection was utilized to differentiate two closely related species of Pseudomonas , P. fluorescens and P. aureofaciens , at a bacterial concentration of 500,000 cells per well with only 15 min of incubation. High sample throughput was demonstrated by incubation of six bacteria samples and data collection in an amino acid 12-nutrient profile within 30 min.

Integrated multi-channel optical system for bacteria characterization and its potential use for monitoring of environmental bacteria

Igor Buzalewicz, Agnieszka Suchwałko, Paweł Trzciński, Lidia Sas-Paszt, Beata Sumorok, Katarzyna Kowal, Ryszard Kozera, Alina Wieliczko, and Halina Podbielska
Biomed. Opt. Express 10(3) 1165-1183 (2019)

Excitation-emission fluorimeter based on linear interference filters

Michael Gouzman, Nadia Lifshitz, Serge Luryi, Oleg Semyonov, Dmitry Gavrilov, and Vyacheslav Kuzminskiy
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Principal component analysis of fluorescence changes upon growth conditions and washing of Pseudomonas aeruginosa

Ekta Jhala, Craig Galilee, and Lou Reinisch
Appl. Opt. 46(22) 5522-5528 (2007)

Towards the clinical application of laser-induced breakdown spectroscopy for rapid pathogen diagnosis: the effect of mixed cultures and sample dilution on bacterial identification

Steven J. Rehse, Qassem I. Mohaidat, and Sunil Palchaudhuri
Appl. Opt. 49(13) C27-C35 (2010)

Detection of bacteria by time-resolved laser-induced breakdown spectroscopy

Stéphane Morel, Nicolas Leone, Philippe Adam, and Jacques Amouroux
Appl. Opt. 42(30) 6184-6191 (2003)