Abstract

An ultrafast laser apparatus has been developed that allows time-resolved UV-visible and infrared spectroscopy to be performed with a time resolution of 200 fs. The instrument design allows UV-vis and IR data to be collected either as spectra or as kinetic profiles. Solution flow and cell translation sampling systems have been developed that allow the handling of chemical and biochemical systems, including highly air-sensitive materials and volumes as small as 50 μL. The apparatus performance has been evaluated with the use of <i>trans</i>-stilbene in hexane (UV-vis) and silicon (IR) test samples, and the techniques have been applied in combination to study both native carbonmonoxymyoglobin and the novel H64L mutant.

An Apparatus for Time-Resolved Spectroscopy

G. Gordon and W. M. Cady
J. Opt. Soc. Am. 40(12) 852-853 (1950)

Ultrafast continuum mid-infrared spectroscopy: probing the entire vibrational spectrum in a single laser shot with femtosecond time resolution

Carmella Calabrese, Ashley M. Stingel, Lei Shen, and Poul B. Petersen
Opt. Lett. 37(12) 2265-2267 (2012)

Picosecond time-resolved vibrational spectroscopy using a regenerative amplifier

T. M. Jedju, M. W. Roberson, and L. Rothberg
Appl. Opt. 31(15) 2684-2691 (1992)

Subpicosecond time-resolved vibrational spectroscopy by transient infrared absorption

T. M. Jedju, L. Rothberg, and A Labrie
Opt. Lett. 13(11) 961-963 (1988)

Tunable ultrafast infrared/visible laser to probe vibrational dynamics

Tissa C. Gunaratne, Matthew Milliken, J. Reddy Challa, and M. Cather Simpson
Appl. Opt. 45(3) 558-564 (2006)