Abstract

The rates of interactions of polycyclic aromatic hydrocarbons (PAHs) with biological cells studied with fluorescence appear to be different for cells from different origins. The monomer emission of benzo[<i>a</i>]pyrene (BaP) is enhanced as in liposomes, but more significantly in normal liver cells than in liver cancer cells or kidney cells, and that enhancement is proportional to the amount of cells added. When PAHs are allowed to interact with cells for a certain period of time, metabolism appears to occur. The excimer emission is seen to dissipate continuously as reactions proceed, whereas the monomer emission increases, passes through a maximum, then starts to decrease when excimer emission becomes exhausted. The time plot of the BaP excimer emission in semilogarithmic coordinates indicates that the decrease or the mass transfer of microcrystalline BaP to the cell membrane is a first-order process. Metabolism has been investigated by monitoring the monomer emission. Liver cells have higher monomer emission than the kidney cells in the early stages of interaction, indicating that these cell membranes are more amenable to PAH absorption. Effects of vitamin K₃ and radiation have also been investigated.

Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells

Zoran Arsov, Iztok Urbančič, Maja Garvas, Daniele Biglino, Ajasja Ljubetič, Tilen Koklič, and Janez Štrancar
Biomed. Opt. Express 2(8) 2083-2095 (2011)

Interference filter tilting to detect a polycyclic aromatic hydrocarbon at the second harmonic of wavelength modulation frequency

Dmitri Lanevski, Koit Mauring, and Eric Tkaczyk
Appl. Opt. 56(11) 3155-3161 (2017)

Simultaneous one-dimensional visualization of OH, polycyclic aromatic hydrocarbons, and soot in a laminar diffusion flame

Francesco Cignoli, Sergio Benecchi, and Giorgio Zizak
Opt. Lett. 17(4) 229-231 (1992)

Unsupervised clustering of multiparametric fluorescent images extends the spectrum of detectable cell membrane phases with sub-micrometric resolution

Giada Bianchetti, Marco De Spirito, and Giuseppe Maulucci
Biomed. Opt. Express 11(10) 5728-5744 (2020)

Surface plasmon-enhanced two-photon fluorescence microscopy for live cell membrane imaging

Ruei-Yu He, Yuan-Deng Su, Keng-Chi Cho, Chun-Yun Lin, Nan-Shan Chang, Chih-Han Chang, and Shean-Jen Chen
Opt. Express 17(8) 5987-5997 (2009)