This study demonstrates the potential of anti-Stokes Raman spectroscopy in investigating biological samples in a nondestructive manner; quantitative analysis of glucose in plasma and serum has been studied as an example. The efficient collection of anti-Stokes Raman scattering by use of chromatic aberration of a lens has allowed us to obtain high-quality anti-Stokes Raman spectra from glucose in plasma and serum, which is a strongly fluorescent biological sample. The concentration of glucose in these materials can be estimated by the anti-Stokes Raman intensity of the band at 1130 cm<sup>-1</sup> due to the C-O stretching mode. The correlation coefficient between the concentration and the intensity has been calculated to be 0.993 and 0.991 for glucose in plasma and serum, respectively. The detection limits for these materials have been found to be 45 mg/dL and 50 mg/dL, respectively.
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