Abstract

The adsorption behavior of high-density lipoprotein (HDL) and low-density lipoprotein (LDL) onto a zinc selenide crystal surface and cholesterol-modified ZnSe crystal surface has been studied in terms of adsorption kinetics and isotherms with the use of the FT-IR/ATR technique. Adsorption kinetic plots indicated that the adsorption equilibrium of HDL and LDL is reached at 30-40 min, which is similar to the case for other proteins. From the adsorption isotherms of HDL and LDL, transitions from monolayer adsorption to multilayer adsorption were observed at around 10^-5 M for both lipoproteins. We also observed that the binding of HDL and LDL to a cholesterol-modified ZnSe surface is less than that to an unmodified ZnSe surface. For example, monolayer adsorption density of HDL on the ZnSe surface was 0.23 pmoles/cm², whereas it was 0.13 pmoles/cm² on the cholesterol-modified ZnSe surface.

Novel fluorescent probe for low density lipoprotein, based on the enhancement of Europium emission band

L. C. Courrol, A.M. Monteiro, F.R.O. Silva, L. Gomes, N.D. Vieira, M.A. Gidlund, and A.M. Figueiredo Neto
Opt. Express 15(11) 7066-7074 (2007)

Recent applications of FT-IR spectroscopy to polymer systems

J. L. Koenig and M. K. Antoon
Appl. Opt. 17(9) 1374-1385 (1978)

Theoretical determination of parameters for optimum surface specificity in overlayer attenuated-total-reflection infrared spectroscopy

John S. Loring and Donald P. Land
Appl. Opt. 37(16) 3515-3526 (1998)

Use of attenuated total reflectance Fourier transform infrared spectroscopy to monitor the development of lipid aggregate structures

Mateo R. Hernandez, Elyse N. Towns, Terry C. Ng, Brian C. Walsh, Richard Osibanjo, Atul N. Parikh, and Donald P. Land
Appl. Opt. 51(15) 2842-2846 (2012)

Infrared characterization of surfaces and coatings by internal-reflection spectroscopy

E. D. Palik, J. W. Gibson, R. T. Holm, M. Hass, M. Braunstein, and B. Garcia
Appl. Opt. 17(11) 1776-1785 (1978)