Abstract

In this paper, the sensitivity of three-dimensional fluorescence spectrometry (TDFS) is studied by using the total fluorescence intensity, calculated by Monte Carlo integration, instead of conventional single-point intensity. It has been proven that the sensitivity of the total fluorescence intensity method is nearly one hundred times better than that of the single-point intensity method for the determinations of fluorescein and tryptophan. The mechanism of the method has also been studied.

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