Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 47,
  • Issue 1,
  • pp. 103-110
  • (1993)

Maximum-Likelihood Restoration Data Processing Techniques Applied to Matrix-Assisted Laser Desorption Mass Spectra

Not Accessible

Your library or personal account may give you access

Abstract

Maximum-likelihood restoration (MLR) data processing is applied to matrix-assisted laser desorption time-of-flight mass spectra of several peptide/protein samples. It is shown that this technique can enhance mass resolution for peptides (porcine insulin; MW = 5777.58 Da) sufficiently to assist in the identification of various matrix adduction species formed in the laser desorption process. For higher-molecular-weight proteins, mass resolution enhancement is such that peak overlap contributions from partially resolved matrix adduct ion species can be minimized. Mass accuracies are improved by approximately a factor of six for a sample of a bacterial protease (Subtilisin Carlsberg; Subtilopeptidase <i>A</i>) from <i>Bacillus licheniformis</i> (MW = 27,288.4 Da). The MLR technique also supports the contention that the mass resolution degradation observed with increasing analyte mass is at least partially due to ion signal tailing, presumably caused by secondary ion species being produced in the detection process. This factor is also postulated as being at least partially responsible for the lack of success at resolution enhancement for higher mass analytes (bovine serum albumin; MW = 66,430.3 Da) where the multiple matrix adduction species produced can no longer be resolved from the protonated molecular ion species, thus limiting experimental mass measurement accuracies.

PDF Article
More Like This
Molecular analysis by ionization of laser-desorbed neutral species

Keith R. Lykke, Peter Wurz, Deborah H. Parker, and Michael J. Pellin
Appl. Opt. 32(6) 857-866 (1993)

Surface profiling of lithium with a laser-desorption mass-spectrometer microprobe

Grant A. Bickel and Harry M. Adams
Appl. Opt. 38(9) 1488-1493 (1999)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved