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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 46,
  • Issue 6,
  • pp. 945-947
  • (1992)

On-Line Detection in Slab Gel Electrophoresis Using Thermal Lens Spectrophotometry

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Abstract

Thermal lens spectrophotometry was used to detect hemoglobin and cytochrome <i>c</i> within an acrylamide gel matrix both during electrophoresis and in static experiments in which each protein was incorporated in the matrix during polymerization. For cytochrome <i>c,</i> the detection limit was 0.02 mg/mL for static experiments (approximately 0.5 ng in the laser beam volume), while a detection limit of 180 mg (5 ng in the beam volume) was found for electrophoresis runs.

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