The relationship between the relative fluorescence signal excited and collected with a double-fiber optic sensor and the sample depth has been investigated. The complexity of the analytical expressions for the relative fluorescence signal and the effective depth was reduced by deriving a set of semi-empirical equations which can be evaluated in a simple fashion. These expressions take into account the configuration of the sensor, i.e., fiber diameter, acceptance angle, and separation between fibers. The expressions were tested with the use of double-fiber sensors with different diameters and separations between fibers. The reduction of the effective depth in solutions with significant absorbance was evaluated.

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