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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 46,
  • Issue 4,
  • pp. 704-706
  • (1992)

Multiple Interacting Factors Determine Precision in Derivative Ultraviolet Spectrometry

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Abstract

Theory and experiment show that the precision of quantitative derivative ultraviolet spectrometry depends on the choice of derivative order, degree of smoothing, convolution method of smoothing or derivatization, and power of the reference beam. Except for work describing the simultaneous effect of wavelength range and derivative order, investigations have been made by varying the level of one factor at a time. Adjustment of a single factor at a time is not an efficient way of establishing optimum conditions for analysis, particularly in derivative ultraviolet spectrometry.

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