Abstract

A practical system for UV resonance Raman (RR) measurements based on the combined use of the first-/second-order dispersions of two gratings in an ordinary double monochromator is proposed. This system rejects visible stray light completely and gives four times larger throughput than the combination of the normal second-order dispersion of two gratings and a visible-cut filter. Newly designed <i>f</i>/1.1 Cassegrainian mirror optics and a sensitive spinning cell using an ESR sample tube are combined with this spectrometer and applied to measurements of UV RR spectra of hen egg white lysozyme. The continuous-wave 244-nm excited spectra of lysozyme yielded Raman bands of tryptophan (Trp) and deprotonated tyrosine (Tyr^-) residues in 10 min with high signal-to-noise ratios, and there was no sample degradation. It is demonstrated that the intensity of the Tyr^- <i>v</i>_8<i>a</i> band in the 244-nm excited spectrum can be used to quantify the number of deprotonated Tyr residues in the stepwise ionization of a protein.

Deep UV resonant Raman spectroscopy for photodamage characterization in cells

Yasuaki Kumamoto, Atsushi Taguchi, Nicholas Isaac Smith, and Satoshi Kawata
Biomed. Opt. Express 2(4) 927-936 (2011)

Raman scattering and red fluorescence in the photochemical transformation of dry tryptophan particles

Chih Wei Lai, Mark Schwab, Steven C. Hill, Joshua Santarpia, and Yong-Le Pan
Opt. Express 24(11) 11654-11667 (2016)

Spectral features of Trp-Trp dipeptides using PSSS-templated silver nanoparticles

Yufeng Yuan, Junle Qu, Jun Song, Maozhen Xiong, and Junxian Liu
Opt. Mater. Express 6(1) 146-154 (2016)

Multiple Monochromators. III. A Quadruple Monochromator and Its Application to Infrared Spectroscopy

N. S. Ham, A. Walsh, and J. B. Willis
J. Opt. Soc. Am. 42(7) 496-500 (1952)

Fiber-optic probes with improved excitation and collection efficiency for deep-UV Raman and resonance Raman spectroscopy

L. Shane Greek, H. Georg Schulze, Michael W. Blades, Charles A. Haynes, Karl-Friedrich Klein, and Robin F. B. Turner
Appl. Opt. 37(1) 170-180 (1998)