Abstract

Troponin C (TnC) is covalently labeled with a fluorescent probe molecule dansylaziridine and immobilized on polyethyleneimine-impregnated cellulose. An optrode is constructed by attaching the treated cellulose membrane to the distal end of a bifurcated fiber-optic cable. Because fluorescence is enhanced as Ca⁺² binds with the immobilized dansylated troponin (TnC_DANZ), this system can be used as a calcium sensor for monitoring plasma Ca⁺² levels.

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