Abstract

There is much interest in the biological and pharmacological sciences in knowing the specific location, concentration, and chemical structure of biologically active molecules in tissue. Infrared spectroscopy can be a suitable method for addressing these considerations; it can give quantitative and qualitative information without destroying the sample. IR spectra of approximately 20-μm-thick slices of animal tissue, mounted on barium or calcium fluoride crystals, can be obtained in the transmittance mode. Also, 3–5-μm-thick tissue slices can be mounted on gold-coated glass slides, and IR spectra can be obtained in the reflection-absorption mode.

Comparison of fluorescence lifetime and multispectral imaging for quantitative multiplexing in biological tissue

Rahul Pal and Anand T. N. Kumar
Biomed. Opt. Express 13(7) 3854-3868 (2022)

Platform for quantitative multiscale imaging of tissue composition

Michael A. Pinkert, Zachary J. Simmons, Ryan C. Niemeier, Bing Dai, Lauren B. Woods, Timothy J. Hall, Paul J. Campagnola, Jeremy D. Rogers, and Kevin W. Eliceiri
Biomed. Opt. Express 11(4) 1927-1946 (2020)

Recent applications of FT-IR spectroscopy to polymer systems

J. L. Koenig and M. K. Antoon
Appl. Opt. 17(9) 1374-1385 (1978)

Modeling phase functions in biological tissue

Wei Gong, Ke Si, and Colin J. R. Sheppard
Opt. Lett. 33(14) 1599-1601 (2008)

Modulation transfer function cascade model for a sampled IR imaging system

Luigi de Luca and Gennaro Cardone
Appl. Opt. 30(13) 1659-1664 (1991)