Abstract

The advantage of low-temperature Raman spectroscopy is well known, and this technique is now widely used for many different types of compounds. The recent interest in resonance Raman spectroscopy applied to the biological compounds posed new demands for the experimental techniques. Several valuable improvements have been made in this area, especially for samples which require handling under anaerobic conditions. In 1986 Czernuszewicz presented an anaerobic sample cell use in conjunction with a closed-cycle helium refrigerator. This type of cell has been successfully applied to the resonance Raman spectra collected under back-scattering geometry with incident radiation at 135° to the surface of the sample. However, this arrangement is not suitable for collection of scattering at 90° to the incident beam, the conventional arrangement of the majority of commercial Raman spectrometers. Thus a simple low-temperature anaerobic cell suitable for 90° scattering geometry has been designed, and it is presented here.

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