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Optica Publishing Group
  • Applied Spectroscopy
  • Vol. 42,
  • Issue 8,
  • pp. 1365-1368
  • (1988)

MAGIC-LC/FT-IR Spectrometry: Preliminary Studies

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Abstract

A new solvent elimination interface based on the Monodisperse Aersol Generation Interface for Combining Liquid Chromatography with Fourier transform infrared (MAGIC-LC/FT-IR) spectrometry is described. The solvent elimination efficiency of MAGIC-LC/FT-IR was studied by varying the mobile-phase composition from 100% methanol to 100% water. As the mobile-phase composition was varied, erythrosin B was injected into the interface and deposited on a KBr window after the solvent removal. Spectra were obtained which compared favorably with reference spectra, even as the mobile-phase water content was increased. A reverse-phase separation was completed to demonstrate that readily identifiable spectra can be obtained from mobile phases containing high percentages of water, without heating of the effluent stream.

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