Abstract

Heat denaturation of albumin aqueous solution enables us to control the α-helix content to a reasonable level, and denatured albumin, whose conformation is different, can be obtained. Fourier transform-infrared absorption spectra in the amide I, II, and III band regions of these albumins have been measured and compared with each other. As a result, the characteristic frequencies associated with each conformation of α-helix, β-sheet, and random structure have been observed; moreover it has been newly proved that the intensity ratio of the amide II band to the amide I band depends on the α-helix content in albumin. In this paper, we demonstrate that this intensity ratio is very useful for quantitative estimation of α-helix content in albumin and apply this method to analysis of ATR spectra of albumin adsorbed on polyethylene surface.

Quantitative characterization of bovine serum albumin thin-films using terahertz spectroscopy and machine learning methods

Yiwen Sun, Pengju Du, Xingxing Lu, Pengfei Xie, Zhengfang Qian, Shuting Fan, and Zexuan Zhu
Biomed. Opt. Express 9(7) 2917-2929 (2018)

Changes in porphyrin nonlinear absorption owing to interaction with bovine serum albumin

Iouri E. Borissevitch, Nikifor Rakov, Glauco S. Maciel, and Cid B. de Araújo
Appl. Opt. 39(24) 4431-4435 (2000)

Biochemical characterization of human gingival crevicular fluid during orthodontic tooth movement using Raman spectroscopy

Gyeong Bok Jung, Kyung-A Kim, Ihn Han, Young-Guk Park, and Hun-Kuk Park
Biomed. Opt. Express 5(10) 3508-3520 (2014)

Fabrication of gold nanorods-doped, bovine serum albumin microstructures via multiphoton excited photochemistry

Chi-Hsiang Lien, Wen-Shuo Kuo, Keng-Chi Cho, Chun-Yu Lin, Yuan-Deng Su, Lynn L. H. Huang, Paul J. Campagnola, Chen Yuan Dong, and Shean-Jen Chen
Opt. Express 19(7) 6260-6268 (2011)

Bovine serum albumin label-free concentration sensor based on silica corrosion quantitative monitoring system

Jinjian Li, Yi Liu, Yan Li, Zhenrong Zhang, and Shiliang Qu
Opt. Express 30(12) 21725-21735 (2022)