A novel, compact He-Ne laser intracavity photothermal beam deflection densitometer has been developed. In this instrument, a TLC plate is placed inside the cavity of the He-Ne laser, which serves as a probe. An argon-ion pump-laser overlaps with the probe beam at the chromatogram spot. The apparatus is 34 times more sensitive than the corresponding extracavity instrument. The technique not only was able to detect bilirubin, bilirubin ditauride, and half-bilirubin on a developed HPTLC but also provided insight into the photophysical processes of those pigments. The detection limit for bilirubin, excited by the 476-nm laser line, at 20 mW intensity, modulated at 7 Hz, is 2.5 ng.
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
Login to access OSA Member Subscription